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Asah1 (NM_019734) Mouse Untagged Clone
CAT＃: MC207233
Asah1 (untagged) - Mouse N-acylsphingosine amidohydrolase 1 (Asah1), (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2310081N20Rik; AC; Asah |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC207233 representing NM_019734
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGGCCAAAGTCTTCTCACCTGGGTCCTAGCCGCGGCAGTCACCTGCGCCCAGGCACAGGATGTGC CGCCGTGGACAGAAGATTGCAGAAAATCAACGTATCCTCCTTCTGGACCAACCTATAGAGGACCAGTTCC GTGGCACACCATAAATCTTGATTTACCACCCTACAAAAGATGGCATGAATTATTGGCTCAAAAGGCACCA GCGTTGAGGATTTTAGTGAATTCCATAACGAGTTTAGTGAATACATTTGTGCCAAGTGGAAAACTAATGA AGATGGTGGATCAAAAGCTGCCTGGTATGATTGGCAGCCTTCCTGACCCTTTTGGAGAGGAAATGAGGGG AATTGCAGATGTTACTGGGATTCCTCTAGGAGAGATTATTTCATTCAACATTTTCTATGAATTGTTTACC ATGTGTACATCAATCATAACTGAAGATGAGAAAGGTCATTTACTACATGGGAGAAACATGGATTTTGGAA TATTTCTTGGGTGGAATATAAATAATAACACTTGGGTTGTCACAGAAGAATTAAAGCCCTTAACAGTGAA TTTGGACTTCCAAAGAAACAATAAGACTGTTTTCAAGGCTACAAGTTTTGTTGGATATGTGGGCATGTTG ACAGGATTCAAACCAGGACTGTTCAGTCTTTCACTAAATGAACGTTTCAGTATAAATGGTGGTTATCTGG GTATCCTAGAATGGATGTTCGGAAGGAAAGATGCCCAGTGGGTAGGGTTTATCACTCGATCAGTTCTGGA AAACACCACAAGTTATGAAGAAGCCAAGAACACACTGACCAAGACCAAGATAATGGCGCCAGTATATTTT ATCCTGGGAGGCAAGAAGTCTGGAGAGGGTTGTGTGATCACACGGGAAAGAAAAGAGTCTTTGGATGTCT ATGAACTTGATCCTAAGCATGGCAGATGGTATGTGGTACAAACCAATTATGACAGGTGGAAAAACACCTT GTTTATTGATGACCGCAGAACACCGGCCAAGAAGTGTCTAAATCACACCACACAGAAGAATCTCTCCTTT GCTACCATCTATGATGTCCTATCAACAAAACCTGTCCTCAACAAGCTGACTGTATTCACAACCTTGATGG ATGTTACCAAAGGTCAATTTGAAAGTCACCTTCGAGATTGCCCAGACCCTTGTATAGGCTGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_019734 |
| Insert Size | 1185 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019734.3, NP_062708.1 |
| RefSeq Size | 2264 bp |
| RefSeq ORF | 1185 bp |
| Locus ID | 11886 |
| UniProt ID | Q9WV54 |
| Gene Summary | This gene encodes acid ceramidase, an enzyme that plays a central role in ceramide metabolism. The encoded protein undergoes proteolytic processing to generate a heterodimeric enzyme comprised of alpha and beta subunits that catalyzes the hydrolysis of sphingolipid ceramide into sphingosine and free fatty acid. The homozygous disruption of this gene leads to embryonic lethality in mice whereas the heterozygous animals exhibit a progressive lipid storage disease phenotype. [provided by RefSeq, Oct 2015] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG206162 | Asah1 (tGFP-tagged) - Mouse N-acylsphingosine amidohydrolase 1 (Asah1) |
CNY 5,256.00 |
|
| MR206162 | Asah1 (Myc-DDK-tagged) - Mouse N-acylsphingosine amidohydrolase 1 (Asah1) |
CNY 2,850.00 |
|
| MR206162L3 | Lenti ORF clone of Asah1 (Myc-DDK-tagged) - Mouse N-acylsphingosine amidohydrolase 1 (Asah1) |
CNY 4,750.00 |
|
| MR206162L4 | Lenti ORF clone of Asah1 (mGFP-tagged) - Mouse N-acylsphingosine amidohydrolase 1 (Asah1) |
CNY 4,750.00 |
