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Rffl (NM_001164571) Mouse Untagged Clone
CAT＃: MC210529
Rffl (untagged) - Mouse ring finger and FYVE like domain containing protein (Rffl), transcript variant 5, (10ug)
CNY 5,856.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1700051E09Rik; 4930516L10Rik; BG080975; Carp2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001164571 |
| Insert Size | 1011 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001164571.1, NP_001158043.1 |
| RefSeq Size | 3425 bp |
| RefSeq ORF | 1011 bp |
| Locus ID | 67338 |
| Gene Summary | E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Mediates 'Lys-48'-linked polyubiquitination of PRR5L and its subsequent proteasomal degradation thereby indirectly regulating cell migration through the mTORC2 complex. Also ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate apoptosis downstream of death domain receptors. Also negatively regulates the tumor necrosis factor-mediated signaling pathway through targeting of RIPK1 to ubiquitin-mediated proteasomal degradation. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. May also play a role in endocytic recycling.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) uses an alternate in-frame splice site and lacks an alternate in-frame exon in the central coding region, compared to variant 1. The resulting isoform (5) is shorter than isoform 1. |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG217548 | Rffl (tGFP-tagged) - Mouse ring finger and FYVE like domain containing protein (Rffl) transcript variant 5, (10ug) |
CNY 3,710.00 |
|
| MR217548 | Rffl (Myc-DDK-tagged) - Mouse ring finger and FYVE like domain containing protein (Rffl), transcript variant 5 |
CNY 3,330.00 |
|
| MR217548L3 | Lenti ORF clone of Rffl (Myc-DDK-tagged) - Mouse ring finger and FYVE like domain containing protein (Rffl), transcript variant 5 |
CNY 5,230.00 |
|
| MR217548L4 | Lenti ORF clone of Rffl (mGFP-tagged) - Mouse ring finger and FYVE like domain containing protein (Rffl), transcript variant 5 |
CNY 5,230.00 |
