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Tbp (NM_013684) Mouse Untagged Clone
CAT＃: MC207415
Tbp (untagged) - Mouse TATA box binding protein (Tbp), (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Gtf2d; GTF2D1; SCA17; TFIID |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC207415 representing NM_013684
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCAGAACAACAGCCTTCCACCTTATGCTCAGGGCTTGGCCTCCCCACAGGGCGCCATGACTCCTG GAATTCCCATCTTTAGTCCAATGATGCCTTACGGCACAGGACTTACTCCACAGCCTATTCAGAACACCAA CAGTCTCTCTATTTTGGAAGAGCAACAAAGACAGCAGCAGCAACAGCAACAGCAGCAGCAACAACAGCAG GCAGTAGCAACTGCAGCAGCCTCAGTACAGCAATCAACATCTCAGCAACCCACACAGGGTGCCTCAGGCC AGACCCCACAACTCTTCCATTCTCAAACTCTGACCACTGCACCGTTGCCAGGCACCACCCCCTTGTACCC TTCACCAATGACTCCTATGACCCCTATCACTCCTGCCACACCAGCTTCTGAGAGCTCTGGAATTGTACCG CAGCTTCAAAATATTGTATCTACCGTGAATCTTGGCTGTAAACTTGACCTAAAGACCATTGCACTTCGTG CAAGAAATGCTGAATATAATCCCAAGCGATTTGCTGCAGTCATCATGAGAATAAGAGAGCCACGGACAAC TGCGTTGATTTTCAGTTCTGGAAAAATGGTGTGCACAGGAGCCAAGAGTGAAGAACAATCCAGACTAGCA GCAAGAAAATATGCTAGAGTTGTGCAGAAGTTGGGCTTCCCAGCTAAGTTCTTAGACTTCAAGATCCAGA ACATGGTGGGGAGCTGTGATGTGAAGTTCCCTATAAGGCTGGAAGGCCTTGTGCTGACCCACCAGCAGTT CAGTAGCTATGAGCCAGAATTATTTCCTGGATTAATCTACAGAATGATCAAACCCAGAATTGTTCTCCTT ATTTTTGTTTCTGGAAAAGTTGTATTAACAGGTGCTAAAGTTAGAGCAGAGATTTATGAAGCATTTGAAA ACATCTACCCCATCTTAAAGGGATTCAGGAAGACCACATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_013684 |
| Insert Size | 951 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_013684.3, NP_038712.3 |
| RefSeq Size | 1842 bp |
| RefSeq ORF | 951 bp |
| Locus ID | 21374 |
| UniProt ID | P29037 |
| Gene Summary | General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG204589 | Tbp (tGFP-tagged) - Mouse TATA box binding protein (Tbp) |
CNY 2,850.00 |
|
| MR204589 | Tbp (Myc-DDK-tagged) - Mouse TATA box binding protein (Tbp) |
CNY 2,400.00 |
|
| MR204589L3 | Lenti ORF clone of Tbp (Myc-DDK-tagged) - Mouse TATA box binding protein (Tbp) |
CNY 4,750.00 |
|
| MR204589L4 | Lenti ORF clone of Tbp (mGFP-tagged) - Mouse TATA box binding protein (Tbp) |
CNY 4,750.00 |
