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B3gat1 (NM_029792) Mouse Untagged Clone
CAT＃: MC207764
B3gat1 (untagged) - Mouse beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) (B3gat1), (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 0710007K08Rik; AI846286; GlcAT-P; Glcatp; Hnk1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC207764 representing NM_029792
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGAAGAGACGGGACATCCTTGCGATTGTCCTCATCGTGCTTCCCTGGACACTGCTCATCACCGTCT GGCACCAGAGCAGCCTCGCACCTCTGCTTGCTGTGCACAAGGATGAGGGAAGTGACCCCCGCCATGAGGC ACCACCTGGTGCGGACCCTAGGGAGTACTGCATGTCCGACCGTGACATCGTGGAAGTGGTGCGCACAGAG TACGTGTACACGAGGCCGCCACCCTGGTCCGACACGCTGCCCACCATCCATGTGGTGACGCCCACCTACA GTAGACCGGTGCAGAAGGCAGAGCTGACGCGAATGGCCAACACACTACTGCATGTGCCCAACCTTCACTG GCTAGTGGTGGAGGATGCTCCACGCAGGACGCCCCTCACCGCGCGCTTGCTGCGCGACACTGGCCTCAAC TACACACACCTGCACGTAGAGACACCACGCAACTATAAGCTGCGAGGTGATGCCCGAGACCCTCGCATCC CACGTGGCACCATGCAGCGCAACCTGGCACTGCGCTGGCTGCGGGAGACCTTCCCACGGAACTCTACTCA GCCAGGCGTAGTGTACTTCGCGGATGATGACAACACGTACAGTCTGGAGCTCTTTGAAGAGATGCGCAGC ACAAGAAGGGTGTCCGTGTGGCCTGTGGCCTTTGTTGGTGGCCTTCGGTATGAGGCCCCACGGGTGAATG GGGCAGGGAAAGTGGTTGGCTGGAAGACAGTCTTCGACCCCCACCGGCCCTTTGCAATAGACATGGCTGG ATTTGCTGTCAACCTCCGGCTCATCTTGCAGCGAAGTCAAGCCTACTTTAAGCTACGTGGTGTGAAGGGA GGCTACCAGGAAAGCAGTCTCCTTCGAGAACTTGTCACCCTCAATGACCTGGAGCCCAAGGCAGCAAACT GTACCAAGATCTTGGTCTGGCATACACGAACAGAGAAGCCAGTGCTGGTCAATGAGGGGAAGAAGGGCTT CACCGACCCCTCAGTGGAGATCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_029792 |
| Insert Size | 1005 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_029792.1, NP_084068.1 |
| RefSeq Size | 3474 bp |
| RefSeq ORF | 1005 bp |
| Locus ID | 76898 |
| UniProt ID | Q9CW73 |
| Gene Summary | Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and uses a downstream start codon compared to variant 1. It encodes isoform 2, which has a shorter N-terminus compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG204943 | B3gat1 (tGFP-tagged) - Mouse beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) (B3gat1) |
CNY 3,710.00 |
|
| MR204943 | B3gat1 (Myc-DDK-tagged) - Mouse beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) (B3gat1) |
CNY 3,656.00 |
|
| MR204943L3 | Lenti ORF clone of B3gat1 (Myc-DDK-tagged) - Mouse beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) (B3gat1) |
CNY 5,230.00 |
|
| MR204943L4 | Lenti ORF clone of B3gat1 (mGFP-tagged) - Mouse beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) (B3gat1) |
CNY 5,230.00 |
