Rbbp4 (NM_009030) Mouse Untagged Clone

CAT#: MC209297

Rbbp4 (untagged) - Mouse retinoblastoma binding protein 4 (Rbbp4), (10ug)



  "NM_009030" in other vectors (4)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms mRbAp48; RBAP48
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC209297 representing NM_009030
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGACAAGGAAGCGGCCTTTGACGACGCAGTGGAAGAACGGGTGATCAACGAGGAGTACAAAATAT
GGAAGAAGAACACCCCTTTTCTTTATGATTTGGTGATGACCCATGCCCTGGAGTGGCCCAGCTTAACTGC
CCAGTGGCTTCCAGATGTGACCAGGCCTGAAGGGAAAGATTTCAGCATTCATCGGCTTGTCCTGGGAACA
CACACATCTGATGAACAGAACCACCTGGTGATTGCCAGTGTCCAGCTCCCTAATGATGATGCTCAGTTTG
ATGCATCCCATTATGACAGTGAAAAAGGAGAATTTGGAGGTTTTGGTTCTGTCAGTGGGAAAATTGAAAT
AGAAATCAAGATCAACCATGAAGGAGAAGTGAACAGGGCCCGGTACATGCCTCAGAACCCCTGCATCATT
GCAACAAAGACTCCATCCAGTGATGTTCTTGTTTTTGACTACACAAAGCACCCTTCTAAACCAGACCCTT
CTGGAGAATGCAACCCCGATTTGCGTCTCCGTGGACATCAGAAGGAAGGTTATGGGCTTTCTTGGAATCC
AAATCTCAGTGGGCACTTACTTAGTGCTTCAGATGACCATACCATCTGCCTGTGGGACATCAGTGCAGTT
CCAAAAGAAGGAAAGGTGGTGGATGCAAAGACCATCTTCACGGGGCATACAGCAGTAGTGGAGGACGTGT
CCTGGCATCTGCTCCACGAGTCTCTATTTGGCTCAGTTGCTGATGACCAGAAGCTTATGATTTGGGACAC
TCGTTCAAACAATACTTCCAAGCCAAGCCACTCGGTTGATGCTCACACAGCTGAAGTGAATTGCTTATCT
TTCAATCCTTATAGTGAGTTCATTCTTGCCACAGGATCGGCTGACAAGACTGTTGCCTTGTGGGATCTGA
GGAATCTGAAACTCAAGTTGCACTCCTTTGAATCCCATAAGGATGAAATATTCCAAGTTCAGTGGTCACC
TCACAATGAGACTATCTTAGCTTCCAGTGGTACCGATCGTAGGCTGAATGTCTGGGATCTAAGTAAAATT
GGAGAAGAGCAGTCCCCAGAAGATGCAGAGGATGGCCCACCAGAATTGTTGTTTATTCATGGTGGTCACA
CTGCCAAGATATCTGACTTCTCCTGGAATCCCAATGAGCCTTGGGTGATTTGTTCTGTATCAGAAGACAA
TATCATGCAAGTGTGGCAGATGGCGGAGAACATTTACAATGATGAAGATCCTGAAGGAAGTGTGGATCCA
GAGGGACAAGGGTCCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_009030
Insert Size 1278 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_009030.3, NP_033056.2
RefSeq Size 4407 bp
RefSeq ORF 1278 bp
Locus ID 19646
UniProt ID Q60972
Gene Summary Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; and the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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