Wnt5b (NM_009525) Mouse Untagged Clone

CAT#: MC209654

Wnt5b (untagged) - Mouse wingless-related MMTV integration site 5B (Wnt5b), (10ug)



  "NM_009525" in other vectors (4)

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AW545702; Wnt-5b
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC209654 representing NM_009525
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGGTCCCAGGGCATTGGGATGGGTTGAGGCCGGCCATGCCCAGCCTGCTGCTGGTGGTCGTGGCAG
CTCTGCTCTCCAGCTGGGCACAGCTGCTGACTGACGCCAACTCCTGGTGGTCACTAGCTCTGAACCCAGT
GCAGAGACCGGAGATGTTCATCATTGGCGCTCAGCCCGTGTGCAGCCAACTTCCTGGGCTTTCCCCAGGC
CAGAGAAAGCTGTGTCAGTTGTATCAGGAGCACATGTCCTACATCGGGGAGGGAGCCAAGACGGGCATCA
GAGAGTGCCAACACCAGTTTCGACAGAGGCGCTGGAACTGCAGCACCGTGGACAACACATCTGTCTTTGG
CAGAGTTATGCAGATAGGTAGCCGAGAGACTGCCTTCACGTATGCAGTGAGCGCCGCTGGCGTGGTGAAT
GCCATCAGCCGAGCCTGCAGAGAGGGTGAGCTGTCCACCTGTGGCTGCAGCCGTGCTGCGAGGCCCAAGG
ACCTGCCTCGGGACTGGCTGTGGGGTGGCTGTGGAGACAACGTGGAGTACGGCTACCGCTTTGCCAAGGA
GTTTGTGGATGCCCGAGAGCGTGAGAAGAACTTTGCCAAGGGATCGGAGGAGCAGGGCCGAGCTCTCATG
AACCTACAGAACAACGAGGCTGGCCGCCGGGCCGTGTATAAGATGGCTGATGTCGCCTGCAAATGTCACG
GAGTCTCCGGGTCCTGCAGCCTCAAGACCTGCTGGCTCCAGCTGGCCGAGTTCCGCAAGGTTGGGGACCG
TTTGAAGGAGAAGTACGACAGCGCCGCGGCCATGCGCATCACCCGCCAGGGCAAGCTGGAGCTGGCCAAC
AGCCGCTTCAACCAGCCCACCCCAGAGGACCTGGTCTACGTGGACCCCAGTCCTGACTACTGCTTGCGTA
ATGAGACCACAGGCTCCCTGGGCACCCAGGGTCGCCTCTGCAACAAGACCTCAGAGGGCATGGACGGCTG
CGAGCTCATGTGCTGTGGCCGCGGCTATGACCGCTTCAAGAGCGTTCAGGTGGAACGCTGCCACTGCAGG
TTCCACTGGTGTTGCTTTGTCAGATGCAAAAAATGCACCGAGGTTGTGGACCAGTATGTCTGTAAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_009525
Insert Size 1119 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC010775, AAH10775
RefSeq Size 2384 bp
RefSeq ORF 1080 bp
Locus ID 22419
UniProt ID P22726
Gene Summary Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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