Tefm (NM_183275) Mouse Untagged Clone

CAT#: MC210743

Tefm (untagged) - Mouse RIKEN cDNA 1110002N22 gene (1110002N22Rik), (10ug)



  "NM_183275" in other vectors (4)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1110002N22Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC210743 representing NM_183275
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTGGAGGACAAACCTTGCTTGCCTCATCAAGGCCGGAGGGAGGAGATGGTTTCCAGTCCCAGAGT
ACTCATCCCTGCCTCCAGTCCTAAATAACACCTGCTCTGTGAGGAAATCCACTGCACCTGAGAAACGTGT
TGCCAGTGTTGCTGCTTGTGATACAGATGGAAAGGAGCCCGGGAATCCATTGGACAGGCTCTTCTCCCTG
GAGCAGCAGGCTTCTATCTTACAAGTGCTGAATACAGCTTCTGATAAAGAACTGGAAGCTTTCAGGTTGC
TTCGAGGAAGGAAGTCCGTCAATATTGTAGAACACAGAAAGAAGTTTGGGCCGTTTCAGAGGTTGGAGAA
TTTGATAGACGTGCCCTTGATCCAATATAAAACTGCTGTTGAAGTTTGTAACTCCATTCTCTGTCCGGAG
AACAGACGGAGGAAAAAGTCACAGGAGAAGTGGCTGCTGAGAAAGTTCATCAAGCCAGGTGTAGAGCAAG
AGAGACTTAAGGCAGTTAAGAGTATCGTATCTATTGTTTTTGGTACTCGAAGAATTGCATGGGCTCACCT
TGACCGGAGACCGACTGTGCTGGACTGGCAGCAGACTGAGTGCTGGAAATTAACTAACAAAACATACCCA
ACGTCCTTTTATCTGGAAGAGATTTCTTCAGTCATTTCAAAGATACCTAAAGCAGATCTCTACATTTTGG
AAAAATCAGGACTTTCCATTCAGAACACATCTCTGCTTCCTATACTGTTACACTTTCTTATCACGGAAGC
CATGCTGTATGCCTTACTCAACAAAACTTTTGCCGAGGATGGCCAGCACCGCGTGCTGAGCATAAATCGA
AATGCGGTGGGCAAGCACTTTGACCTGATGATCGGCGACACGAGGACGAGTGGGAGGGAGCTAGTGAAGC
AGTTCCTCTCTGAGTCTGTGCTGAAGGAGCGGCCTCGGGTGTTCTTCCCACAGGACCTCTTAGTGCAGTA
CAGACAGAAGGTTGTCAAGAGCTCATACCGGATTGAAGAGTTGTATGACTCATTATTACAAGCTGTTGCT
TTTTATGAGTTAGTTTTTGGCAAAGACTCAGAGCTCAAATGTTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_183275
Insert Size 1095 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_183275.2, NP_899098.2
RefSeq Size 1302 bp
RefSeq ORF 1095 bp
Locus ID 68550
UniProt ID Q5SSK3
Gene Summary Transcription elongation factor which increases mitochondrial RNA polymerase processivity. Regulates transcription of the mitochondrial genome, including genes important for the oxidative phosphorylation machinery (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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