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Dusp7 (NM_153459) Mouse Untagged Clone
CAT＃: MC213009
Dusp7 (untagged) - Mouse dual specificity phosphatase 7 (Dusp7), (10ug)
CNY 3,656.00
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AU015694; Mkpx; Pyst2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC213009 representing NM_153459
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAAACCAGCTCCGCGGGCCCCCAGTGCGGGCGCACATGTCGACTTCGGGGGCGGCGGCGGCGGCGG CGGCTGGGGGCACTCGGGCGGGGTCGGAGCCCGGTGCGGGATCTGGGTCCGGCGCAGGCATCGGAGCGGG AGCGACGACGGGAGCGGGGGCCATGCCCTGCAAGAGCGCCGAGTGGCTGCAGGAGGAGCTGGAGGCGCGC GGTGGCGCGTCCCTGCTGCTGCTAGATTGCCGGCCGCACGAGCTCTTCGAATCGTCGCACATTGAGACGG CCATCAACCTGGCCATCCCGGGTCTCATGCTGCGCCGCCTGCGCAAAGGCAACCTGCCCATCCGCTCCAT CATTCCCAACCATGCCGACAAGGAGCGCTTCGCCACGCGCTGCAAGGCGGCCACCGTGCTGCTCTACGAC GAGGCCACGGCGGAGTGGCAGCCCGAGCCCGGCGCCCCCGCCTCCGTTCTCGGCCTGCTCCTGCAGAAGC TGCGAGACGACGGCTGCCAGGCCTACTACCTCCAAGGTGGTTTCAACAAGTTCCAGACGGAATACTCCGA GCATTGTGAGACTAACGTGGACAGCTCGTCCTCCCCGAGTGGCTCGCCACCCACCTCCGTGCTGGGCCTG GGGGGCCTGCGCATCAGCTCTGACTGCTCAGACGGCGAGTCGGACCGAGAGCTGCCCAGCAGTGCCACTG AGTCGGATGGCAGCCCTGTACCATCCAGCCAGCCGGCCTTTCCAGTCCAGATCCTGCCCTACCTCTACCT CGGCTGTGCCAAGGACTCTACCAACCTGGATGTACTTGGCAAATACGGCATCAAATACATCCTCAACGTC ACACCCAACCTGCCCAACGCCTTTGAGCACGGTGGTGAGTTCACCTACAAGCAAATCCCCATCTCTGACC ACTGGAGCCAGAACCTCTCTCAGTTCTTCCCCGAGGCCATCAGCTTCATTGATGAAGCCCGCTCCAAGAA GTGTGGTGTCTTGGTGCACTGCCTAGCAGGCATCAGCCGTTCAGTGACAGTTACTGTAGCCTACCTGATG CAGAAGATGAACCTGTCCCTCAACGACGCCTATGACTTCGTCAAGAGGAAAAAGTCTAACATCTCGCCCA ACTTCAACTTCATGGGGCAGCTGCTGGACTTTGAGCGGACGCTGGGGCTCAGCAGCCCATGCGACAACCA CGCACCCAGTGAGCAGCTCTACTTCTCCACGCCCACCAACCATAACCTGTTCCCGATCAACACGCTCGAG TCCACGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_153459 |
| Insert Size | 1269 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_153459.4, NP_703189.3 |
| RefSeq Size | 3227 bp |
| RefSeq ORF | 1269 bp |
| Locus ID | 235584 |
| UniProt ID | Q91Z46 |
| Gene Summary | Dual specificity protein phosphatase (PubMed:27783954). Shows high activity towards MAPK1/ERK2 (By similarity). Also has lower activity towards MAPK14 and MAPK8 (By similarity). In arrested oocytes, plays a role in meiotic resumption (PubMed:27783954). Promotes nuclear envelope breakdown and activation of the CDK1/Cyclin-B complex in oocytes, probably by dephosphorylating and inactivating the conventional protein kinase C (cPKC) isozyme PRKCB (PubMed:27783954). May also inactivate PRKCA and/or PRKCG (PubMed:27783954). Also important in oocytes for normal chromosome alignment on the metaphase plate and progression to anaphase, where it might regulate activity of the spindle-assembly checkpoint (SAC) complex (PubMed:27783954).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG215943 | Dusp7 (tGFP-tagged) - Mouse dual specificity phosphatase 7 (Dusp7), (10ug) |
CNY 3,710.00 |
|
| MR215943 | Dusp7 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 7 (Dusp7) |
CNY 3,656.00 |
|
| MR215943L3 | Lenti ORF clone of Dusp7 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 7 (Dusp7) |
CNY 5,230.00 |
|
| MR215943L4 | Lenti ORF clone of Dusp7 (mGFP-tagged) - Mouse dual specificity phosphatase 7 (Dusp7) |
CNY 5,230.00 |
