St8sia3 (NM_009182) Mouse Untagged Clone

CAT#: MC207397

St8sia3 (untagged) - Mouse ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3 (St8sia3), (10ug)



  "NM_009182" in other vectors (4)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI847333; Siat8c; ST8SiaIII
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC207397 representing NM_009182
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAAATTGCAAAATGGCCCGAGTCGCCAGTGTGCTAGGGCTGGTCATGCTCAGCGTGGCCCTGCTGA
TTTTATCGCTTATCAGCTACGTGTCTCTGAAAAAGGAGAACATCTTCACCACTCCCAAGTACGCCAGCCC
GGGGGCGCCCCGAATGTACATGTTCCACGCGGGATTCCGGTCACAGTTTGCACTGAAGTTTCTAGACCAG
TCATTTGTGCCCATTACGAATTCTCTCACCCATGAACTCCAAGAGAAACCTTCTAAATGGACATTTAATC
GGACAGCGTTTTTACATCAAAGGCAAGAAATTCTTCAGCATGTCGATGTAATAAAAAATTTTTCTTTGAC
CAAGAGTAGTGTTCGGATTGGACAACTAATGCATTATGATTATTCCAGCCATAAATATGTCTTCTCGATT
AGCAATAACTTCCGGTCCCTGCTCCCAGATGTGTCGCCCATTATGAATAAGCGTTATAATGTTTGTGCTG
TGGTTGGAAACAGTGGAATCTTGACAGGGAGTCAGTGTGGACAAGAAATAGATAAATCAGATTTTGTTTT
TCGATGCAATTTTGCCCCGACAGAGGCTTTCCACAAAGATGTTGGAAGGAAAACCAACCTCACAACCTTC
AATCCGAGCATCTTAGAGAAATATTACAACAATCTTTTAACCATTCAGGACCGTAACAACTTCTTCCTCA
GTTTAAAAAAGCTTGATGGGGCCATACTTTGGATCCCTGCATTTTTCTTCCACACTTCTGCAACTGTAAC
GAGAACGCTAGTGGATTTTTTTGTTGAGCACAGAGGTCAGTTAAAGGTCCAGTTGGCTTGGCCTGGAAAT
ATCATGCAACATGTCAACAGGTACTGGAAAAACAAACACCTGTCACCCAAACGACTGAGCACAGGTATCC
TAATGTATACTCTTGCATCTGCAATATGTGAAGAGATCCACTTGTACGGTTTCTGGCCCTTTGGATTTGA
CCCCAACACCAGGGAGGATCTGCCCTACCACTACTATGACAAAAAAGGAACCAAATTTACCACCAAGTGG
CAGGAGTCTCACCAGCTGCCTGCTGAGTTTCAGCTGCTCTATCGAATGCATGGGGAAGGGCTCACGAAGC
TCACTCTGTCACACTGTGCCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_009182
Insert Size 1143 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_009182.3, NP_033208.2
RefSeq Size 6306 bp
RefSeq ORF 1143 bp
Locus ID 20451
UniProt ID Q64689
Gene Summary Catalyzes the transfer of sialic acid from a CMP-linked sialic acid donor onto the terminal sialic acid of an acceptor through alpha-2,8-linkages. Is active with alpha-2,3-linked, alpha-2,6-linked and alpha-2,8-linked sialic acid of N-linked oligosaccharides of glycoproteins and glycolipids. Displays preference for substrates with alpha-2,3-linked terminal sialic acid. It can form polysialic acid in vitro directly on alpha-2,3-, alpha-2,6-, or alpha-2,8-linked sialic acid.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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