Gfap (NM_010277) Mouse Untagged Clone

CAT#: MC208540

Gfap (untagged) - Mouse glial fibrillary acidic protein (Gfap), transcript variant 2, (10ug)



  "NM_010277" in other vectors (4)

CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
Goat Polyclonal Antibody against GFAP
    • 100 ug

CNY 5,371.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI836096
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC208540 representing NM_010277
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCGGAGACGCATCACCTCTGCGCGCCGCTCCTATGCCTCCGAGACGGTGGTCAGGGGCCTCGGTC
CTAGTCGACAACTGGGTACCATGCCACGCTTCTCCTTGTCTCGAATGACTCCTCCACTCCCTGCCAGGGT
GGACTTCTCCCTGGCCGGGGCGCTCAATGCTGGCTTCAAGGAGACACGGGCGAGCGAGCGTGCAGAGATG
ATGGAGCTCAATGACCGCTTTGCTAGCTACATCGAGAAGGTCCGCTTCCTGGAACAGCAAAACAAGGCGC
TGGCAGCTGAACTGAACCAGCTTCGAGCCAAGGAGCCCACCAAACTGGCTGATGTCTACCAGGCGGAGCT
TCGGGAGCTGCGGCTGCGGCTGGACCAGCTTACGGCCAACAGTGCCCGGCTGGAGGTGGAGAGGGACAAC
TTTGCACAGGACCTCGGCACCCTGAGGCAGAAGCTCCAAGATGAAACCAACCTGAGGCTGGAGGCAGAGA
ACAACCTGGCTGCGTATAGACAGGAGGCAGATGAAGCCACCCTGGCTCGTGTGGATTTGGAGAGAAAGGT
TGAATCGCTGGAGGAGGAGATCCAGTTCTTAAGGAAGATCTATGAGGAGGAAGTTCGAGAACTCCGGGAG
CAGCTGGCCCAACAGCAGGTCCACGTGGAGATGGATGTGGCCAAGCCAGACCTCACAGCGGCCCTGAGAG
AGATTCGCACTCAATACGAGGCAGTGGCCACCAGTAACATGCAAGAGACAGAGGAGTGGTATCGGTCTAA
GTTTGCAGACCTCACAGACGCTGCGTCCCGCAACGCAGAGCTGCTCCGCCAAGCCAAGCACGAAGCTAAC
GACTATCGCCGCCAACTGCAGGCCTTGACCTGCGATCTGGAGTCCCTGCGCGGCACGAACGAGTCCCTAG
AGCGGCAAATGCGCGAACAGGAAGAGCGCCATGCGCGGGAGTCGGCCAGTTACCAGGAGGCACTTGCTCG
GCTGGAGGAGGAGGGCCAAAGCCTCAAGGAGGAGATGGCCCGCCACCTGCAGGAGTACCAGGATCTACTC
AACGTTAAGCTAGCCCTGGACATCGAGATCGCCACCTACAGGAAATTGCTGGAGGGCGAAGAAAACCGCA
TCACCATTCCTGTACAGACTTTCTCCAACCTCCAGATCCGAGAAACCAGCCTGGACACCAAATCCGTGTC
AGAAGGCCACCTCAAGAGGAACATCGTGGTAAAGACTGTGGAGATGCGGGATGGTGAGGTCATTAAGGAC
TCGAAGCAGGAGCACAAGGACGTGGTGATGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_010277
Insert Size 1293 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_010277.3, NP_034407.2
RefSeq Size 2733 bp
RefSeq ORF 1293 bp
Locus ID 14580
UniProt ID P03995
Gene Summary GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1, which results in a protein (isoform 2) with a shorter and distinct C-terminus when compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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