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Cdc14b (NM_001122989) Mouse Untagged Clone
CAT＃: MC216040
Cdc14b (untagged) - Mouse CDC14 cell division cycle 14 homolog B (S. cerevisiae) (Cdc14b), transcript variant 2, (10ug)
CNY 5,800.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2810432N10Rik; A530086E13Rik; AA472821; Cdc14B1; CDC14B3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC216040 representing NM_001122989
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGACGGGAGGGCGCAGGGACCCCTCTGATGGCCGAGGTGATCCGAGATCGCCTTTGTTTTGCCATTC TCTACAGCAGACCAAAGAGTGCAACAAACGAACATTATTTCAGCATAGATAACGAGCTAGAGTATGAGAA CTTCTATGCGGATTTTGGACCACTCAATCTGGCCATGGTTTACAGATATTGTTGCAAGATAAATAAGAAG CTCAAGTCCATTACAATGCTGAGGAAGAAAATTATCCATTTTACTGGCACTGATCAGAGAAAGCAAGCGA ATGCCGCCTTCCTTGTCGGGTGTTATATGGTTATATATTTGGGGAGGACACCAGAAGACGCGTATAGAAC ATTAATCTTCGGAGATACAGCCTATATTCCTTTCAGAGATGCTGCCTATGGAAGCTGCAGTTTCTATATT ACACTTCTCGACTGTTTTCACGCAGTAAAGAAGGCAATGCAGTATGGCTTCTTTAATTTCAACTCATTTA ACCTTGACGAATATGAACACTATGAAAAAGCAGAAAATGGAGATTTCAACTGGATAATACCAGAGCGATT TCTTGCCTTCTGTGGGCCCCATTCAAGATCCAGGCTGGAAAGTGGTTACCACCAACATTCTCCGGAGACT TATATTCCATATTTTAAGAATCACAATGTAACTACCATTATTCGTCTGAATAAAAGGATGTATGATGCTA AGCGCTTTACGGATGCTGGATTCGATCACCATGATCTTTTCTTTCCGGATGGCAGCACCCCTGCTGAGTC AATTGTCCAAGAATTTCTGGATATTTGTGAAAATGTCAAGGGTGCCATTGCTGTGCATTGCAAAGCTGGC CTTGGTCGAACAGGCACTCTGATAGGCTGCTACCTCATGAAGCATTACAGGATGACAGCAGCTGAGAGCA TTGCCTGGCTCAGAATCTGTAGACCCGGCTCAGTAATTGGGCCCCAGCAACAGTTTCTGGTCATGAAACA ATCAAGCCTCTGGCTAGAAGGCGACTATTTCCGTCAAAAGTTAAGAGGGCAGGAGAATGGCCCCCTCAGA GAAGCCTTCTCCAAACACCTTTCGGATGCTGATGACCTGTCCTTAAATGGGCTTGAGAATCAAGACAATC AAGAGCCTGAGCCGTATAGTGATGATGATGAAGTCAGTGGAATGACACAAGGAGACAGACTTCGGGCCCT GAAAAGCCGGAGACAGCCGAAAGCCAGCGCTATCCCCCTCACATGTCCCCTAGCTGTGCTGACCTCTGCA CTGTGTAGTGTTGCCATCTGGTGGATTGTGTGTGACTACATTCTTCCCACCCTGCTGTTCTGTCTTGATG GCTTCAGAACACAGTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_001122989 |
| Insert Size | 1347 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001122989.1, NP_001116461.1 |
| RefSeq Size | 5595 bp |
| RefSeq ORF | 1347 bp |
| Locus ID | 218294 |
| UniProt ID | Q6PFY9 |
| Gene Summary | Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylates SIRT2 around early anaphase. Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, compared to variant 1, resulting in the use of a novel start codon and a protein (isoform 2) with a shorter, distinct N-terminus, compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG218991 | Cdc14b (tGFP-tagged) - Mouse CDC14 cell division cycle 14 homolog B (S. cerevisiae) (Cdc14b) transcript variant 2, (10ug) |
CNY 4,940.00 |
|
| MR218991 | Cdc14b (Myc-DDK-tagged) - Mouse CDC14 cell division cycle 14 homolog B (S. cerevisiae) (Cdc14b), transcript variant 2 |
CNY 4,470.00 |
|
| MR218991L3 | Lenti ORF clone of Cdc14b (Myc-DDK-tagged) - Mouse CDC14 cell division cycle 14 homolog B (S. cerevisiae) (Cdc14b), transcript variant 2 |
CNY 6,370.00 |
|
| MR218991L4 | Lenti ORF clone of Cdc14b (mGFP-tagged) - Mouse CDC14 cell division cycle 14 homolog B (S. cerevisiae) (Cdc14b), transcript variant 2 |
CNY 6,370.00 |
