Smad7 (NM_001042660) Mouse Untagged Clone

CAT#: MC208925

Smad7 (untagged) - Mouse MAD homolog 7 (Drosophila) (Smad7), (10ug)



  "NM_001042660" in other vectors (6)

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Madh7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC208925 representing NM_001042660
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCAGGACCAAACGATCTGCGCTCGTCCGGCGTCTCTGGAGGAGCCGTGCGCCCGGCGGCGAGGACG
AGGAGGAGGGCGTGGGGGGTGGCGGCGGAGGAGGCGAGCTGCGGGGAGAAGGGGCGACGGACGGCCGGGC
TTATGGGGCTGGTGGCGGCGGTGCGGGCAGGGCTGGCTGCTGCCTGGGCAAGGCAGTCCGAGGTGCCAAA
GGTCACCACCATCCCCATCCCCCAACCTCGGGTGCCGGGGCGGCCGGGGGCGCCGAGGCGGATCTGAAGG
CGCTCACGCACTCGGTGCTCAAGAAACTCAAGGAGCGGCAGCTGGAGCTGCTGCTTCAGGCCGTGGAGTC
CCGCGGCGGTACGCGCACCGCGTGCCTCCTGCTGCCCGGCCGCCTGGACTGCAGGCTGGGCCCGGGGGCG
CCCGCCAGCGCGCAGCCCGCGCAGCCGCCCTCGTCCTACTCGCTCCCCCTCCTGCTGTGCAAAGTGTTCA
GGTGGCCGGATCTCAGGCATTCCTCGGAAGTCAAGAGGCTGTGTTGCTGTGAATCTTACGGGAAGATCAA
CCCCGAGCTGGTGTGCTGCAACCCCCATCACCTTAGTCGACTCTGTGAACTAGAGTCTCCCCCTCCTCCT
TACTCCAGATACCCAATGGATTTTCTCAAACCAACTGCAGGCTGTCCAGATGCTGTACCTTCCTCCGCGG
AAACCGGGGGAACGAATTATCTGGCCCCTGGGGGGCTTTCAGATTCCCAACTTCTTCTGGAGCCTGGGGA
TCGGTCACACTGGTGCGTGGTGGCATACTGGGAGGAGAAGACTCGCGTGGGGAGGCTCTACTGTGTCCAA
GAGCCCTCCCTGGATATCTTCTATGATCTACCTCAGGGGAATGGCTTTTGCCTCGGACAGCTCAATTCGG
ACAACAAGAGTCAGCTGGTACAGAAAGTGCGGAGCAAGATCGGCTGTGGCATCCAGCTGACGCGGGAAGT
GGATGGCGTGTGGGTTTACAACCGCAGCAGTTACCCCATCTTCATCAAGTCCGCCACACTGGACAACCCG
GACTCCAGGACGCTGTTGGTGCACAAAGTGTTCCCTGGTTTCTCCATCAAGGCTTTTGACTATGAGAAAG
CCTACAGCCTGCAGCGGCCCAATGACCACGAGTTCATGCAGCAACCATGGACGGGTTTCACCGTGCAGAT
CAGCTTTGTGAAGGGCTGGGGCCAGTGCTACACCCGCCAGTTCATCAGCAGCTGCCCGTGCTGGCTGGAG
GTCATCTTCAACAGCCGGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_001042660
Insert Size 1281 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC137638, AAI37639
RefSeq Size 4441 bp
RefSeq ORF 1281 bp
Locus ID 17131
UniProt ID O35253
Gene Summary Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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