Gnao1 (NM_001113384) Mouse Untagged Clone
CAT#: MC208565
Gnao1 (untagged) - Mouse guanine nucleotide binding protein, alpha O (Gnao1), transcript variant B, (10ug)
CNY 3,990.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | alphaO; AW050213; Galphao; Gnao |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC208565 representing NM_001113384
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGATGTACGCTGAGCGCAGAGGAGAGAGCCGCCCTCGAGCGGAGCAAGGCGATTGAGAAAAACCTCA AAGAAGATGGCATCAGCGCCGCCAAAGACGTGAAATTACTCCTGCTGGGGGCTGGAGAATCAGGAAAAAG CACCATTGTGAAGCAGATGAAGATCATCCATGAAGATGGCTTCTCTGGGGAAGACGTGAAGCAGTACAAG CCTGTGGTCTACAGCAACACCATCCAGTCTCTGGCGGCCATTGTCCGGGCCATGGACACTTTGGGCGTGG AGTATGGTGACAAGGAGAGGAAGACGGACTCCAAGATGGTGTGTGACGTGGTGAGTCGTATGGAAGACAC TGAACCGTTCTCTGCAGAACTTCTTTCTGCCATGATGCGACTCTGGGGCGACTCGGGGATCCAGGAGTGC TTCAACCGATCTCGGGAGTATCAGCTCAATGACTCTGCCAAATACTACCTGGACAGCCTGGATCGGATTG GAGCCGGTGACTACCAGCCCACTGAGCAGGACATCCTCCGAACCAGAGTCAAAACAACTGGCATCGTAGA AACCCACTTCACCTTCAAGAACCTCCACTTCAGGCTGTTTGACGTCGGGGGCCAGCGATCTGAACGCAAG AAGTGGATCCACTGCTTTGAGGATGTCACGGCCATCATCTTCTGTGTCGCACTCAGCGGCTATGACCAGG TGCTCCACGAGGACGAAACCACGAACCGCATGCACGAATCCCTGAAGCTCTTCGACAGCATCTGCAACAA CAAGTGGTTCACAGACACATCTATTATCCTGTTTCTCAACAAGAAGGACATATTTGAGGAGAAGATCAAG AAGTCCCCACTCACCATCTGCTTTCCTGAATACACAGGCCCCAGTGCCTTCACAGAAGCTGTGGCTCACA TCCAAGGGCAGTATGAGAGTAAGAATAAGTCAGCTCACAAGGAAGTCTACAGCCATGTCACCTGTGCCAC GGACACCAACAACATCCAATTCGTCTTTGATGCCGTGACAGATGTCATCATCGCCAAAAACCTACGGGGC TGTGGACTCTACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001113384 |
Insert Size | 1065 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001113384.1, NP_001106855.1 |
RefSeq Size | 6022 bp |
RefSeq ORF | 1065 bp |
Locus ID | 14681 |
UniProt ID | P18872 |
Gene Summary | Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Stimulated by RGS14. The G(o) protein function is not clear.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (b) includes alternate exons in the 3' coding region, compared to variant a, resulting in isoform b (also known as G0B-alpha) that has a distinct C-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG224064 | Gnao1 (tGFP-tagged) - Mouse guanine nucleotide binding protein alpha O (Gnao1) transcript variant B, (10ug) |
CNY 7,456.00 |
|
MR224064 | Gnao1 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein, alpha O (Gnao1), transcript variant B |
CNY 3,520.00 |
|
MR224064L3 | Lenti ORF clone of Gnao1 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein, alpha O (Gnao1), transcript variant B |
CNY 5,420.00 |
|
MR224064L4 | Lenti ORF clone of Gnao1 (mGFP-tagged) - Mouse guanine nucleotide binding protein, alpha O (Gnao1), transcript variant B |
CNY 5,420.00 |