Cenpk (NM_021790) Mouse Untagged Clone

CAT#: MC210203

Cenpk (untagged) - Mouse centromere protein K (Cenpk), transcript variant 1, (10ug)



  "NM_021790" in other vectors (4)

CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms B130045K24Rik; BB232189; C530004N04Rik; Cenp-K; Solt; Solzt
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC210203 representing NM_021790
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTACCGTGTTCTGTCTATTCCAACAACAAAGCTGCATCCTGTACCACTTCTCCGTTGGTAATTTACA
GTAGTTGCAGTAAGGTGAAGAAAACTATAAAGGATATGTCAGAGAATAAACAAGAAGTACATCCAGATAC
TATTACAGATGTGGAAGCTGTTATAGATACAGAGGAAGAACTTATTAAAGAATGTGAAGAAATGTGGAAA
GACATGGAAGATTGTCAGAATAAATTATCACTTATTGGAACTGAGACACTCACGAATGCAGATGCTCAGC
TCTCATTACTTATTATGCAAATGAAATGTTTGACTGCTGAACTTGGTCAATGGAAGAAAAGAAAACCCGA
AATAATTCCCCTAAATGAAGACGTTTTGTTAACATTAGGAAAAGAAGAGTTCCAAAAATTGAGATGTGAT
CTTGAAATGGTACTATCTACCATTCAATCAAAGAATGAAAAGTTAAAAGAAGACTTAGAAAGGGAGCAAC
AGTGGTTGGATGAACAGCAGCAAATACTGGACACTCTTAACGTATTAAACAGTGATGTGGAAAATCAGGT
GGTAACATTAACTGAGTCAAGAATCTTTAATGAACTGACAACTAAGATCCGTGGGATAAAGGAATTCAAG
GAGAAGCTCTTGCTTACCTTGGGTGCTTTTTTAGACAATCATTTTCCTCTGCCTGAAGCAAGTACTCCAA
AGAAAAGGAAAAACATTCAAGACTCGAATGCACAGCTGATAACACTGAATGAAATATTAGAGATGCTTAT
AAATAGAATGTTTGATGTTCCACATGATCCATATGTTAAAATTCGTGATTCCTTTTGGCCACCATATATT
GAGCTACTTCTGCGTTACGGAATTGCTTTGAGACATCCAGAAGATCCATCCCAAATAAGGTTAGAAGCTT
TCCATCAGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_021790
Insert Size 921 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_021790.2, NP_068562.1
RefSeq Size 1222 bp
RefSeq ORF 921 bp
Locus ID 60411
UniProt ID Q9ESN5
Gene Summary Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with KNL1 to recruit the NDC80 complex to the outer kinetochore (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) uses alternate 5' exon structure, differs in the 5' UTR, and includes an alternate 3' terminal exon compared to variant 2. This transcript also initiates translation at an alternate start codon, resulting in isoform 1, which is longer and has distinct N- and C-termini compared to isoform 2.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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