Arfgap1 (NM_001177710) Mouse Untagged Clone

CAT#: MC212845

Arfgap1 (untagged) - Mouse ADP-ribosylation factor GTPase activating protein 1 (Arfgap1), transcript variant 6, (10ug)



  "NM_001177710" in other vectors (4)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI115377; Arf1gap
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC212845 representing NM_001177710
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAATAATCTTTGTGCGCTCTGTTACAATGGACAAATGGAAGGACATTGAGCTGGAGAAGATGAAGG
CTGGTGGGAATGCTAAGTTCCGAGAGTTCCTGGAGACACAGGACGACTATGAGCCTAGCTGGTCACTGCA
GGACAAGTACAGCAGCAGAGCCGCGGCGCTCTTCAGGGATAAGGTGGCTACTTTGGCAGAAGGTAAAGAG
TGGTCTCTGGAGTCATCGCCTGCACAGAACTGGACCCCACCTCAGCCCAAGACACTGCAGTTCACTGCCC
ACCGAGCCTCTGGCCAGCCACAGAGTGCAGCCGCCTCTGGGGACAAGGCTTTTGAAGATTGGCTGAATGA
TGACCTGGGTTCCTACCAGGGTGCTCAGGAGAATCGCTATGTAGGGTTTGGGAACACAGTGCCACCTCAG
AAGAGAGAAGATGACTTCCTCAACAATGCCATGTCATCGCTGTACTCGGGCTGGAGCAGTTTTACCACTG
GGGCGAGCAAGTTTGCATCTGCAGCAAAGGAGGGTGCTACAAAATTTGGATCTCAAGCAAGTCAGAAGGC
TTCGGAGTTGGGCCACAGCCTGAATGAGAATGTTCTCAAGCCTGCACAGGAGAAGGTGAAGGAGGGAAGG
ATTTTTGATGATGTGTCCAGTGGGGTCTCTCAGTTGGCATCCAAGGTCCAGGGAGTTGGCAGTAAGGGAT
GGCGTGATGTCACTACTTTCTTCTCTGGGAAAGCCGAAGACTCTTCAGACAGACCCTTAGAGGGCCACAG
CTACCAGAACAGCAGTGGAGACAACTCTCAGAACAGCAACATAGACCAGAGCTTCTGGGAGACCTTTGGG
AGTGCTGAGCCCCCCAAGGCCAAGTCCCCAAGCAGTGACAGCTGGACCTGTGCAGATGCTTCAACAGGGA
GGAGGAGCTCGGACAGCTGGGACGTTTGGGGCTCAGGTTCCGCATCCAACAACAAGAACAGCAATAGCGA
TGGCTGGGAGAGTTGGGAGGGAGCCAGTGGGGAGGGCAGGGCAAAGGCCACCAAGAAGGCAGCCCCATCC
ACGGCTGATGAGGGCTGGGACAACCAGAACTGGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001177710
Insert Size 1086 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001177710.1, NP_001171181.1
RefSeq Size 2398 bp
RefSeq ORF 1086 bp
Locus ID 228998
Gene Summary GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (6) differs in the 5' UTR and lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The resulting isoform (d) lacks an internal segment near the N-terminus, compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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