Gnao1 (NM_001113384) Mouse Untagged Clone

CAT#: MC208565

Gnao1 (untagged) - Mouse guanine nucleotide binding protein, alpha O (Gnao1), transcript variant B, (10ug)



  "NM_001113384" in other vectors (4)

CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms alphaO; AW050213; Galphao; Gnao
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC208565 representing NM_001113384
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGATGTACGCTGAGCGCAGAGGAGAGAGCCGCCCTCGAGCGGAGCAAGGCGATTGAGAAAAACCTCA
AAGAAGATGGCATCAGCGCCGCCAAAGACGTGAAATTACTCCTGCTGGGGGCTGGAGAATCAGGAAAAAG
CACCATTGTGAAGCAGATGAAGATCATCCATGAAGATGGCTTCTCTGGGGAAGACGTGAAGCAGTACAAG
CCTGTGGTCTACAGCAACACCATCCAGTCTCTGGCGGCCATTGTCCGGGCCATGGACACTTTGGGCGTGG
AGTATGGTGACAAGGAGAGGAAGACGGACTCCAAGATGGTGTGTGACGTGGTGAGTCGTATGGAAGACAC
TGAACCGTTCTCTGCAGAACTTCTTTCTGCCATGATGCGACTCTGGGGCGACTCGGGGATCCAGGAGTGC
TTCAACCGATCTCGGGAGTATCAGCTCAATGACTCTGCCAAATACTACCTGGACAGCCTGGATCGGATTG
GAGCCGGTGACTACCAGCCCACTGAGCAGGACATCCTCCGAACCAGAGTCAAAACAACTGGCATCGTAGA
AACCCACTTCACCTTCAAGAACCTCCACTTCAGGCTGTTTGACGTCGGGGGCCAGCGATCTGAACGCAAG
AAGTGGATCCACTGCTTTGAGGATGTCACGGCCATCATCTTCTGTGTCGCACTCAGCGGCTATGACCAGG
TGCTCCACGAGGACGAAACCACGAACCGCATGCACGAATCCCTGAAGCTCTTCGACAGCATCTGCAACAA
CAAGTGGTTCACAGACACATCTATTATCCTGTTTCTCAACAAGAAGGACATATTTGAGGAGAAGATCAAG
AAGTCCCCACTCACCATCTGCTTTCCTGAATACACAGGCCCCAGTGCCTTCACAGAAGCTGTGGCTCACA
TCCAAGGGCAGTATGAGAGTAAGAATAAGTCAGCTCACAAGGAAGTCTACAGCCATGTCACCTGTGCCAC
GGACACCAACAACATCCAATTCGTCTTTGATGCCGTGACAGATGTCATCATCGCCAAAAACCTACGGGGC
TGTGGACTCTACTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001113384
Insert Size 1065 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001113384.1, NP_001106855.1
RefSeq Size 6022 bp
RefSeq ORF 1065 bp
Locus ID 14681
UniProt ID P18872
Gene Summary Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Stimulated by RGS14. The G(o) protein function is not clear.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (b) includes alternate exons in the 3' coding region, compared to variant a, resulting in isoform b (also known as G0B-alpha) that has a distinct C-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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