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Tnnt2 (NM_001130175) Mouse Untagged Clone
CAT＃: MC209562
Tnnt2 (untagged) - Mouse troponin T2, cardiac (Tnnt2), transcript variant 2, (10ug)
CNY 3,600.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | cTnT; Tnt |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC209562 representing NM_001130175
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTGCCCTCAGCCAGTCCCTGCTGAGGCTGAACAGATACCTCAAGACCTGTGTGCAGTCCCTGTTC AGAGAGCCGAGAGCATGTCTGACGCCGAGGAGGTGGTGGAGGAGTACGAGGAGGAACAGGAAGAAGCTGT GGAAGAGGAAGACTGGAGTGAAGAAGAGGAGGACGAGCAAGAGGAGGCAGTGGAGGAGGAGGAGGCTGGT GGGGCTGAACCTGAGCCTGAGGGTGAGGCTGAGACAGAGGAGGCCAACGTAGAAGAGGTTGGTCCTGATG AAGAAGCCAAAGATGCTGAAGAAGGTCCAGTAGAGGACACCAAACCCAAGCCCAGCAGGCTCTTCATGCC CAACTTGGTGCCACCCAAGATCCCCGATGGAGAGAGAGTGGACTTTGATGACATCCACAGGAAGCGCGTG GAGAAGGACCTGAATGAGCTACAGACTCTGATCGAGGCTCACTTCGAGAACAGGAAGAAGGAGGAAGAGG AGCTGATTTCCCTCAAAGACAGGATCGAAAAGCGTCGGGCAGAGCGGGCCGAGCAGCAGCGTATTCGCAA TGAGCGGGAGAAGGAAAGGCAGAACCGCCTGGCTGAAGAGAGGGCCCGGCGTGAGGAGGAGGAGAACAGG AGGAAGGCTGAGGATGAGGCCCGGAAGAAGAAGGCTCTGTCCAACATGATGCACTTTGGAGGGTACATCC AGAAGACAGAGCGGAAGAGTGGGAAGAGACAGACAGAGAGAGAGAAGAAGAAGAAGATCCTGGCAGAGAG GAGGAAGGCGCTGGCCATCGACCACCTGAATGAAGACCAACTGAGAGAGAAGGCCAAGGAGCTGTGGCAG AGTATTCACAACCTGGAGGCTGAGAAGTTCGACCTGCAGGAAAAGTTCAAGCAGCAGAAATACGAAATCA ACGTTCTGCGAAACCGGATCAATGACAACCAGAAAGTCTCCAAAACTCGTGGGAAGGCCAAAGTCACCGG GCGTTGGAAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001130175 |
| Insert Size | 993 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001130175.1, NP_001123647.1 |
| RefSeq Size | 1228 bp |
| RefSeq ORF | 993 bp |
| Locus ID | 21956 |
| Gene Summary | Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate splice site in the 5' region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (g) has a distinct N-terminus and is longer than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG224382 | Tnnt2 (tGFP-tagged) - Mouse troponin T2 cardiac (Tnnt2) transcript variant 2, (10ug) |
CNY 4,370.00 |
|
| MR224382 | Tnnt2 (Myc-DDK-tagged) - Mouse troponin T2, cardiac (Tnnt2), transcript variant 2 |
CNY 3,840.00 |
|
| MR224382L1 | Lenti ORF clone of Tnnt2 (Myc-DDK-tagged) - Mouse troponin T2, cardiac (Tnnt2), transcript variant 2 |
CNY 5,890.00 |
|
| MR224382L2 | Lenti ORF clone of Tnnt2 (mGFP-tagged) - Mouse troponin T2, cardiac (Tnnt2), transcript variant 2 |
CNY 6,240.00 |
|
| MR224382L3 | Lenti ORF clone of Tnnt2 (Myc-DDK-tagged) - Mouse troponin T2, cardiac (Tnnt2), transcript variant 2 |
CNY 5,890.00 |
|
| MR224382L4 | Lenti ORF clone of Tnnt2 (mGFP-tagged) - Mouse troponin T2, cardiac (Tnnt2), transcript variant 2 |
CNY 5,890.00 |
