Brdt (NM_001079873) Mouse Untagged Clone

CAT#: MC212274

Brdt (untagged) - Mouse bromodomain, testis-specific (Brdt), transcript variant 2, (10ug)



  "NM_001079873" in other vectors (4)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 7420412D09Rik; Brd; Brd6; Fsr; Fsrg3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC212274 representing NM_001079873
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCTGCCAAGTCGACAAACAGCTATTGTTAACCCCCCTCCACCAGAGTACATAAACACTAAGAAGA
GTGGGCGGTTGACGAATCAGCTGCAGTTCCTACAGAGGGTTGTGCTGAAGGCCCTGTGGAAGCACGGCTT
CTCTTGGCCTTTCCAACAGCCGGTGGACGCCGTGAAACTAAAGCTGCCTGACTATTACACCATCATAAAA
ACCCCAATGGATTTAAATACAATTAAGAAGCGGTTGGAAAATAAGTATTATGAGAAGGCTTCCGAATGCA
TAGAAGACTTCAACACAATGTTCTCAAACTGCTACTTATACAACAAGACTGGAGATGACATTGTTGTTAT
GGCCCAAGCTCTAGAGAAGTTATTTATGCAGAAATTATCTCAGATGCCACAAGAAGAGCAAGTTGTGGGT
GGTAAGGAGAGAATAAAGAAAGATATCCAACAAAAGATAGCGGTTTCCTCTGCTAAGGAACAAATCCCCT
CCAAAGCGGCAGAGAATGTCTTTAAGCGGCAAGAGATTCCTTCTGGGCTTCCTGACATCTCTCTCTCGCC
CTTAAACATGGCACAAGAAGCTCCCCCGATCTGTGACTCGCAGTCTCTGGTCCAAATTACAAAGGGTGTG
AAGAGGCGAGCAGACACAACAACTCCTACCACTTCCATAGCTAAAGCAAGTAGCGAATCTCCTCCGACAC
TTAGAGAAACAAAGCCAGTGAACATGCCAGTAAAAGAAAATACAGTAAAGAACGTTTTGCCAGACTCTCA
GCAACAACACAAAGTTTTAAAAACAGTCAAAGTAACGGAGCAATTAAAGCACTGCAGCGAGATTCTTAAA
GAAATGCTTGCCAAGAAACATTTGCCGTATGCATGGCCCTTCTATAATCCTGTGGATGCTGATGCTTTGG
GACTCCACAACTACTATGACGTTGTCAAAAATCCCATGGATCTCGGAACGATCAAGGTAAATACTGCCTG
A


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001079873
Insert Size 981 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001079873.1, NP_001073342.1
RefSeq Size 1588 bp
RefSeq ORF 981 bp
Locus ID 114642
UniProt ID Q91Y44
Gene Summary The protein encoded by this gene belongs to the BET protein family. BET proteins have two N-terminal bromodomains and one C-terminal extraterminal domain (ET domain). BET proteins regulate chromatin reorganization via binding to acetylated histones. This gene is thought to play a role in the transcriptional regulation of spermatogenesis. Although referred to as testis-specific bromodomain (Brdt) protein, RT-PCR indicates that this gene is expressed in both mouse oocytes and testes. Alternative splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform B) that lacks the 3' bromodomain and ET domain, compared to isoform A.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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