Glul (NM_008131) Mouse Untagged Clone

CAT#: MC207287

Glul (untagged) - Mouse glutamate-ammonia ligase (glutamine synthetase) (Glul), (10ug)



  "NM_008131" in other vectors (3)

CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Glns; GS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NCBI ORF sequence for NM_008131, the custom clone sequence may differ by one or more nucleotides


ATGGCCACCTCAGCAAGTTCCCACTTGAACAAAGGCATCAAGCAAATGTACATGTCCCTGCCCCAGGGTG
AGAAAGTCCAAGCCATGTATATCTGGGTTGATGGTACCGGAGAAGGACTGCGCTGCAAGACCCGTACCCT
GGACTGTGAGCCCAAGTGTGTGGAAGAGTTACCTGAGTGGAACTTTGATGGCTCTAGTACCTTTCAGTCT
GAAGGCTCCAACAGCGACATGTACCTCCATCCTGTTGCCATGTTTCGAGACCCCTTCCGCAAAGACCCCA
ACAAGCTGGTGCTATGTGAAGTTTTCAAGTATAACCGGAAGCCTGCAGAGACCAACTTGAGGCACATCTG
TAAACGGATAATGGACATGGTGAGCAACCAGCACCCCTGGTTTGGAATGGAGCAGGAATATACTCTTATG
GGAACAGACGGCCACCCATTTGGTTGGCCTTCCAATGGCTTCCCTGGACCCCAAGGCCCGTATTACTGCG
GTGTGGGAGCAGACAAGGCCTACGGCAGGGACATCGTGGAGGCTCACTACCGGGCCTGCTTGTATGCTGG
AGTCAAGATCACGGGGACAAATGCGGAGGTTATGCCTGCCCAGTGGGAATTCCAGATAGGACCCTGTGAG
GGGATCCGAATGGGAGATCATCTTTGGATAGCCCGTTTTATCTTGCATCGGGTGTGCGAAGACTTTGGGG
TGATAGCAACCTTTGACCCCAAGCCCATTCCAGGGAACTGGAATGGTGCAGGCTGCCATACCAACTTCAG
CACCAAGGCCATGCGGGAGGAGAATGGTCTGAAGTGCATTGAGGAGGCCATTGACAAACTGAGCAAGAGG
CACCAGTACCACATCCGCGCCTACGATCCCAAGGGGGGCCTGGACAACGCCCGGCGTCTGACTGGATTCC
ACGAAACCTCCAACATCAACGACTTTTCTGCCGGTGTTGCCAACCGCGGTGCCAGTATCCGCATTCCCCG
GACTGTCGGCCAGGAGAAGAAGGGCTACTTTGAAGACCGTCGGCCTTCTGCCAATTGTGACCCCTATGCG
GTGACAGAAGCCATCGTCCGCACGTGTCTCCTCAACGAAACAGGCGACGAACCCTTCCAATACAAGAACT
:AA


Restriction Sites SgfI-MluI     
ACCN NM_008131
Insert Size 1122 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC015086, AAH15086
RefSeq Size 1435 bp
RefSeq ORF 1122 bp
Locus ID 14645
UniProt ID P15105
Gene Summary Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine (By similarity). Its role depends on tissue localization: in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia (PubMed:25870278). Essential for proliferation of fetal skin fibroblasts (By similarity). Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development (PubMed:30158707). Involved in angiogenesis by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation (By similarity). May act as a palmitoyltransferase for RHOJ: able to autopalmitoylate and then transfer the palmitoyl group to RHOJ (By similarity). Plays a role in ribosomal 40S subunit biogenesis (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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