Opn5 (NM_181753) Mouse Untagged Clone

CAT#: MC214652

Opn5 (untagged) - Mouse opsin 5 (Opn5), (10ug)



  "NM_181753" in other vectors (4)

CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Gpr136; Neuropsin; PGR12; TMEM13
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC214652 representing NM_181753
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTTGAACCACACTGCCCTACCTCAGGATGAGCGCCTGCCCCACTATCTTCGAGACGAGGACCCTT
TTGCTTCCAAACTTTCCTGGGAAGCGGATTTAGTGGCTGGCTTTTACCTAACAATAATCGGGATTCTCTC
TACATTTGGAAATGGGTATGTCCTTTATATGTCTTCTAGACGCAAGAAGAAGCTGAGACCTGCGGAAATA
ATGACTATCAATTTAGCAGTCTGTGATCTGGGGATATCAGTTGTAGGCAAGCCGTTCACCATCATCTCTT
GCTTCTGCCACCGCTGGGTGTTTGGCTGGTTTGGCTGCCGCTGGTATGGCTGGGCTGGATTTTTCTTTGG
CTGTGGAAGCCTGATTACCATGACTGCTGTCAGCCTGGACCGCTATCTGAAGATCTGTTATCTGTCTTAT
GGGGTCTGGCTGAAGAGAAAGCATGCCTACATCTGCCTGGCAGTCATCTGGGCTTATGCTTCCTTCTGGA
CCACCATGCCCTTGGTGGGCCTGGGGGACTATGCACCTGAGCCCTTCGGAACCTCATGCACCCTGGACTG
GTGGCTGGCCCAGGCTTCAGGTGGGGGTCAGGTGTTCATCCTGAGCATCCTCTTCTTCTGCCTCCTGCTG
CCAACGGCTGTGATTGTTTTCTCATATGCTAAGATCATCGCCAAGGTGAAGTCTTCTTCTAAAGAGGTAG
CCCATTTCGACAGTCGAATCCATAGCAGCCATGTACTTGAGGTGAAGCTGACCAAGGTGGCAATGCTGAT
TTGTGCTGGTTTCCTGATTGCCTGGATTCCTTATGCGGTCGTCTCTGTGTGGTCAGCTTTTGGAAGGCCA
GACTCCATCCCCATACAGCTCTCCGTGGTGCCCACACTCCTTGCAAAATCAGCAGCGATGTACAATCCAA
TCATCTACCAGGTCATTGATTACAGATTTGCCTGTTGCCAGGCTGGTGGTTTGAGAGGAACGAAGAAGAA
ATCTTTGGAAGACTTTAGGCTGCATACTGTAACCGCAGTCAGGAAGTCTTCTGCTGTGCTGGAGATCCAT
CCAGAGAGCAGTTCCAGATTTACTAGTGCCCATGTTATGGATGGAGAGAGTCACAGTAATGATGGTGACT
GTGGCAAGAAATAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_181753
Insert Size 1134 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_181753.4, NP_861418.2
RefSeq Size 1824 bp
RefSeq ORF 1134 bp
Locus ID 353344
UniProt ID Q6VZZ7
Gene Summary G-protein coupled receptor which selectively activates G(i) type G proteins via ultraviolet A (UVA) light-mediated activation in the retina (PubMed:22043319). Preferentially binds the chromophore 11-cis retinal and is a bistable protein that displays emission peaks at 380 nm (UVA light) and 470 nm (blue light) (PubMed:22043319, PubMed:31607531). Required for the light-response in the inner plexiform layer, and contributes to the regulation of the light-response in the nerve fiber layer, via phosphorylated DAT/SLC6A3 dopamine uptake (PubMed:30936473). Involved in local corneal and retinal circadian rhythm photoentrainment via modulation of the UVA light-induced phase-shift of the retina clock (PubMed:26392540, PubMed:30240620). Acts as a circadian photoreceptor in the outer ear and vibrissal pads, via modulation of circadian clock-gene expression in response to violet light during the light-to-dark transition phase and night phase of the circadian cycle (PubMed:31607531). Required in the retina to negatively regulate hyaloid vessel regression during postnatal development via light-dependent OPN5-SLC32A1-DRD2-VEGFR2 signaling (PubMed:30936473). Involved in the light-dependent regulation of retina and vitreous compartment dopamine levels (PubMed:30936473).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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