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Sphk1 (NM_025367) Mouse Untagged Clone
CAT＃: MC209435
Sphk1 (untagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 2, (10ug)
CNY 3,656.00
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1110006G24Rik; SK; Sk1; Spk1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC209435 representing NM_025367.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAACCAGTAGAATGCCCTCGAGGACTGCTCCCACGGCCATGCAGAGTGCTGGTGCTGCTGAACCCC CAGGGTGGCAAGGGCAAGGCTCTGCAGCTCTTCCAGAGCCGTGTGCAGCCCTTCCTGGAGGAGGCAGAG ATAACCTTTAAACTGATACTCACCGAACGGAAGAACCATGCCAGGGAGCTGGTGTGTGCAGAGGAGTTG GGTCACTGGGACGCCCTGGCAGTCATGTCCGGTGATGGTCTGATGCATGAGGTGGTGAATGGGCTAATG GAACGGCCCGACTGGGAGACTGCCATCCAGAAACCCCTGTGTAGCCTCCCTGGAGGCTCCGGCAATGCG CTGGCAGCTTCTGTGAACCACTATGCTGGGTACGAACAGGTGACTAATGAAGACCTGCTCATCAACTGC ACACTGCTGTTGTGCCGCCGGCGCCTGTCACCCATGAACCTGCTGTCCCTGCACACTGCTTCTGGGCTG CGGCTCTATTCTGTGCTCAGTCTGTCCTGGGGCTTTGTTGCTGACGTGGACCTCGAGAGTGAGAAGTAC AGGCGCTTGGGGGAGATTCGTTTCACAGTGGGCACCTTCTTTCGCCTAGCAAGCCTGCGCATCTACCAA GGCCAACTGGCCTACCTTCCTGTAGGAACTGTGGCCTCTAAGAGACCCGCCTCTACACTGGTGCAGAAG GGCCCCGTCGACACACACCTTGTTCCTCTGGAGGAGCCAGTGCCTTCTCATTGGACTGTGGTACCAGAA CAGGACTTTGTCCTGGTGCTGGTGCTGCTACACACCCACCTGAGCTCCGAGCTGTTTGCAGCACCCATG GGCCGCTGTGAGGCTGGTGTTATGCATCTGTTCTACGTACGTGCGGGGGTGTCAAGGGCTGCGCTGCTG CGCCTCTTCCTGGCCATGCAGAAGGGCAAGCATATGGAACTTGACTGTCCATACCTGGTTCATGTGCCC GTGGTTGCTTTCCGCCTGGAGCCCAGGAGCCAGAGGGGCGTGTTTTCTGTGGATGGAGAGCTGATGGTA TGTGAAGCTGTGCAGGGCCAAGTGCACCCAAACTACCTTTGGATGGTCTGTGGCAGCAGAGATGCCCCA TCCGGCCGGGACTCCCGGCGGGGGCCACCTCCAGAAGAACCATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites |
SgfI-MluI
Plasmid Map
|
| ACCN | NM_025367 |
| Insert Size | 1149 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC037710 |
| RefSeq Size | 1687 bp |
| RefSeq ORF | 1149 bp |
| Locus ID | 20698 |
| UniProt ID | Q8CI15 |
| MW | 42.4 kDa |
| Gene Summary | This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010] Transcript Variant: This variant (2) differs in the 5' UTR, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Variants 2, 3 and 4 encode the same protein (isoform 2). This isoform is also known as Sphk1a2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226229 | Sphk1 (tGFP-tagged) - Mouse sphingosine kinase 1 (Sphk1) transcript variant 2, (10ug) |
CNY 5,624.00 |
|
| MR226229 | Sphk1 (Myc-DDK-tagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 2 |
CNY 3,800.00 |
|
| MR226229L3 | Lenti ORF clone of Sphk1 (Myc-DDK-tagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 2 |
CNY 5,700.00 |
|
| MR226229L4 | Lenti ORF clone of Sphk1 (mGFP-tagged) - Mouse sphingosine kinase 1 (Sphk1), transcript variant 2 |
CNY 5,700.00 |
