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Asph (NM_133723) Mouse Untagged Clone
CAT＃: MC210256
Asph (untagged) - Mouse aspartate-beta-hydroxylase (Asph), transcript variant 2, (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2310005F16Rik; 3110001L23Rik; AI848629; AW261690; AW561901; BAH; C79816; cI-37 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC210256 representing NM_133723
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAAGATAAAGAGGCAAAGCACGGAGGACACAAGAATGGGAGAAGAGGAGGGATTTCAGGAGGGT CCTTTTTCACATGGTTCATGGTCATTGCATTGCTCGGCGTCTGGACATCTGTGGCTGTCGTGTGGTTTGA CTTGGTCGATTATGAAGAAGTTCTAGGAAAACTAGGAGTCTATGATGCGGATGGCGATGGAGACTTTGAT GTGGATGATGCCAAAGTTTTATTAGAAGGACCTGGTGGGTTAGCCAAGAGGAAAACTAAGGCTAAAGCTA AAGAACCCATCAAAGAAGAGCTGAAGAAGGAGAGAGGGAAAGCTGTGCCTAGCAAGAATGAAGAGCGAAG GCAGGGGAAGAAGGAGCAGGAGGATAGAGGGAAGGGGAGGAAGAAGCCTGACTCAGACACATCCCAGAAA GCATCCGCTGCAGGGAAGAGAGACAGAGACAAGGAGAAAGCCAGCTCTGACAAGAGTTCCAAGTCTAAGG AAAGCTGGAAGAAGGCCGTAGAGACAAAGGCTGTTTCTAGTAAAGTGGCTGCCAGAGACAAAGACAGGCG AGGAAGAAGCTCCTCTGGGCATGCTCACGTCGCCAAGGAAAATGGCCAGAAGAGAAAGAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_133723 |
| Insert Size | 624 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_133723.2, NP_598484.2 |
| RefSeq Size | 1761 bp |
| RefSeq ORF | 624 bp |
| Locus ID | 65973 |
| Gene Summary | Isoform 1: specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has multiple differences which result in distinct UTRs and coding end regions, compared to variant 1. The resulting protein (isoform 2) is shorter and has distinct N- and C-termini when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG224410 | Asph (tGFP-tagged) - Mouse aspartate-beta-hydroxylase (Asph) transcript variant 2, (10ug) |
CNY 2,280.00 |
|
| MR224410 | Asph (Myc-DDK-tagged) - Mouse aspartate-beta-hydroxylase (Asph), transcript variant 2 |
CNY 2,640.00 |
|
| MR224410L3 | Lenti ORF clone of Asph (Myc-DDK-tagged) - Mouse aspartate-beta-hydroxylase (Asph), transcript variant 2 |
CNY 3,990.00 |
|
| MR224410L4 | Lenti ORF clone of Asph (mGFP-tagged) - Mouse aspartate-beta-hydroxylase (Asph), transcript variant 2 |
CNY 3,990.00 |
