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Pnpla2 (NM_025802) Mouse Untagged Clone
CAT＃: MC210442
Pnpla2 (untagged) - Mouse patatin-like phospholipase domain containing 2 (Pnpla2), transcript variant 2, (10ug)
CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 0610039C21Rik; 1110001C14Rik; Atgl; TTS-2.2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC210442 representing NM_025802
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCCGAGGGAGACCAAGTGGAACATCTCATTCGCTGGCTGCGGCTTCCTCGGGGTCTACCACATTG GCGTGGCCTCCTGCCTCCGTGAGCACGCGCCCTTCCTGGTGGCCAACGCCACTCACATCTACGGAGCCTC GGCAGGGGCGCTCACCGCCACAGCGCTGGTCACTGGGGCCTGCCTGGGTGAAGCAGGTGCCAACATTATT GAGGTGTCCAAGGAGGCCCGGAAGCGGTTCCTGGGTCCTCTGCATCCCTCCTTCAACCTGGTGAAGACCA TCCGTGGCTGTCTACTAAAGACCCTGCCTGCTGATTGCCATGAGCGCGCCAATGGACGCCTGGGCATCTC CCTGACTCGTGTTTCAGACGGAGAGAACGTCATCATATCCCACTTTAGCTCCAAGGATGAGCTCATCCAG GCCAATGTCTGCAGCACATTTATCCCGGTGTACTGTGGCCTCATTCCTCCTACCCTCCAAGGGGTGCGCT ATGTGGATGGCGGCATTTCAGACAACTTGCCACTTTATGAGCTGAAGAATACCATCACAGTGTCCCCATT CTCAGGCGAGAGTGACATCTGCCCTCAGGACAGCTCCACCAACATCCACGAGCTTCGCGTCACCAACACC AGCATCCAGTTCAACCTTCGCAATCTCTACCGCCTCTCGAAGGCTCTCTTCCCGCCAGAGCCCATGGTCC TCCGAGAGATGTGCAAACAGGGCTACAGAGATGGACTTCGATTCCTTAGGAGGAATGCCCTGCTGGAGGC CTGTGTGGAACCAAAGGACCTGATGACCACCCTTTCCAACATGCTACCAGTGCGCCTGGCAACGGCCATG ATGGTGCCCTATACTCTGCCGCTGGAGAGTGCAGTGTCCTTCACCATCCGCTTGTTGGAGTGGCTGCCTG ATGTCCCTGAAGATATCCGGTGGATGAAAGAGCAGACGGGTAGCATCTGCCAGTATCTGGTGATGAGGGC CAAGAGGAAATTGGGTGACCATCTGCCTTCCAGACTGTCTGAGCAGGTGGAACTGCGACGTGCCCAGTCT CTGCCCTCTGTGCCACTGTCTTGCGCCACCTACAGTGAGGCCCTACCCAACTGGGTACGAAACAACCTCT CACTGGGGGACGCGCTGGCCAAGTGGGAAGAATGCCAGCGTCAGCTACTGCTGGGTCTCTTCTGCACCAA TGTGGCCTTCCCGCCGGATGCCTTGCGCATGCGCGCACCTGCCAGCCCCACTGCCGCAGATCCTGCCACC CCACAGGATCCACCTGGCCTCCCGCCTTGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_025802 |
| Insert Size | 1293 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_025802.3, NP_080078.2 |
| RefSeq Size | 2467 bp |
| RefSeq ORF | 1293 bp |
| Locus ID | 66853 |
| UniProt ID | Q8BJ56 |
| Gene Summary | Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets (PubMed:15550674). Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame coding exon, as compared to variant 1. The resulting isoform (2) lacks an internal segment, as compared to isoform 1. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Simplified assays of lipolysis enzymes for drug discovery and specificity assessment of known inhibitors
,null,
Journal of Lipid Research
,PubMed ID 26423520
[Pnpla2]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226261 | Pnpla2 (tGFP-tagged) - Mouse patatin-like phospholipase domain containing 2 (Pnpla2) transcript variant 2, (10ug) |
CNY 4,750.00 |
|
| MR226261 | Pnpla2 (Myc-DDK-tagged) - Mouse patatin-like phospholipase domain containing 2 (Pnpla2), transcript variant 2 |
CNY 5,488.00 |
|
| MR226261L3 | Lenti ORF clone of Pnpla2 (Myc-DDK-tagged) - Mouse patatin-like phospholipase domain containing 2 (Pnpla2), transcript variant 2 |
CNY 6,180.00 |
|
| MR226261L4 | Lenti ORF clone of Pnpla2 (mGFP-tagged) - Mouse patatin-like phospholipase domain containing 2 (Pnpla2), transcript variant 2 |
CNY 6,180.00 |
