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Wnt5a (NM_009524) Mouse Untagged Clone
CAT＃: MC207444
Wnt5a (untagged) - Mouse wingless-related MMTV integration site 5A (Wnt5a), (10ug)
CNY 3,656.00
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 8030457G12Rik; Wnt-5a |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC207444 representing NM_009524
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAAGCCCATTGGAATATTAAGCCCGGGAGTGGCTTTGGGGACCGCTGGAGGTGCCATGTCTTCCA AGTTCTTCCTAATGGCTTTGGCCACGTTTTTCTCCTTCGCCCAGGTTGTTATAGAAGCTAATTCTTGGTG GTCTCTAGGTATGAATAACCCTGTTCAGATGTCAGAAGTATATATCATAGGTGCACAGCCTCTCTGCAGC CAACTGGCAGGACTTTCTCAAGGACAGAAGAAACTCTGCCACTTGTATCAGGACCACATGCAGTACATTG GAGAAGGTGCGAAGACAGGCATCAAGGAATGCCAGTACCAGTTCCGGCATCGGAGATGGAACTGCAGCAC AGTGGACAATACTTCTGTCTTTGGCAGGGTGATGCAAATAGGCAGCCGAGAGACGGCCTTCACGTACGCG GTGAGCGCAGCTGGGGTGGTGAACGCCATGAGCCGAGCATGCCGGGAGGGCGAGCTGTCTACCTGTGGCT GCAGCCGCGCTGCGCGCCCCAAGGACCTGCCTCGGGACTGGTTGTGGGGCGGCTGCGGAGACAACATCGA CTATGGCTACCGCTTCGCCAAGGAGTTCGTGGACGCTAGAGAAAGGGAACGAATCCACGCTAAGGGTTCC TATGAGAGCGCACGCATCCTCATGAACTTACACAACAATGAAGCAGGCCGTAGGACAGTATACAACCTGG CAGATGTAGCCTGTAAGTGTCATGGAGTGTCTGGCTCCTGTAGCCTCAAGACGTGCTGGCTGCAGCTGGC GGACTTCCGGAAGGTGGGCGATGCCCTCAAGGAGAAGTATGATAGCGCGGCGGCCATGAGGCTCAACAGC CGGGGCAAGCTGGTGCAGGTCAACAGCCGCTTCAACTCCCCGACCACGCAGGACCTGGTCTACATCGACC CCAGTCCGGACTACTGTGTGCGCAACGAGAGCACTGGCTCGCTGGGCACGCAGGGACGCCTGTGCAACAA GACCTCAGAGGGGATGGACGGCTGCGAGCTCATGTGCTGTGGGCGTGGCTATGACCAGTTTAAGACAGTG CAGACCGAACGCTGTCATTGCAAGTTTCACTGGTGCTGCTATGTCAAATGCAAGAAGTGCACGGAGATTG TGGATCAGTTCGTGTGCAAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_009524 |
| Insert Size | 1143 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC018425, AAH18425 |
| RefSeq Size | 4354 bp |
| RefSeq ORF | 1143 bp |
| Locus ID | 22418 |
| UniProt ID | P22725 |
| Gene Summary | Ligand for members of the frizzled family of seven transmembrane receptors (PubMed:17117926). Can activate or inhibit canonical Wnt signaling, depending on receptor context (PubMed:16602827). In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression (PubMed:16602827). Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration (PubMed:17117926). Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells (By similarity). Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle (PubMed:10021340). Inhibits type II collagen expression in chondrocytes (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG205939 | Wnt5a (tGFP-tagged) - Mouse wingless-related MMTV integration site 5A (Wnt5a) |
CNY 3,710.00 |
|
| MR205939 | Wnt5a (Myc-DDK-tagged) - Mouse wingless-related MMTV integration site 5A (Wnt5a) |
CNY 3,656.00 |
|
| MR205939L3 | Lenti ORF clone of Wnt5a (Myc-DDK-tagged) - Mouse wingless-related MMTV integration site 5A (Wnt5a) |
CNY 6,056.00 |
|
| MR205939L4 | Lenti ORF clone of Wnt5a (mGFP-tagged) - Mouse wingless-related MMTV integration site 5A (Wnt5a) |
CNY 6,056.00 |
