H1f5 (NM_020034) Mouse Untagged Clone

CAT#: MC210088

Hist1h1b (untagged) - Mouse histone cluster 1, H1b (Hist1h1b), (10ug)



  "NM_020034" in other vectors (4)

CNY 3,990.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms H1-5; H1.5; H1B; H1s-; H1s-3; Hist1h; Hist1h1b
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC210088 representing NM_020034
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGAGACTGCTCCCGCTGAGACCGCGGCTCCGGCTCCTGTAGAGAAGTCTCCCGCCAAGAAGAAGA
CAACGAAAAAAGCTGGCGCGGCGAAGCGCAAGGCCACTGGCCCCCCGGTGTCCGAGCTCATCACTAAGGC
TGTTTCTGCCTCTAAGGAGCGCGGCGGCGTGTCCCTGCCTGCCCTTAAGAAGGCACTGGCTGCTGGTGGC
TACGATGTGGAGAAGAACAACAGCCGCATCAAGCTTGGGCTCAAGAGTCTGGTGAGCAAGGGTACCCTGG
TGCAGACCAAGGGCACCGGCGCCTCTGGTTCCTTCAAGCTTAACAAGAAGGCGGCTTCTGGCGAGGCCAA
GCCCAAGGCTAAGAAGACCGGGGCTGCCAAGGCCAAGAAGCCTGCAGGTGCTACCCCTAAAAAGCCTAAG
AAGACTGCGGGGGCAAAGAAGACCGTGAAGAAAACTCCAAAGAAAGCGAAGAAGCCTGCGGCGGCTGGAG
TGAAGAAAGTTGCCAAGAGTCCTAAGAAGGCCAAGGCTGCTGCCAAGCCTAAAAAAGCAGCAAAGAGCCC
GGCCAAGCCCAAGGCGGTGAAGTCTAAGGCATCCAAACCTAAGGTTACCAAGCCTAAGACCGCTAAGCCT
AAGGCTGCCAAGGCCAAGAAGGCTGTTTCCAAAAAGAAGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_020034
Insert Size 672 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_020034.2, NP_064418.1
RefSeq Size 794 bp
RefSeq ORF 672 bp
Locus ID 56702
UniProt ID P43276
Gene Summary Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. [provided by RefSeq, Sep 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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