Brdt (NM_001079873) Mouse Untagged Clone
CAT#: MC212274
Brdt (untagged) - Mouse bromodomain, testis-specific (Brdt), transcript variant 2, (10ug)
CNY 3,990.00
Product images
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Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 7420412D09Rik; Brd; Brd6; Fsr; Fsrg3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC212274 representing NM_001079873
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCTGCCAAGTCGACAAACAGCTATTGTTAACCCCCCTCCACCAGAGTACATAAACACTAAGAAGA GTGGGCGGTTGACGAATCAGCTGCAGTTCCTACAGAGGGTTGTGCTGAAGGCCCTGTGGAAGCACGGCTT CTCTTGGCCTTTCCAACAGCCGGTGGACGCCGTGAAACTAAAGCTGCCTGACTATTACACCATCATAAAA ACCCCAATGGATTTAAATACAATTAAGAAGCGGTTGGAAAATAAGTATTATGAGAAGGCTTCCGAATGCA TAGAAGACTTCAACACAATGTTCTCAAACTGCTACTTATACAACAAGACTGGAGATGACATTGTTGTTAT GGCCCAAGCTCTAGAGAAGTTATTTATGCAGAAATTATCTCAGATGCCACAAGAAGAGCAAGTTGTGGGT GGTAAGGAGAGAATAAAGAAAGATATCCAACAAAAGATAGCGGTTTCCTCTGCTAAGGAACAAATCCCCT CCAAAGCGGCAGAGAATGTCTTTAAGCGGCAAGAGATTCCTTCTGGGCTTCCTGACATCTCTCTCTCGCC CTTAAACATGGCACAAGAAGCTCCCCCGATCTGTGACTCGCAGTCTCTGGTCCAAATTACAAAGGGTGTG AAGAGGCGAGCAGACACAACAACTCCTACCACTTCCATAGCTAAAGCAAGTAGCGAATCTCCTCCGACAC TTAGAGAAACAAAGCCAGTGAACATGCCAGTAAAAGAAAATACAGTAAAGAACGTTTTGCCAGACTCTCA GCAACAACACAAAGTTTTAAAAACAGTCAAAGTAACGGAGCAATTAAAGCACTGCAGCGAGATTCTTAAA GAAATGCTTGCCAAGAAACATTTGCCGTATGCATGGCCCTTCTATAATCCTGTGGATGCTGATGCTTTGG GACTCCACAACTACTATGACGTTGTCAAAAATCCCATGGATCTCGGAACGATCAAGGTAAATACTGCCTG A ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001079873 |
Insert Size | 981 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001079873.1, NP_001073342.1 |
RefSeq Size | 1588 bp |
RefSeq ORF | 981 bp |
Locus ID | 114642 |
UniProt ID | Q91Y44 |
Gene Summary | The protein encoded by this gene belongs to the BET protein family. BET proteins have two N-terminal bromodomains and one C-terminal extraterminal domain (ET domain). BET proteins regulate chromatin reorganization via binding to acetylated histones. This gene is thought to play a role in the transcriptional regulation of spermatogenesis. Although referred to as testis-specific bromodomain (Brdt) protein, RT-PCR indicates that this gene is expressed in both mouse oocytes and testes. Alternative splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform B) that lacks the 3' bromodomain and ET domain, compared to isoform A. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG224116 | Brdt (tGFP-tagged) - Mouse bromodomain testis-specific (Brdt) transcript variant 2, (10ug) |
CNY 2,850.00 |
|
MR224116 | Brdt (Myc-DDK-tagged) - Mouse bromodomain, testis-specific (Brdt), transcript variant 2 |
CNY 2,400.00 |
|
MR224116L3 | Lenti ORF clone of Brdt (Myc-DDK-tagged) - Mouse bromodomain, testis-specific (Brdt), transcript variant 2 |
CNY 4,750.00 |
|
MR224116L4 | Lenti ORF clone of Brdt (mGFP-tagged) - Mouse bromodomain, testis-specific (Brdt), transcript variant 2 |
CNY 4,750.00 |