Eda (NM_001177940) Mouse Untagged Clone

CAT#: MC208417

Eda (untagged) - Mouse ectodysplasin-A (Eda), transcript variant 5, (10ug)



  "NM_001177940" in other vectors (4)

CNY 3,990.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Ed1; Eda-A1; Eda-A2; EDA1; HED; Ta; tabby; Tnlg7c; XLHED
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC208417 representing NM_001177940
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCTACCCAGAGGTAGAGCGCAGGGAACCCCTGCCTGCGGCAGCGCCAAGGGAGCGGGGCAGCCAGG
GCTGCGGCTGTCGCGGGGCCCCTGCTCGGGCGGGCGAAGGGAACAGCTGCCGGCTCTTCCTGGGTTTCTT
TGGCCTCTCGCTGGCCCTCCACCTGCTGACGCTGTGCTGCTACCTAGAGTTGCGGTCCGAATTGCGGCGG
GAACGGGGAACCGAGTCCCGCCTCGGTGGCCCGGGTGCTCCTGGCACCTCTGGCACCCTAAGCAGCCCTG
GGAGCCTCGACCCGGTGGGTCCCATCACCCGCCACCTGGGGCAGCCGTCCTTTCAACAGCAGCCTTTGGA
ACCGGGAGAAGATCCACTCCCCCCTGACTCCCAGGACCGGCACCAGATGGCCCTCCTGAATTTCTTCTTT
CCTGATGAAAAGGCATATTCTGAAGAGGAAAGTAGGCGTGTTCGCCGCAATAAGAGAAGCAAAAGTGGTG
AAGGAGCAGATGGAAAGAAGGCAGGGCCACCTGGGCCCAACGGCCCCCCAGGACCTCCAGGACCTCCGGG
ACCCCAGGGACCTCCAGGGATTCCAGGAATTCCTGGGATTCCAGGAACAACTGTTATGGGACCACCTGGC
CCACCTGGCCCTCCTGGTCCTCAAGGACCCCCTGGCCTCCAAGGACCTTCTGGTGCTGCTGATAAAACTG
GAACTCGGGAAAATCAGCCAGCTGTGGTGCATCTGCAGGGCCAAGGGTCAGCAATTCAAGTCAAAAATGA
TCTTTCAGGTGGAGTGCTCAATGACTGGTCTCGCATCACTATGAACCCTAAGGTGTTTAAACTACATCCC
CGCAGCGGGGAGCTGGAGGTACTGGTGGACGGCACCTACTTCATCTATAGTCAGGTAGAAGTCTACTACA
TCAACTTCACTGACTTTGCCAGCTACGAGGTGGTGGTGGATGAGAAGCCCTTCCTGCAGTGCACCCGCAG
CATTGAGACAGGGAAGACCAACTACAACACTTGCTATACTGCAGGCGTGTGCCTCCTCAAGGCCAGGCAG
AAAATCGCCGTGAAGATGGTGCACGCTGACATCTCTATCAATATGAGCAAGCACACCACCTTCTTCGGGG
CCATCAGGCTGGGCGAAGCCCCTGCATCCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001177940
Insert Size 1152 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001177940.1, NP_001171411.1
RefSeq Size 4931 bp
RefSeq ORF 1152 bp
Locus ID 13607
Gene Summary Cytokine which is involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Functions as a ligand activating the DEATH-domain containing receptors EDAR and EDA2R. Isoform TAA binds only to the receptor EDAR, while isoform TA-A2 binds exclusively to the receptor EDA2R (By similarity). May also play a role in cell adhesion (PubMed:10534613).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (5) lacks an in-frame exon in the CDS, as compared to variant 1. The resulting isoform (5) lacks an internal segment, as compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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