Amy2b (NM_001190403) Mouse Untagged Clone

CAT#: MC216076

Amy2b (untagged) - Mouse amylase 2b (Amy2b), transcript variant 1, (10ug)



  "NM_001190403" in other vectors (4)

CNY 5,800.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Amy-X; Amy2-2; mAmy2-1; OTTMUSG00000022462
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC216076 representing NM_001190403
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGTTCGTTCTGCTGCTTTCCCTCATTGGGTTCTGCTGGGCTCAATATGACCCACATACTTCAGATG
GGAGGACTGCTATTGTCCACCTGTTCGAGTGGCGCTGGGTTGATATTGCCAAGGAATGTGAGCGATACTT
AGCTCCTAAGGGATTTGGAGGGGTGCAGGTCTCTCCACCCAATGAAAATATTGTAATTCATAACCCATCA
AGGCCTTGGTGGGAAAGATATCAACCAATCAGCTACAAAATTTGCACAAGGTCTGGAAATGAAGATGAAT
TCAGAGACATGGTGACAAGGTGCAACAATGTTGGTGTCCGTATTTATGTGGATGCTGTCATTAACCACAT
GTGTGGCTCAGGCAATCCTGCAGGAACAAGCAGTACCTGTGGAAGTTACCTCAATCCAAATAACAGGGAA
TTCCCAGCAGTTCCATACTCTGCTTGGGACTTTAACGATAATAAATGTAATGGAGAAATTAGTAACTACA
ATGATGCTTATCAGGTCAGAAATTGTCGTCTGTCTGGCCTTCTGGATCTTGCACTTGAGAAAGATTATGT
TCGTACCAAGGTTATTGATCTGGGTGGTGAGGCAATTAAAGGTAGTGAGTACTTTGGAAATGGCCGTGTA
ACAGAATTCAAGTTTGGTGCAAAACTTGGCACAGTTATCCGCAAGTGGAATGGAGAGAAGATGTCCTATT
TAAAGAACTGGGGAGAAGGTTGGGGTTTGGTGCCTTCTGACAGAGCCCTTGTGTTTGTGGACAACCATGA
CAATCAGCGAGGACATGGTGCTGGAGGATCATCCATCCTGACATTCTGGGATGCTAGAATGTACAAAATG
GCTGTTGGATTTATGTTGGCTCATCCTTATGGATTCACAAGAGTAATGTCAAGTTACCGTTGGAATAGAA
ATTTCCAGAATGGAAAAGATCAGAATGACTGGATTGGACCACCTAATAACAATGGAGTAACAAAAGAAGT
GACCATTAATGCAGACACTACTTGTGGCAATGACTGGGTCTGTGAACATAGATGGCGTCAAATAAGGAAC
ATGGTTGCCTTCAGGAATGTAGTCAATGGTCAGCCTTTTGCAAACTGGTGGGATAATAACAGCAACCAAG
TAGCTTTTAGCAGAGGAAACAGAGGCTTCATTGTCTTTAACAATGATGACTGGGCTTTGTCAGCCACTTT
ACAGACTGGTCTTCCTGCTGGCACATACTGTGATGTCATCTCTGGAGATAAGGTCGATGGCAATTGCACT
GGACTTAAAGTAAATGTTGGCAGTGATGGTAAAGCTCACTTTTCCATTAGTAACTCTGCTGAGGACCCAT
TTATTGCAATCCATGCTGACTCAAAACTGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001190403
Insert Size 1362 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001190403.1, NP_001177332.1
RefSeq Size 1413 bp
RefSeq ORF 1362 bp
Locus ID 545562
Gene Summary Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The mouse genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. At least one mouse strain has a non-functional allele of this gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2011]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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