Dcp2 (NM_027490) Mouse Untagged Clone

CAT#: MC211089

Dcp2 (untagged) - Mouse DCP2 decapping enzyme homolog (S. cerevisiae) (Dcp2), (10ug)



  "NM_027490" in other vectors (4)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2410015D23Rik; 5730537H01Rik; AL118268
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC211089 representing NM_027490
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCCAAACGCCTGGAGATTCCCGGCAGCGTCCTGGATGATCTCTGCAGCCGATTTATTTTGCATA
TTCCCAGTGAAGAAAGAGACAATGCGATCCGAGTGTGCTTCCAGATTGAACTTGCCCATTGGTTTTACTT
GGATTTCTACATGCAGAACACACCAGGATTACCTCAGTGCGGGATAAGAGACTTTGCCAAAGCTGTTTTC
AGTCACTGTCCATTTCTGCTGCCACAAGGTGAAGATGTGGAAAAGATTTTGGATGAATGGAAGGAATATA
AAATGGGAGTCCCGACTTACGGTGCAATTATTCTCGATGAGACACTGGAGAATGTACTGCTGGTTCAGGG
GTACCTGGCAAAGTCAGGCTGGGGATTCCCAAAAGGAAAAGTAAATAAAGAAGAGGCGCCTCATGACTGT
GCGGCGAGAGAGGTCTTCGAGGAAACTGGTTTTGATATAAAAGACTATATTTGTAAGGATGATTACATTG
AACTGCGAATCAATGACCAGCTTGCTCGCTTGTACATCATTCCAGGAGTCCCAAAAGACACAAAATTTAA
CCCCAAAACCAGAAGAGAAATTAGGAATATTGAGTGGTTCTCCATTGAGAAATTGCCCTGTCATAGAAAT
GACATGACTCCCAAGTCCAAGCTTGGCTTGGCACCTAATAAGTTTTTTATGGCCATTCCCTTTATCAGAC
CACTAAGGGACTGGCTGTCTCGAAGATTTGGAGATTCCTCGGACAGTGACAATGGGTTTTCCTCAGCTGG
TAGCACACCAGCTAGACCCACCGTGGAGAAATTGAGTCGAACAAAATTCCGCCACAGCCAGCAGCTGTTT
CCTGAAGGTTCCCCGAGTGACCAGTGGGTTAAGCACCGGCAGCCACTGCAGCAGAAGTCACACAGTAACC
ACGGGGAGGTGTCGGACCTTCTCAAAGCAAAGAATCAAAATATGAGAGGGAATGGCAGAAAACAGTATCA
AGACTCACCTAATCAAAAGAAGAGAGCGAACGGAGTCCATGGTCAGCCGGCAAAGCAGCAGAATCCCTTG
GTGAAATGTGAAAAGAAGCTGCATCCACGGAAACTTCAGGACAACTTCGAGACAGATGCCACATGTGACC
TGCCTTGTTCTGGTGAAGAGCCGTCGGTAGAACATGCTGAGGGACATTCTGTGGCATGCAACGGACATTG
CAAGTTCCCATTTTCATCCAGAGCCTTTCTGAGTTTCAAGTTTGACCAAAATGCTATAATGAAAATCTTG
GACCTTTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_027490
Insert Size 1269 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_027490.1, NP_081766.1
RefSeq Size 8643 bp
RefSeq ORF 1269 bp
Locus ID 70640
UniProt ID Q9CYC6
Gene Summary Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs (PubMed:21070968). Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP (PubMed:21070968). Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay (By similarity). Plays a role in replication-dependent histone mRNA degradation (By similarity). Has higher activity towards mRNAs that lack a poly(A) tail (PubMed:21070968). Has no activity towards a cap structure lacking an RNA moiety (PubMed:21070968).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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