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Ier5 (NM_010500) Mouse Untagged Clone
CAT＃: MC207308
Ier5 (untagged) - Mouse immediate early response 5 (Ier5), (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC207308 representing NM_010500
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTCAAGCTGGAGGCTCACCGCATCGTCAGCATCTCCCTGGGCAAGATCTACAACTCGCGGGTCC AGCGCGGCGGCATCAAGCTGCACAAGAACCTCTTGGTGTCGTTGGTGCTGCGCAGCGCCCGCCAAGTCTA CCTGAGTGACCCCTGCCCTGGCCTCTACCTGGCTGGCCCTGCGGGGACCCCGGCGGTGCCGCCGCCTCAG CAGCCTGGAGAGCCTGTAGCCGGGCCACCCTCCGGCTGGGGAGAGCCGCCCCCGCCGGTCGCCCGTGCCG CCTGGCCGGAGCCCGAGCCTCAGCCGCAGCCTCAGCCTCAGCGTCCCTCCGTGTGCCACACGCCCGGAGC GGGAAGCTCAGAACCGGTGGCCGCGGTGGCCGGATCGGGGGAAGCTCTCCGGGGCGGAGAGGAGGACTCG GCGGCAGCTGCTTGGGGCCGCGTGGAGCGTCCGCGCGCGGCGTCTTCAGGAGGTGGCTCGGACGCGTGCC CCGAGGGGCCCCGGGCAGTGCGCCGCCCGTGCGGCTGTCCCCCGGCCGTGGAGGAGCGGTCCTCCGAGGA CGGGTCCCCCGCGCCACCCGCCCCGTGTCCCAGGAAGCGTGGCGCAGCGGGAGTGGGTGGCGGCCGCGCG AGCTGCTCGGCACCCGGCTCTACCCCTCTCAAGAAGCCGCGCCGGAACTCGGAGGAGCAGCCAGTCACCG GCGAGGACGACACCGACGAGGAGATGGAGACCGGGAACGTGGCAAACCTCATCAGCATCTTCGGGTCTAG CTTCTCGGGACTCCTACGGAAAAGCCCCGCAGGCGGCCGAGAGGAAGAGGAGGCGGAGGAGGGCGGCCCG GAAGCCGCCGAGCCGGGGCAGATCTGCTGCGACAAGCCGGTGCTGAGAGACATGAGCCCCTGGAGCACAG CCATCGTGGCCTTCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_010500 |
| Insert Size | 927 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_010500.2, NP_034630.1 |
| RefSeq Size | 3272 bp |
| RefSeq ORF | 927 bp |
| Locus ID | 15939 |
| UniProt ID | O89113 |
| Gene Summary | Plays a role as a transcription factor. Mediates positive transcriptional regulation of several chaperone gene during the heat shock response in a HSF1-dependent manner. Mediates negative transcriptional regulation of CDC25B expression. Plays a role in the dephosphorylation of the heat shock factor HSF1 and ribosomal protein S6 kinase (S6K) by the protein phosphatase PP2A. Involved in the regulation of cell proliferation and resistance to thermal stress. Involved in the cell cycle checkpoint and survival in response to ionizing radiation. Associates with chromatin to the CDC25B promoter.[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG204347 | Ier5 (tGFP-tagged) - Mouse immediate early response 5 (Ier5) |
CNY 2,850.00 |
|
| MR204347 | Ier5 (Myc-DDK-tagged) - Mouse immediate early response 5 (Ier5) |
CNY 2,400.00 |
|
| MR204347L3 | Lenti ORF clone of Ier5 (Myc-DDK-tagged) - Mouse immediate early response 5 (Ier5) |
CNY 4,750.00 |
|
| MR204347L4 | Lenti ORF clone of Ier5 (mGFP-tagged) - Mouse immediate early response 5 (Ier5) |
CNY 4,750.00 |
