United States
Germany
Japan
United Kingdom
China
Tead1 (NM_009346) Mouse Untagged Clone
CAT＃: MC209509
Tead1 (untagged) - Mouse TEA domain family member 1 (Tead1), transcript variant 2, (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2610024B07Rik; B230114H05Rik; Gtrgeo5; mTEF-1; Tcf13; TEAD-1; TEF-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC209509 representing NM_009346
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAGGATGAGCGACTCGGCAGATAAGCCGATTGACAACGACGCGGAGGGCGTCTGGAGTCCTGATA TTGAGCAGAGTTTCCAGGAGGCCCTGGCTATCTATCCGCCGTGTGGGAGGAGAAAAATCATCTTATCAGA CGAAGGCAAAATGTATGGTAGAAATGAATTGATAGCCAGATACATCAAACTCAGGACGGGAAAGACAAGG ACCAGGAAGCAGGTGTCTAGTCACATTCAGGTTCTTGCCAGAAGGAAATCTCGTGATTTTCATTCCAAGC TGAAGGTAACAAGCATGGATCAGACTGCCAAGGACAAGGCCCTGCAGCACATGGCTGCCATGTCATCAGC CCAGATCGTCTCGGCTACTGCCATCCACAACAAGCTGGGGCTGCCTGGGATTCCACGCCCCACCTTCCCG GGGGGTCCGGGGTTCTGGCCTGGGATGATACAGACAGGACAGCCAGGATCCTCACAAGACGTCAAGCCCT TTGTGCAGCAGGCCTACCCCATCCAGCCAGCAGTCACAGCCCCCATTCCAGGGTTTGAGCCTACGTCAGC CCCAGCCCCCTCAGTTCCTGCCTGGCAGGGCCGATCCATTGGCACAACCAAGCTTCGCCTGGTGGAATTC TCCGCTTTCCTTGAACAGCAGAGAGACCCAGACTCGTACAACAAACACCTCTTCGTGCACATCGGGCATG CCAACCATTCTTACAGTGACCCGTTGCTCGAATCTGTGGACATTCGTCAGATATATGACAAATTTCCTGA AAAGAAAGGTGGCTTGAAGGAGCTGTTTGGAAAGGGCCCTCAAAACGCCTTCTTCCTCGTCAAATTCTGG GCGGACTTAAACTGCAATATCCAAGACGACGCCGGGGCCTTTTATGGTGTGAGCAGTCAGTATGAGAGTT CTGAGAACATGACAGTTACCTGTTCCACCAAAGTGTGCTCCTTTGGGAAACAAGTAGTAGAAAAAGTAGA GACGGAGTATGCGAGGTTCGAGAATGGTCGATTCGTGTACCGAATAAACCGCTCGCCAATGTGTGAATAT ATGATCAACTTCATCCACAAGCTCAAACACCTACCAGAGAAATATATGATGAACAGTGTTTTGGAAAACT TCACCATATTATTGGTGGTAACAAACAGGGATACACAAGAAACTCTGCTCTGCATGGCCTGTGTATTTGA AGTCTCGAATAGCGAACACGGAGCACAGCACCATATCTACAGGCTTGTGAAGGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_009346 |
| Insert Size | 1248 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_009346.3, NP_033372.1 |
| RefSeq Size | 9899 bp |
| RefSeq ORF | 1248 bp |
| Locus ID | 21676 |
| Gene Summary | Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1 and uses a non-AUG (AUU) start codon for translation initiation. The resulting isoform (2) lacks an internal protein segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG221632 | Tead1 (tGFP-tagged) - Mouse TEA domain family member 1 (Tead1) transcript variant 2, (10ug) |
CNY 4,560.00 |
|
| MR221632 | Tead1 (Myc-DDK-tagged) - Mouse TEA domain family member 1 (Tead1), transcript variant 2 |
CNY 4,180.00 |
|
| MR221632L3 | Lenti ORF clone of Tead1 (Myc-DDK-tagged) - Mouse TEA domain family member 1 (Tead1), transcript variant 2 |
CNY 6,080.00 |
|
| MR221632L4 | Lenti ORF clone of Tead1 (mGFP-tagged) - Mouse TEA domain family member 1 (Tead1), transcript variant 2 |
CNY 6,080.00 |
