Plekhf1 (NM_024413) Mouse Untagged Clone

CAT#: MC207721

Plekhf1 (untagged) - Mouse pleckstrin homology domain containing, family F (with FYVE domain) member 1 (Plekhf1), (10ug)



  "NM_024413" in other vectors (4)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1810013P09Rik; APPD; LAPF; PHAFIN1; ZFYVE15
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC207721 representing NM_024413
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGACCACTTGGCAAACACGGAGATCAACAGCCAGCGGATTGCAGCAGTGGAGAGCTGTTTTGGGG
CATCGGGGCAACCGCTGGCCCTGCCCGGCCGTGTGCTCCTAGGCGAGGGGGTACTGACCAAGGAGTGCCG
CAAGAAGGCCAAGCCACGCATCTTCTTTCTTTTCAACGACATCCTGGTGTATGGCAGTATTGTGCTTAGC
AAGCGCAAGTACCGCAGCCAGCATATTATTCCCCTAGAAGAGGTAACATTGGAGCCACTGCCCGAGACCC
TGCAGGCCAAGAACCGATGGATGATCAAGACAGCCAAGAAGTCCTTTGTGGTGTCGGCAGCCTCCACCAC
GGAGCGCCAGGAATGGATAAGCCACATCGAAGAGTGTGTGCGGAGGCAGCTACTGGCCACAGGCCGCCAG
CCCACCACGGAGCACGCAGCTCCCTGGATCCCGGACAAGGCCACGGACATTTGCATGCGCTGCACGCAGA
CGCGCTTCTCCGCACTCACCCGGCGTCACCACTGCCGCAAATGCGGGTTTGTGGTCTGTGCTGAGTGTTC
CAGGGAGCGTTTCCTGTTGCCACGTCTCTCTCCCAAGCCCCTTCGCGTCTGCAGCCTGTGCTACCGAGAG
CTGGCCGCCCAGAAGCTGAGGGAGGAGGCCAGGGAGGGGATCGGAGGCTCTCCACCGCAGCTGTCCCACC
TAGGCGGCACTGTCTGTGGTGCATCCAGTGGCGATGACGATGACTCCGATGAGGACAGAGAGGGCAATGG
AGATGGTGACTGGCCCACCCAGGTGGAGTTCTATGCTTCTGGTGTCTCCTGGTCAGCCTTCCATAGTTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_024413
Insert Size 840 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_024413.2, NP_077724.2
RefSeq Size 1559 bp
RefSeq ORF 840 bp
Locus ID 72287
UniProt ID Q3TB82
Gene Summary May induce apoptosis through the lysosomal-mitochondrial pathway. Translocates to the lysosome initiating the permeabilization of lysosomal membrane (LMP) and resulting in the release of CTSD and CTSL to the cytoplasm. Triggers the caspase-independent apoptosis by altering mitochondrial membrane permeabilization (MMP) resulting in the release of PDCD8 (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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