United States
Germany
Japan
United Kingdom
China
Rrm2b (NM_199476) Mouse Untagged Clone
CAT＃: MC207972
Rrm2b (untagged) - Mouse ribonucleotide reductase M2 B (TP53 inducible) (Rrm2b), (10ug)
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | p53R2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC207972 representing NM_199476
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGACCCGGAAAGGCCGGAAGCGGCCAGGCCGGAGAAGGGTGAGCAATTGTGTTCGGAGACAGAAG AAAATGTGGTGAGGTCAAATGAAGAGCCACTCCTAAGAAAGAGTTCCCGACGGTTCGTCATCTTTCCAAT CCAGTATCCTGATATCTGGAGAATGTACAAGCAAGCACAGGCATCTTTCTGGACAGCAGAGGAGGTTGAC TTGTCAAAGGACCTCCCTCACTGGAACAAGCTTAAATCCGACGAGAAGTATTTTATCTCCCACATCTTAG CCTTTTTTGCAGCCAGTGATGGAATTGTAAATGAAAACTTGGTGGAGCGTTTTAGTCAGGAGGTGCAGGT TCCAGAGGCTCGCTGCTTCTATGGCTTTCAGATTCTCATCGAGAATGTTCACTCAGAGATGTACAGTTTA CTAATAGACACTTACATCAGAGATCCAAAGAAAAGGGAATTTTTATTTAATGCAATTGAAACAATGCCAT ATGTTAAGAAAAAAGCGGACTGGGCTCTGCGCTGGATAGCAGATAGGAAATCTACTTTTGGGGAAAGAGT GGTGGCCTTTGCTGCTGTGGAAGGAATTTTCTTCTCTGGATCATTTGCTGCAATATTCTGGCTAAAGAAG AGAGGCCTCATGCCTGGACTGACTTTTTCAAATGAACTGATCAGCAGGGATGAAGGACTTCACTGTGACT TTGCCTGCCTGATGTTTCAGTACTTGGTAAACAAGCCTTCAGAAGATAGAGTGAGGGAAATCATTGCTGA TGCTGTTCAAATCGAGCAGGAGTTTTTAACAGAAGCCTTGCCTGTTGGCCTCATTGGAATGAACTGTGTT TTGATGAAACAGTACATTGAGTTTGTAGCTGACAGATTACTTGGAGAACTTGGATTCTCAAAGATTTTTC AGGCAGAAAATCCCTTTGATTTTATGGAGAACATTTCATTAGAAGGGAAAACAAATTTCTTTGAGAAACG AGTTTCTGAGTATCAGCGATTTGCAGTCATGGCCGAAACCACAGATAATGTCTTCACCTTGGATGCAGAT TTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_199476 |
| Insert Size | 1056 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC058103, AAH58103 |
| RefSeq Size | 4532 bp |
| RefSeq ORF | 1056 bp |
| Locus ID | 382985 |
| UniProt ID | Q6PEE3 |
| Gene Summary | Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG205344 | Rrm2b (tGFP-tagged) - Mouse ribonucleotide reductase M2 B (TP53 inducible) (Rrm2b) |
CNY 4,370.00 |
|
| MR205344 | Rrm2b (Myc-DDK-tagged) - Mouse ribonucleotide reductase M2 B (TP53 inducible) (Rrm2b) |
CNY 5,488.00 |
|
| MR205344L3 | Lenti ORF clone of Rrm2b (Myc-DDK-tagged) - Mouse ribonucleotide reductase M2 B (TP53 inducible) (Rrm2b) |
CNY 5,890.00 |
|
| MR205344L4 | Lenti ORF clone of Rrm2b (mGFP-tagged) - Mouse ribonucleotide reductase M2 B (TP53 inducible) (Rrm2b) |
CNY 5,890.00 |
