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Sirpa (NM_001177647) Mouse Untagged Clone
CAT＃: MC209262
Sirpa (untagged) - Mouse signal-regulatory protein alpha (Sirpa), transcript variant 3, (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI835480; Bit; CD172a; P84; Ptpns1; SHP-1; SHPS-1; SIRP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC209262 representing NM_001177647
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCGCCGGCCCGGCCCCTGGCCGCCTAGGGCCGCTGCTGCTCTGCCTGCTGCTCTCCGCGTCCT GTTTCTGTACAGGAGCCACGGGGAAGGAACTGAAGGTGACTCAGCCTGAGAAATCAGTGTCTGTTGCTGC TGGGGATTCGACCGTTCTGAACTGCACTTTGACCTCCTTGTTGCCGGTGGGACCCATTAGGTGGTACAGA GGAGTAGGGCCAAGCCGGCTGTTGATCTACAGTTTCGCAGGAGAATACGTTCCTCGAATTAGAAATGTTT CAGATACTACTAAGAGAAACAATATGGACTTTTCCATCCGTATCAGTAATGTCACCCCAGCAGATGCTGG CATCTACTACTGTGTGAAGTTCCAGAAAGGATCATCAGAGCCTGACACAGAAATACAATCTGGAGGGGGA ACAGAGGTCTATGTACTCGATAATAATGCTACCCACAACTGGAATGTCTTCATCGGTGTGGGCGTGGCGT GTGCTTTGCTCGTAGTCCTGCTGATGGCTGCTCTCTACCTCCTCCGGATCAAACAGAAGAAAGCCAAGGG GTCAACATCTTCCACACGGTTGCACGAGCCCGAGAAGAACGCCAGGGAAATAACCCAGATCCAGGACACA AATGACATCAACGACATCACATACGCAGACCTGAATCTGCCCAAAGAGAAGAAGCCCGCACCCCGGGCCC CTGAGCCTAACAACCACACAGAATATGCAAGCATTGAGACAGGCAAAGTGCCTAGGCCAGAGGATACCCT CACCTATGCTGACCTGGACATGGTCCACCTCAGCCGGGCACAGCCAGCCCCCAAGCCTGAGCCATCTTTC TCAGAGTATGCTAGTGTCCAGGTCCAGAGGAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001177647 |
| Insert Size | 876 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001177647.2, NP_001171118.1 |
| RefSeq Size | 3377 bp |
| RefSeq ORF | 876 bp |
| Locus ID | 19261 |
| UniProt ID | P97797 |
| Gene Summary | Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks two alternate in-frame exons in the central coding region, and uses an alternate in-frame splice site in the 3' coding region, compared to variant 4. The encoded isoform (2) is shorter than isoform 3. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227660 | Sirpa (tGFP-tagged) - Mouse signal-regulatory protein alpha (Sirpa) transcript variant 3, (10ug) |
CNY 4,240.00 |
|
| MR227660 | Sirpa (Myc-DDK-tagged) - Mouse signal-regulatory protein alpha (Sirpa), transcript variant 3 |
CNY 2,950.00 |
|
| MR227660L3 | Lenti ORF clone of Sirpa (Myc-DDK-tagged) - Mouse signal-regulatory protein alpha (Sirpa), transcript variant 3 |
CNY 4,850.00 |
|
| MR227660L4 | Lenti ORF clone of Sirpa (mGFP-tagged) - Mouse signal-regulatory protein alpha (Sirpa), transcript variant 3 |
CNY 4,850.00 |
