Aga (NM_001005847) Mouse Untagged Clone
CAT#: MC208116
Aga (untagged) - Mouse aspartylglucosaminidase (Aga), transcript variant 1, (10ug)
CNY 3,990.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | AW060726 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC208116 representing NM_001005847
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGGAAGTCGAATCTGTCTCTGCTTCTCCTACTGCTGGTCCTGGGCATGCCCCTGGTGCGGGGCT CCAGCCCTCTGCCCCTGGTCGTCAACACTTGGCCTTTTAAGAATGCCACTGAAGCAGCGTGGTGGACATT GCTATCTGGAGGTTCTGCCCTGGATGCAGTGGAGAACGGCTGTGCTGTGTGTGAGAAGGAGCAGTGTGAT GGGACTGTAGGCTTTGGAGGAAGTCCTGATGAAGGTGGCGAAACCACCCTGGATGCCATGATAATGGATG GCACTGCCATGGATGTGGGAGCAGTGGGAGGCCTTAGAAGAATTAAAAACGCGATTGGCGTGGCGCGGAG AGTCCTGGAGCATACCACACACACGCTTTTAGTGGGGGACTCAGCCACCAAGTTTGCTGAAAGTATGGGG TTTACTAATGAGGACTTGTCTACCAAAACCTCAAGAGATCTTCATTCAGATTGGCTTTCTCGAAATTGCC AGCCAAATTATTGGAGAAATGTTATTCCAGATCCCTCAAAATACTGTGGACCCTACAAACCATCTGGTTT CTTAAAGCAGAGTATTTCTCCCCACAAAGAAGAAGTGGATATCCACAGCCATGATACTATTGGCATGGTT GTAATCCATAAGACGGGACATACTGCTGCTGGCACATCCACAAATGGTATAAAATTCAAAATACCTGGTC GTGTAGGGGATTCACCAATCCCTGGAGCCGGAGCCTATGCTGATGACACGGCTGGAGCAGCTGCAGCCAC TGGCGATGGTGACACACTCCTGCGCTTTCTGCCGAGCTACCAAGCTGTAGAATATATGAGAGGAGGAGAT GACCCAGCCATAGCTTGCCAAAAAGTGATTTTAAGAATTCAGAAATACTATCCAAACTTCTTTGGAGCGG TCATATGTGCCAGTGTGAACGGAAGTTATGGTGCTGCTTGCAACAAACTTCCAACATTTACACAATTTAG TTTCATGGTTTCTAATTCTTTACACAATGAGCCAACCGAAAAAAAAGTAGACTGCATCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001005847 |
Insert Size | 1041 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001005847.2, NP_001005847.1 |
RefSeq Size | 1307 bp |
RefSeq ORF | 1041 bp |
Locus ID | 11593 |
UniProt ID | Q64191 |
Gene Summary | This gene encodes an amidase enzyme that participates in the breakdown of glycoproteins in the cell. The encoded protein undergoes proteolytic processing to generate a mature enzyme. Mice lacking the encoded protein exhibit accumulation of aspartylglucosamine along with lysosomal vacuolization, axonal swelling in the gracile nucleus and impaired neuromotor coordination. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Oct 2015] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG224201 | Aga (tGFP-tagged) - Mouse aspartylglucosaminidase (Aga), (10ug) |
CNY 3,140.00 |
|
MR224201 | Aga (Myc-DDK-tagged) - Mouse aspartylglucosaminidase (Aga), transcript variant 1 |
CNY 3,656.00 |
|
MR224201L3 | Lenti ORF clone of Aga (Myc-DDK-tagged) - Mouse aspartylglucosaminidase (Aga), transcript variant 1 |
CNY 4,750.00 |
|
MR224201L4 | Lenti ORF clone of Aga (mGFP-tagged) - Mouse aspartylglucosaminidase (Aga), transcript variant 1 |
CNY 4,750.00 |