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Eda (NM_001177937) Mouse Untagged Clone
CAT＃: MC208415
Eda (untagged) - Mouse ectodysplasin-A (Eda), transcript variant 2, (10ug)
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Ed1; Eda-A1; Eda-A2; EDA1; HED; Ta; tabby; Tnlg7c; XLHED |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC208415 representing NM_001177937
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTACCCAGAGGTAGAGCGCAGGGAACCCCTGCCTGCGGCAGCGCCAAGGGAGCGGGGCAGCCAGG GCTGCGGCTGTCGCGGGGCCCCTGCTCGGGCGGGCGAAGGGAACAGCTGCCGGCTCTTCCTGGGTTTCTT TGGCCTCTCGCTGGCCCTCCACCTGCTGACGCTGTGCTGCTACCTAGAGTTGCGGTCCGAATTGCGGCGG GAACGGGGAACCGAGTCCCGCCTCGGTGGCCCGGGTGCTCCTGGCACCTCTGGCACCCTAAGCAGCCCTG GGAGCCTCGACCCGGTGGGTCCCATCACCCGCCACCTGGGGCAGCCGTCCTTTCAACAGCAGCCTTTGGA ACCGGGAGAAGATCCACTCCCCCCTGACTCCCAGGACCGGCACCAGATGGCCCTCCTGAATTTCTTCTTT CCTGATGAAAAGGCATATTCTGAAGAGGAAAGTAGGCGTGTTCGCCGCAATAAGAGAAGCAAAAGTGGTG AAGGAGCAGATGGTCCTGTTAAAAACAAGAAAAAGGGAAAGAAGGCAGGGCCACCTGGGCCCAACGGCCC CCCAGGACCTCCAGGACCTCCGGGACCCCAGGGACCTCCAGGGATTCCAGGAATTCCTGGGATTCCAGGA ACAACTGTTATGGGACCACCTGGCCCACCTGGCCCTCCTGGTCCTCAAGGACCCCCTGGCCTCCAAGGAC CTTCTGGTGCTGCTGATAAAACTGGAACTCGGGAAAATCAGCCAGCTGTGGTGCATCTGCAGGGCCAAGG GTCAGCAATTCAAGTCAAAAATGATCTTTCAGGTGGAGTGCTCAATGACTGGTCTCGCATCACTATGAAC CCTAAGGTGTTTAAACTACATCCCCGCAGCGGGGAGCTGGAGGTACTGGTGGACGGCACCTACTTCATCT ATAGTCAGGTCTACTACATCAACTTCACTGACTTTGCCAGCTACGAGGTGGTGGTGGATGAGAAGCCCTT CCTGCAGTGCACCCGCAGCATTGAGACAGGGAAGACCAACTACAACACTTGCTATACTGCAGGCGTGTGC CTCCTCAAGGCCAGGCAGAAAATCGCCGTGAAGATGGTGCACGCTGACATCTCTATCAATATGAGCAAGC ACACCACCTTCTTCGGGGCCATCAGGCTGGGCGAAGCCCCTGCATCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001177937 |
| Insert Size | 1170 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001177937.1, NP_001171408.1 |
| RefSeq Size | 4949 bp |
| RefSeq ORF | 1170 bp |
| Locus ID | 13607 |
| UniProt ID | O54693 |
| Gene Summary | Cytokine which is involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Functions as a ligand activating the DEATH-domain containing receptors EDAR and EDA2R. Isoform TAA binds only to the receptor EDAR, while isoform TA-A2 binds exclusively to the receptor EDA2R (By similarity). May also play a role in cell adhesion (PubMed:10534613).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame small segment in the CDS, as compared to variant 1. The resulting isoform (2) lacks two internal aa, as compared to isoform 1. |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG223486 | Eda (tGFP-tagged) - Mouse ectodysplasin-A (Eda) transcript variant 2, (10ug) |
CNY 7,456.00 |
|
| MR223486 | Eda (Myc-DDK-tagged) - Mouse ectodysplasin-A (Eda), transcript variant 2 |
CNY 3,900.00 |
|
| MR223486L3 | Lenti ORF clone of Eda (Myc-DDK-tagged) - Mouse ectodysplasin-A (Eda), transcript variant 2 |
CNY 5,800.00 |
|
| MR223486L4 | Lenti ORF clone of Eda (mGFP-tagged) - Mouse ectodysplasin-A (Eda), transcript variant 2 |
CNY 5,800.00 |
