Wnt10b (NM_011718) Mouse Untagged Clone

CAT#: MC209647

Wnt10b (untagged) - Mouse wingless related MMTV integration site 10b (Wnt10b), (10ug)



  "NM_011718" in other vectors (4)

CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Wnt12
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC209647 representing NM_011718
Red=Cloning site Blue=ORF

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGGAGGAGCCCCGGTCTCGGCCTCCGCCCTTAGGCCTCGCGGGTCTCCTGTTCTTGGCTTTGTTCA
GTCGGGCTCTAAGCAATGAGATTCTGGGCCTTAAACTTCCCGGTGAGCCGCCGCTGACGGCCAACACCGT
GTGCTTGACCCTGTCCGGACTGAGTAAGCGACAGCTGGGGCTGTGCCTGCGCAGCCCCGACGTGACGGCG
TCGGCGCTCCAGGGGCTGCACATCGCCGTTCACGAGTGTCAGCACCAGCTGCGCGACCAGCGCTGGAACT
GCTCGGCACTGGAGGGCGGCGGCCGGCTGCCGCACCACAGCGCCATCCTCAAGCGCGGTTTCCGTGAGAG
TGCTTTCTCCTTCTCCATGCTGGCTGCTGGGGTCATGCATGCTGTTGCCACAGCCTGCAGCCTGGGCAAG
CTGGTGAGCTGCGGCTGCGGATGGAAGGGTAGTGGTGAGCAAGACCGGCTTAGAGCCAAGCTGCTGCAGC
TTCAGGCACTGTCTCGGGGCAAGACTTTCCCCATCTCCCAGCCCAGCCCTGTTCCTGGCTCAGTCCCCAG
CCCCGGCCCCCAGGACACGTGGGAATGGGGTGGCTGTAACCACGACATGGACTTCGGAGAGAAGTTCTCT
CGGGATTTCTTGGATTCCAGGGAGGCTCCCCGGGACATCCAGGCGAGAATGCGGATCCACAACAACAGGG
TGGGACGCCAGGTGGTAACGGAAAACCTGAAGCGGAAGTGCAAATGCCATGGAACGTCAGGCAGCTGCCA
ATTCAAGACCTGTTGGAGGGCAGCGCCAGAGTTCCGGGCCATCGGGGCAGCACTGAGGGAGCGGCTGAGC
AGAGCCATCTTTATCGATACCCACAACCGCAACTCTGGAGCGTTCCAGCCCCGCCTACGTCCGCGGCGCC
TCTCTGGAGAGCTGGTTTACTTTGAGAAGTCTCCTGACTTCTGCGAGCGAGACCCTACTCTGGGCTCCCC
AGGCACGAGAGGCCGGGCTTGCAACAAGACCAGCCGCCTCTTGGATGGCTGTGGCAGCCTGTGCTGTGGC
CGTGGGCACAACGTGCTCCGGCAGACGCGAGTGGAGCGCTGCCACTGTCGTTTCCACTGGTGCTGTTATG
TGCTGTGTGATGAGTGTAAAGTCACAGAGTGGGTCAATGTGTGTAAATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_011718
Insert Size 1170 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_011718.2, NP_035848.1
RefSeq Size 2272 bp
RefSeq ORF 1170 bp
Locus ID 22410
UniProt ID P48614
Gene Summary Member of the Wnt ligand gene family that encodes for secreted proteins, which activate the Wnt signaling cascade. Specifically activates canonical Wnt/beta-catenin signaling and thus triggers beta-catenin/LEF/TCF-mediated transcriptional programs. Involved in signaling networks controlling stemness, pluripotency and cell fate decisions. Acts in the immune system, mammary gland, adipose tissue, bone and skin.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...