Gcnt3 (NM_028087) Mouse Untagged Clone

CAT#: MC215765

Gcnt3 (untagged) - Mouse glucosaminyl (N-acetyl) transferase 3, mucin type (Gcnt3), (10ug)



  "NM_028087" in other vectors (3)

CNY 5,700.00


货期*
5周

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2010013H22Rik; 2210021I22Rik; 2210401J11Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC215765 representing NM_028087
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTTCCTGGCAGAGGCTCTGCTGGCACTATCGCCTGTGGACCCTGGGTTGCTACATGCTACTGGCCA
TCCTTGCCCTGAAACTGTCCCTCAGACTGAAGTGTGACTTCGATGCCATGGATCTGGACTCTGAGGAATT
TCAAAGCCAGTACTGCAGGGATCTCCTGTACAAGACCCTGAAGCTGCCAGCCAAGAGTTCCATCAACTGC
TCAGGGGTCATTCGAGGGGAGCAGAAAGCGGTGACCCAGGCTCTGCTGAATAACCTGGAAATTAAGAAGA
AGCAGCAGCTCTTCACAGAGGCCGACTACCTTAGGATGACAGCAGACTGTGAGCACTTCAAGACCAAGAG
GAAGTTTATACAGGTCCCACTGAGCAAGGAAGAGGCCAGCTTCCCCATTGCGTACTCCATGGTGGTGCAT
GAGAAGATTGAGAACTTCGAAAGGTTGCTGCGAGCTGTGTACACCCCTCAGAATGTATACTGTGTCCACA
TGGATCAGAAGTCTTCAGAACCCTTTAAGCAGGCAGTCAGGGCCATCGTGTCATGCTTCCCCAATGTCTT
CATAGCTAGTAAGTTGGTGTCAGTGGTCTATGCTTCCTGGTCCAGGGTGCAGGCTGACCTAAACTGCATG
GAAGACTTGCTTCAGAGCCCCGTGCCATGGAAATACCTCCTGAACACCTGTGGGACAGACTTTCCCATCA
AAACCAATGCTGAGATGGTCAAGGCCCTCAAGCTATTGAAAGGGCAGAACAGTATGGAGTCAGAGGTACC
ACCTCCACATAAAAAATCCCGCTGGAAATATCACTATGAGGTGACAGACACATTGCACATGACCAGCAAG
AGGAAGACGCCGCCACCTAATAACCTAACCATGTTCACTGGGAATGCCTACATGGTGGCTTCTCGAGACT
TCATTGAACACGTGTTCAGTAACTCAAAAGCCCGGCAACTGATCGAGTGGGTAAAAGACACCTATAGTCC
CGATGAGCACCTTTGGGCCACCCTCCAGCGTGCCTCGTGGATGCCTGGATCAGATCCCTTGCATCGAAAA
TTTGACCTGTCAGACATGAGAGCCATTGCGAGACTAACCAAGTGGTACGACCATGAGGGAGACATTGAGA
ACGGGGCACCTTACACGTCTTGCTCAGGAATCCACCAGCGGGCTGTCTGTGTTTATGGGTCAGGGGACCT
GCACTGGATACTTCAGAACCATCACCTCTTGGCCAACAAGTTTGACCCAAAGGTGGATGATAATGTTCTT
CAGTGTTTAGAAGAATATTTACGTCACAAAGCCATCTATGGGACTGAACTATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_028087
Insert Size 1314 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_028087.2, NP_082363.2
RefSeq Size 4301 bp
RefSeq ORF 1314 bp
Locus ID 72077
UniProt ID Q5JCT0
Gene Summary Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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