Sphk1 (NM_011451) Mouse Untagged Clone

CAT#: MC209438

Sphk1 (untagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 1, (10ug)



  "NM_011451" in other vectors (6)

CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1110006G24Rik; Sk1; Spk1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC209438 representing NM_011451
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGTGGTGTTGTGTTTTGTTTGTAGTAGAATGCCCTCGAGGACTGCTCCCACGGCCATGCAGAGTGC
TGGTGCTGCTGAACCCCCAGGGTGGCAAGGGCAAGGCTCTGCAGCTCTTCCAGAGCCGTGTGCAGCCCTT
CCTGGAGGAGGCAGAGATAACCTTTAAACTGATACTCACCGAACGGAAGAACCATGCCAGGGAGCTGGTG
TGTGCAGAGGAGTTGGGTCACTGGGACGCCCTGGCAGTCATGTCCGGTGATGGTCTGATGCATGAGGTGG
TGAATGGGCTAATGGAACGGCCAGACTGGGAGACTGCCATCCAGAAACCCCTGTGTAGCCTCCCTGGAGG
CTCCGGCAATGCGCTGGCAGCTTCTGTGAACCACTATGCTGGGTACGAGCAGGTGACTAATGAAGACCTG
CTCATCAACTGCACACTGCTGTTGTGCCGCCGGCGCCTGTCACCCATGAACCTGCTGTCCCTGCACACTG
CTTCTGGGCTGCGGCTCTATTCTGTGCTCAGTCTGTCCTGGGGCTTTGTTGCTGACGTGGACCTCGAGAG
TGAGAAGTACAGGCGCTTGGGGGAGATTCGTTTCACAGTGGGCACCTTCTTTCGCCTAGCAAGCCTGCGC
ATCTACCAAGGCCAACTGGCCTACCTTCCTGTAGGAACTGTGGCCTCTAAGAGACCCGCCTCTACACTGG
TGCAGAAGGGCCCCGTCGACACACACCTTGTTCCTCTGGAGGAGCCAGTGCCTTCTCATTGGACTGTGGT
ACCAGAACAGGACTTCGTCCTGGTGCTGGTGCTGCTACACACCCACCTGAGCTCCGAGCTGTTTGCAGCA
CCCATGGGCCGCTGTGAGGCTGGTGTTATGCATCTGTTCTACGTACGTGCGGGGGTGTCAAGGGCTGCGC
TGCTGCGCCTCTTCCTGGCCATGCAGAAGGGCAAGCATATGGAACTTGACTGTCCATACCTGGTTCATGT
GCCCGTGGTTGCTTTCCGCCTGGAGCCCAGGAGCCAGAGGGGCGTGTTTTCTGTGGATGGAGAGCTGATG
GTATGTGAAGCTGTGCAGGGCCAAGTGCACCCAAACTACCTTTGGATGGTCTGTGGCAGCAGAGATGCCC
CATCCGGCCGGGACTCCCGGCGGGGGCCACCTCCAGAAGAACCATAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_011451
Insert Size 1167 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_011451.3, NP_035581.1
RefSeq Size 1537 bp
RefSeq ORF 1167 bp
Locus ID 20698
UniProt ID Q8CI15
Gene Summary This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
Transcript Variant: This variant (1) represents the shortest transcript and encodes the longest isoform (1). This isoform is also known as Sphk1b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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