Gabrb3 (NM_001038701) Mouse Untagged Clone
CAT#: MC216454
Gabrb3 (untagged) - Mouse gamma-aminobutyric acid (GABA) A receptor, subunit beta 3 (Gabrb3), transcript variant 2, (10ug)
CNY 6,180.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | A230092K12Rik; AW049585; beta3; Cp1; Gabrb-3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC216454 representing NM_001038701
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCTCCGGGCTCCAGGCCCTGCTGCTGCCAATCTGGCTTTCCTGGACCCTGGGGACGCGAGGATCTG AGCCCAGCAGCGTAAACGACCCCGGGAACATGTCCTTTGTGAAGGAGACGGTCGACAAGCTGTTGAAAGG CTACGACATTCGCCTGAGACCCGACTTCGGGGGTCCCCCAGTCTGCGTGGGGATGAACATCGACATCGCC AGCATCGACATGGTTTCTGAAGTCAACATGGATTATACCTTAACTATGTATTTCCAACAATACTGGAGAG ATAAAAGGCTCGCCTATTCTGGGATCCCTCTCAACCTCACGCTTGACAATCGAGTGGCTGACCAGCTCTG GGTGCCCGACACATATTTCTTAAATGACAAAAAGTCATTTGTCCACGGAGTGACAGTGAAAAACCGCATG ATCCGCCTCCACCCTGATGGGACAGTGCTGTATGGGCTCAGGATCACTACGACAGCAGCGTGCATGATGG ACCTCAGAAGATACCCACTGGATGAGCAAAACTGCACTTTGGAAATTGAAAGCTATGGCTACACTACGGA TGACATTGAATTTTACTGGCGTGGCGGGGACAAGGCTGTCACTGGCGTGGAAAGGATCGAGCTCCCACAG TTCTCCATTGTAGAGCACCGTCTGGTCTCCAGGAATGTTGTCTTCGCCACAGGTGCCTATCCTCGACTTT CATTGAGTTTTCGGTTGAAGAGAAATATCGGGTACTTCATTCTTCAGACGTATATGCCCTCAATCCTGAT CACAATCCTCTCGTGGGTGTCCTTCTGGATCAATTACGATGCATCTGCTGCTCGAGTTGCCCTTGGGATT ACCACCGTGCTCACCATGACAACCATCAACACTCACCTTCGGGAGACTCTACCCAAAATTCCCTATGTCA AAGCCATCGACATGTACCTGATGGGCTGCTTTGTCTTTGTATTCCTGGCACTTCTGGAGTACGCCTTTGT CAACTACATTTTCTTTGGAAGAGGTCCCCAAAGGCAGAAGAAGCTTGCGGAGAAGACAGCCAAGGCCAAG AATGATCGTTCTAAGAGTGAAATAAACCGGGTGGATGCTCACGGGAATATCCTATTAGCACCGATGGATG TTCACAATGAAATGAATGAGGTTGCAGGCAGCGTTGGTGACACCAGGAATTCAGCAATATCCTTTGACAA CTCAGGAATCCAGTATAGGAAACAGAGCATGCCCAAGGAAGGGCATGGGCGGTACATGGGAGACAGAAGC ATCCCGCACAAGAAGACCCACCTACGGAGGAGGTCTTCACAGCTCAAAATCAAAATCCCTGATCTAACCG ATGTGAATGCCATAGACAGATGGTCCCGCATCGTGTTTCCATTCACCTTTTCTCTCTTCAATTTAGTTTA CTGGCTGTACTATGTTAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001038701 |
Insert Size | 1422 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001038701.2, NP_001033790.1 |
RefSeq Size | 5516 bp |
RefSeq ORF | 1422 bp |
Locus ID | 14402 |
Gene Summary | Ligand-gated chloride channel which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:9108119). Plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA- gated ion channel (PubMed:27129275). The gamma2 subunit is necessary but not sufficient for a rapid formation of active synaptic contacts and the synaptogenic effect of this subunit is influenced by the type of alpha and beta subunits present in the receptor pentamer (PubMed:27129275). The alpha1/beta3/gamma2 receptor exhibits synatogenic activity whereas the alpha2/beta3/gamma2 receptor shows very little or no synaptogenic activity (PubMed:27129275). Functions also as histamine receptor and mediates cellular responses to histamine (By similarity). Plays an important role in somatosensation and in the production of antinociception (PubMed:10670447).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) contains an alternate exon at its 5' end and initiates translation at an alternate start codon compared to variant 1. The resulting protein (isoform b) is the same length but has a distinct N-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG222858 | Gabrb3 (tGFP-tagged) - Mouse gamma-aminobutyric acid (GABA) A receptor subunit beta 3 (Gabrb3) transcript variant 2, (10ug) |
CNY 3,710.00 |
|
MR222858 | Gabrb3 (Myc-DDK-tagged) - Mouse gamma-aminobutyric acid (GABA) A receptor, subunit beta 3 (Gabrb3), transcript variant 2 |
CNY 3,656.00 |
|
MR222858L3 | Lenti ORF clone of Gabrb3 (Myc-DDK-tagged) - Mouse gamma-aminobutyric acid (GABA) A receptor, subunit beta 3 (Gabrb3), transcript variant 2 |
CNY 5,230.00 |
|
MR222858L4 | Lenti ORF clone of Gabrb3 (mGFP-tagged) - Mouse gamma-aminobutyric acid (GABA) A receptor, subunit beta 3 (Gabrb3), transcript variant 2 |
CNY 6,056.00 |