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Glra2 (NM_183427) Mouse Untagged Clone
CAT＃: MC215910
Glra2 (untagged) - Mouse glycine receptor, alpha 2 subunit (Glra2), (10ug)
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC215910 representing NM_183427
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACCGGCAGCTAGTGAACATTTTGACAGCCTTGTTTGCATTTTTCTTAGGGACAAACCACTTCAGGG AAGCTTTCTGCAAAGACCATGACTCCAGGTCTGGAAAACATCCCTCGCAGACCCTATCTCCTTCAGATTT CTTGGATAAATTAATGGGAAGAACATCAGGATATGATGCAAGAATCAGGCCAAATTTTAAAGGTCCTCCA GTAAACGTTACTTGCAATATTTTTATCAACAGTTTTGGATCAGTCACAGAAACCACCATGGACTACCGAG TGAACATTTTTCTGAGACAGCAGTGGAATGATTCACGGCTGGCATACAGTGAGTACCCAGATGATTCCCT GGATTTGGATCCCTCAATGTTGGATTCGATTTGGAAACCGGATTTGTTCTTTGCCAATGAAAAGGGTGCC AATTTCCATGATGTCACCACTGACAACAAGTTGTTGCGGATTTCCAAAAATGGCAAAGTGCTCTACAGTA TTAGACTCACCTTGACTTTATCTTGTCCAATGGACCTGAAGAACTTTCCAATGGATGTCCAGACCTGTAC AATGCAGCTGGAGAGTTTTGGGTACACCATGAATGACCTGATATTTGAGTGGTTAAGTGATGGTCCAGTA CAAGTTGCTGAAGGACTCACCTTGCCCCAGTTTATTTTGAAAGAAGAGAAGGAGCTTGGTTATTGCACAA AGCATTACAACACTGGCAAGTTTACCTGCATTGAGGTCAAGTTTCACCTGGAGCGCCAGATGGGCTACTA TTTGATCCAGATGTACATCCCCAGCCTGTTGATAGTCATTTTGTCCTGGGTCTCCTTTTGGATAAACATG GATGCAGCCCCTGCCAGGGTTGCCCTGGGCATCACAACAGTCCTGACAATGACTACACAGAGTTCAGGTT CCAGGGCATCTCTGCCAAAGGTCTCCTATGTGAAAGCAATTGACATCTGGATGGCGGTATGCCTTCTCTT TGTGTTTGCTGCCTTACTGGAATATGCAGCAGTGAACTTTGTCTCCAGGCAACATAAGGAGTTCCTTCGT CTCCGGAGACGACAGAAGAGGCAGAATAAGGAAGAAGATGTTACTCGTGAAAGTCGTTTTAACTTCAGTG GCTATGGGATGGGTCACTGCCTCCAAATGAAAGATGGCACAGCTGTCAAGGCTACACCTGCCAACCCACT TCCACAACCCCCCAAAGATGCAGATGCTATCAAGAAGAAGTTTGTGGATCGGGCAAAAAGAATTGACACC ATATCTCGAGCTGCCTTCCCACTGGCCTTCCTCATTTTCAACATCTTTTACTGGATCACATACAAGATCA TTCGGCATGAAGATGTCCACAAGAAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_183427 |
| Insert Size | 1359 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC068987, AAH68987 |
| RefSeq Size | 2775 bp |
| RefSeq ORF | 1359 bp |
| Locus ID | 237213 |
| UniProt ID | Q7TNC8 |
| Gene Summary | Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine. Channel opening is also triggered by taurine and beta-alanine. Plays a role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents. Plays a role in cellular responses to ethanol.[UniProtKB/Swiss-Prot Function] Transcript Variant: Variants 1 and 2 encode the same isoform (a). Isoforms and b are the same length but differ in their sequence. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG207214 | Glra2 (tGFP-tagged) - Mouse glycine receptor, alpha 2 subunit (Glra2) |
CNY 5,040.00 |
|
| MR207214 | Glra2 (Myc-DDK-tagged) - Mouse glycine receptor, alpha 2 subunit (Glra2) |
CNY 5,488.00 |
|
| MR207214L3 | Lenti ORF clone of Glra2 (Myc-DDK-tagged) - Mouse glycine receptor, alpha 2 subunit (Glra2) |
CNY 6,460.00 |
|
| MR207214L4 | Lenti ORF clone of Glra2 (mGFP-tagged) - Mouse glycine receptor, alpha 2 subunit (Glra2) |
CNY 6,460.00 |
