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Ntrk2 (NM_008745) Mouse Untagged Clone
CAT＃: MC216473
Ntrk2 (untagged) - Mouse neurotrophic tyrosine kinase, receptor, type 2 (Ntrk2), transcript variant 2, (10ug)
CNY 3,656.00
CNY 6,180.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | GP145-TrkB/GP95-TrkB; Tkrb; trk-B; trkB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC216473 representing NM_008745
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCCCTGGCTGAAGTGGCATGGACCCGCCATGGCGCGGCTCTGGGGCTTATGCCTGCTGGTCTTGG GCTTCTGGAGGGCCTCTCTCGCCTGCCCGACGTCCTGCAAATGCAGTTCCGCTAGGATTTGGTGTACTGA GCCTTCTCCAGGCATCGTGGCATTCCCGAGGTTGGAACCTAACAGCGTTGACCCGGAGAACATCACGGAA ATTCTCATTGCAAACCAGAAAAGGCTAGAAATCATCAATGAAGATGACGTTGAAGCTTACGTGGGGCTGA GAAACCTTACAATTGTGGATTCCGGCTTAAAGTTTGTGGCTTACAAAGCGTTTCTGAAAAACAGCAACCT GCGGCACATAAATTTCACACGAAACAAGCTGACGAGTTTGTCCAGGAGACATTTCCGCCACCTTGACTTG TCTGACCTGATCCTGACGGGTAATCCGTTCACGTGCTCCTGCGACATCATGTGGCTCAAGACTCTCCAGG AGACTAAATCCAGCCCCGACACTCAGGATTTGTACTGCCTCAATGAGAGCAGCAAGAACATGCCCCTGGC GAACCTGCAGATACCCAATTGTGGTCTGCCATCTGCACGTCTGGCTGCTCCTAACCTCACCGTGGAGGAA GGAAAGTCTGTGACCCTTTCCTGCAGTGTGGGGGGTGACCCACTCCCCACCTTGTACTGGGACGTTGGGA ATTTGGTTTCCAAGCACATGAATGAAACAAGCCACACACAGGGCTCCTTAAGGATAACGAACATTTCATC TGATGACAGTGGAAAGCAAATCTCTTGTGTGGCAGAAAACCTTGTAGGAGAAGATCAAGATTCTGTGAAC CTCACTGTGCATTTTGCGCCAACTATCACGTTTCTCGAGTCTCCAACCTCAGATCACCACTGGTGCATTC CATTCACTGTGAGAGGCAACCCCAAGCCTGCGCTTCAGTGGTTCTACAATGGGGCCATACTGAATGAGTC CAAGTACATCTGTACTAAGATCCACGTCACCAATCACACGGAGTACCATGGCTGCCTCCAGCTGGATAAC CCCACTCATATGAATAACGGAGACTACACCCTGATGGCCAAGAACGAGTATGGGAAGGATGAGAGACAGA TCTCCGCTCACTTCATGGGCCGGCCTGGAGTCGACTACGAGACAAACCCAAATTACCCTGAAGTCCTCTA TGAAGACTGGACCACGCCAACTGACATTGGGGATACTACGAACAAAAGTAATGAAATCCCCTCCACGGAT GTTGCTGACCAAAGCAATCGGGAGCATCTCTCGGTCTATGCCGTGGTGGTGATTGCATCTGTGGTGGGAT TCTGCCTGCTGGTGATGTTGCTCCTGCTCAAGTTGGCGAGACATTCCAAGTTTGGCATGAAAGGTTTTGT TTTGTTTCATAAGATCCCACTGGATGGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_008745 |
| Insert Size | 1431 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC052014, AAH52014 |
| RefSeq Size | 2279 bp |
| RefSeq ORF | 1431 bp |
| Locus ID | 18212 |
| UniProt ID | P15209 |
| Gene Summary | Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. Isoform GP95-TRKB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks several exons and uses an alternate 3'-terminal exon, compared to variant 1. The encoded isoform (b) has a shorter and distinct C-terminus, compared to isoform a. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226132 | Ntrk2 (tGFP-tagged) - Mouse neurotrophic tyrosine kinase receptor type 2 (Ntrk2) transcript variant 2, (10ug) |
CNY 7,456.00 |
|
| MR226132 | Ntrk2 (Myc-DDK-tagged) - Mouse neurotrophic tyrosine kinase, receptor, type 2 (Ntrk2), transcript variant 2 |
CNY 4,750.00 |
|
| MR226132L3 | Lenti ORF clone of Ntrk2 (Myc-DDK-tagged) - Mouse neurotrophic tyrosine kinase, receptor, type 2 (Ntrk2), transcript variant 2 |
CNY 6,650.00 |
|
| MR226132L4 | Lenti ORF clone of Ntrk2 (mGFP-tagged) - Mouse neurotrophic tyrosine kinase, receptor, type 2 (Ntrk2), transcript variant 2 |
CNY 6,650.00 |
