Ift57 (NM_028680) Mouse Untagged Clone

CAT#: MC211472

Ift57 (untagged) - Mouse intraflagellar transport 57 homolog (Chlamydomonas) (Ift57), (10ug)



  "NM_028680" in other vectors (4)

CNY 3,990.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 4833420A15Rik; Esrrbl1; Hippi; MHS4R2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC211472 representing NM_028680
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCCGCGGCCGCGGTGATCCCGCCGTCGGGCTTGGACGATGGGGTGTCTCGGGCTCGCGGGGAAG
GCGCAGGGGAGGCTGTGGTGGAGCGCGGGCCAGGAGCGGCCTACCACATGTTCGTGGTGATGGAAGACTT
AGTGGAGAAGCTGAAGCTGCTCCGCTACGAGGAGGAGCTACTCCGAAAGAGCAATCTGAAGCCCCCGTCC
AGACACTACTTTGCTCTGCCTACCAACCCAGGCGAGCAGTTCTACATGTTTTGCACTCTTGCTGCGTGGC
TGATCAACAAAACTGGCCGTGCCTTTGAGCAGCCTCAAGAATACGACGATCCCAATGCAACTATATCTAA
TATACTCTCTGAGCTTCGCTCTTTTGGGAGAACTGCAGATTTTCCTCCTTCAAAATTAAAGTCTGGTTAC
GGAGAACAAGTGTGCTATGTTCTTGATTGCTTAGCTGAAGAAGCTTTAAAATATATTGGTTTCACTTGGA
AAAGGCCATCATACCCAGTGGAAGAACTAGAAGAAGAAACTGTTCCAGAAGATGATGCCGAGTTAACATT
AAGTAAAGTGGATGAAGAATTTGTGGAAGAGGAGACAGATAATGAAGAAAACTTTATTGATCTCAACGTT
TTAAAGGCCCAGACCTATCGCTTGGACACAAACGAGTCTGCCAAACAAGAAGATATTTTGGAATCTACGA
CAGATGCTGCGGAATGGAGCCTAGAAGTTGAGCGTGTACTACCGCAGCTGAAAGTCACGATTAGGACTGA
CAATAAGGATTGGAGGATCCATGTTGACCAAATGCACCAGCACAAAAGTGGGATTGAATCTGCTCTGAAG
GAGACCAAGGGGTTTTTGGACAAGCTCCATAATGAAATTAGCAGGACTCTGGAAAAGATTGGCAGCCGAG
AAAAGTACATTAACAATCAACTTGAGCACTTGGTTCAAGAATATCGTGGGGCCCAAGCCCAGCTAAGTGA
GGCAAGGGAGCGCTACCAGCAGGGCAATGGCGGAGTAACTGAACGGACCAGACTCCTCTCTGAGGTTACA
GAAGAATTAGAAAAGGTAAAGCAAGAAATGGAAGAGAAGGGCAGCAGCATGACGGACGGCACTCCTTTGG
TGAAGATTAAGCAGAGCTTAACCAAGCTGAAGCAAGAAACTGTTCAGATGGACATTAGAATCGGTGTGGT
GGAGCACACGCTACTTCAGTCAAAACTCAAGGAGAAGTGCAACATGACCAGGGACATGCATGCAGCTGTC
ACCCCAGAGTCAGCAATTGGCTTCTATTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_028680
Insert Size 1290 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_028680.3, NP_082956.2
RefSeq Size 2540 bp
RefSeq ORF 1290 bp
Locus ID 73916
UniProt ID Q8BXG3
Gene Summary Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway. Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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