Asph (NM_133723) Mouse Untagged Clone

CAT#: MC210256

Asph (untagged) - Mouse aspartate-beta-hydroxylase (Asph), transcript variant 2, (10ug)



  "NM_133723" in other vectors (4)

CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2310005F16Rik; 3110001L23Rik; AI848629; AW261690; AW561901; BAH; C79816; cI-37
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC210256 representing NM_133723
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGAAGATAAAGAGGCAAAGCACGGAGGACACAAGAATGGGAGAAGAGGAGGGATTTCAGGAGGGT
CCTTTTTCACATGGTTCATGGTCATTGCATTGCTCGGCGTCTGGACATCTGTGGCTGTCGTGTGGTTTGA
CTTGGTCGATTATGAAGAAGTTCTAGGAAAACTAGGAGTCTATGATGCGGATGGCGATGGAGACTTTGAT
GTGGATGATGCCAAAGTTTTATTAGAAGGACCTGGTGGGTTAGCCAAGAGGAAAACTAAGGCTAAAGCTA
AAGAACCCATCAAAGAAGAGCTGAAGAAGGAGAGAGGGAAAGCTGTGCCTAGCAAGAATGAAGAGCGAAG
GCAGGGGAAGAAGGAGCAGGAGGATAGAGGGAAGGGGAGGAAGAAGCCTGACTCAGACACATCCCAGAAA
GCATCCGCTGCAGGGAAGAGAGACAGAGACAAGGAGAAAGCCAGCTCTGACAAGAGTTCCAAGTCTAAGG
AAAGCTGGAAGAAGGCCGTAGAGACAAAGGCTGTTTCTAGTAAAGTGGCTGCCAGAGACAAAGACAGGCG
AGGAAGAAGCTCCTCTGGGCATGCTCACGTCGCCAAGGAAAATGGCCAGAAGAGAAAGAACTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_133723
Insert Size 624 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_133723.2, NP_598484.2
RefSeq Size 1761 bp
RefSeq ORF 624 bp
Locus ID 65973
Gene Summary Isoform 1: specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) has multiple differences which result in distinct UTRs and coding end regions, compared to variant 1. The resulting protein (isoform 2) is shorter and has distinct N- and C-termini when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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