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Polb (NM_011130) Mouse Untagged Clone
CAT＃: MC209200
Polb (untagged) - Mouse polymerase (DNA directed), beta (Polb), (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | A430088C08Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC209200 representing NM_011130
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAAACGCAAGGCGCCGCAGGAGACCCTCAACGGCGGCATCACGGACATGCTCGTGGAACTCGCAA ACTTTGAGAAGAACGTGAGCCAGGCGATCCACAAGTACAATGCGTACAGAAAAGCGGCATCTGTGATAGC CAAGTACCCACACAAAATCAAGAGCGGAGCGGAAGCTAAGAAACTGCCAGGAGTAGGAACAAAAATTGCT GAAAAGATTGATGAATTTTTAGCAACTGGAAAATTGCGTAAACTGGAAAAGATTCGTCAAGATGATACAA GTTCATCCATCAATTTCCTGACTCGAGTTACTGGCATTGGACCATCTGCTGCAAGGAAGTTTGTAGATGA AGGAATTAAGACATTAGAAGATCTCAGGAAAAATGAAGATAAACTGAACCATCATCAACGAATTGGGCTG AAATATTTCGAGGACTTTGAAAAGAGAATTCCTCGTGAGGAAATGCTGCAGATGCAGGATATTGTACTTA ATGAAATTAAAAAAGTGGACTCTGAGTACATTGCTACAGTCTGTGGCAGTTTCAGAAGAGGCGCAGAGTC GAGTGGAGACATGGATGTCCTGCTGACCCACCCAAACTTCACGTCAGAATCCAGCAAACAGCCAAAGTTG TTACATCGTGTTGTGGAACAGTTACAAAAAGTCCATTTCATTACAGATACTCTGTCAAAGGGTGAAACAA AGTTCATGGGTGTTTGCCAGCTTCCCAGCGAGAAGGATGGAAAGGAATATCCACACAGGAGAATCGATAT CAGGTTGATCCCCAAAGATCAGTACTACTGTGGTGTTCTCTACTTCACTGGGAGTGACATCTTTAATAAG AACATGAGAGCGCACGCCCTGGAAAAGGGCTTCACAATCAATGAGTACACCATCCGCCCCCTGGGGGTCA CTGGAGTCGCTGGGGAGCCCCTTCCTGTGGACAGTGAGCAGGACATTTTTGATTACATCCAGTGGCGCTA CCGGGAGCCCAAGGATAGAAGTGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_011130 |
| Insert Size | 1008 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_011130.2, NP_035260.1 |
| RefSeq Size | 1227 bp |
| RefSeq ORF | 1008 bp |
| Locus ID | 18970 |
| UniProt ID | Q8K409 |
| Gene Summary | Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG224695 | Polb (tGFP-tagged) - Mouse polymerase (DNA directed) beta (Polb), (10ug) |
CNY 3,140.00 |
|
| MR224695 | Polb (Myc-DDK-tagged) - Mouse polymerase (DNA directed), beta (Polb) |
CNY 3,656.00 |
|
| MR224695L3 | Lenti ORF clone of Polb (Myc-DDK-tagged) - Mouse polymerase (DNA directed), beta (Polb) |
CNY 4,750.00 |
|
| MR224695L4 | Lenti ORF clone of Polb (mGFP-tagged) - Mouse polymerase (DNA directed), beta (Polb) |
CNY 4,750.00 |
