Kcnj10 (NM_001039484) Mouse Untagged Clone

CAT#: MC208801

Kcnj10 (untagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 10 (Kcnj10), (10ug)



  "NM_001039484" in other vectors (6)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms BIR10; BIRK-1; Kir1.2; Kir4.1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC208801 representing NM_001039484
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGTCGGTCGCTAAGGTCTATTACAGTCAGACGACTCAGACAGAGAGCCGCCCCCTAGTGGCCCCAG
GAATACGCCGGAGGAGGGTCCTCACGAAAGACGGCCGGAGCAATGTGAGAATGGAGCACATTGCTGACAA
ACGTTTCCTCTACCTCAAGGATCTATGGACGACCTTCATTGACATGCAATGGCGCTACAAGCTTCTGCTC
TTCTCTGCAACCTTTGCAGGCACGTGGTTCCTCTTTGGTGTGGTGTGGTATCTGGTAGCTGTGGCCCATG
GGGACCTGTTGGAGCTGGGACCTCCTGCCAACCACACGCCTTGTGTGGTGCAGGTGCACACGCTCACCGG
AGCCTTCCTCTTCTCCCTGGAATCCCAGACCACCATCGGCTATGGCTTCCGCTACATCAGTGAGGAATGC
CCACTGGCCATCGTGCTTCTTATTGCGCAGCTGGTGCTCACCACCATTCTGGAAATCTTCATCACAGGTA
CCTTCCTTGCAAAGATTGCCCGGCCTAAGAAGAGGGCCGAGACGATCCGCTTCAGCCAGCATGCCGTTGT
GGCTTCCCATAACGGGAAGCCTTGCCTTATGATCCGGGTTGCCAATATGCGGAAGAGTCTCCTCATTGGA
TGCCAGGTGACAGGCAAACTGCTTCAAACGCACCAGACAAAGGAGGGTGAGAATATTCGGCTCAACCAGG
TCAACGTGACTTTCCAAGTAGACACAGCCTCAGACAGCCCCTTTCTCATCCTACCCCTGACTTTCTACCA
CGTGGTAGATGAGACCAGCCCCTTAAAAGATCTCCCGCTCCGCAGTGGGGAGGGGGACTTTGAGCTGGTG
CTGATCCTGAGTGGGACAGTGGAGTCCACCAGTGCCACCTGCCAAGTTCGCACTTCCTACCTACCGGAGG
AGATCCTCTGGGGTTACGAGTTCACGCCTGCGATCTCACTGTCAGCCAGTGGCAAATACATAGCTGACTT
CAGCCTTTTCGACCAGGTTGTGAAAGTGGCATCTCCCAGTGGTCTCCGCGATAGCACCGTACGCTATGGA
GACCCCGAGAAGCTCAAGTTGGAGGAGTCATTAAGAGAGCAAGCTGAAAAGGAAGGCAGTGCCCTTAGTG
TGCGCATCAGCAACGTCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001039484
Insert Size 1140 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001039484.1, NP_001034573.1
RefSeq Size 5407 bp
RefSeq ORF 1140 bp
Locus ID 16513
UniProt ID Q9JM63
Gene Summary May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium (By similarity). In the kidney, together with KCNJ16, mediates basolateral K(+) recycling in distal tubules; this process is critical for Na(+) reabsorption at the tubules (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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