Entpd5 (BC015247) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	mNTPase, NTPDase-5, NTPDase5, ER-UDPase, Pcph
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC015247 GGCAGGCAGGAGCTGCTTTTTAAATTGGCCTGCGTGACCCGCCCACTTGGTGTAAAAGAAGAACCGGCCA AAGGGAGGGCCTGAAGGACCTCCACAGGAGTGTGAGCAGCACTGCTTCAGCAACAAAGCCTCAGGTCCAC ATCTTGGGAAGAATATGGCCACTTCCTGGGGGGCTGTCTTCATGCTGATCATAGCCTGCGTTGGCAGCAC TGTCTTCTACAGAGAACAGCAGACCTGGTTTGAAGGTGTCTTCTTGTCTTCCATGTGCCCCATTAATGTC AGTGCCGGCACCTTTTATGGAATTATGTTTGATGCGGGCAGCACTGGAACTCGGATTCATGTTTACACTT TTGTGCAGAAAACAGCAGGACAGCTCCCCTTTCTGGAAGGTGAAATTTTTGATTCTGTGAAGCCGGGACT TTCTGCTTTTGTGGATCAGCCCAAACAGGGTGCTGAGACTGTCCAGGAGCTCTTGGAGGTGGCCAAAGAC TCGATCCCCAGAAGCCACTGGGAAAGGACCCCGGTGGTTCTGAAAGCAACGGCCGGACTCCGTTTGCTGC CTGAGCAGAAAGCCCAGGCTCTGCTCTTGGAGGTAGAGGAGATCTTCAAGAATTCACCTTTCCTGGTCCC AGATGGCAGCGTTAGCATCATGGATGGGTCCTATGAAGGCATACTAGCCTGGGTTACCGTGAACTTTCTA ACAGGTCAGCTGCATGGTCGTGGCCAGGAGACTGTGGGGACCCTTGACCTGGGGGGTGCCTCCACCCAAA TCACGTTTCTACCCCAGTTTGAGAAAACCCTGGAACAAACACCTAGGGGCTACCTCACTTCCTTTGAGAT GTTTAACAGCACTTTTAAGCTCTATACACATAGTTACTTGGGATTTGGACTGAAAGCTGCAAGACTGGCA ACTCTGGGAGCCCTGGAAGCAAAAGGGACTGATGGACATACGTTTCGAAGTGCCTGTTTACCAAGATGGT TGGAAGCAGAGTGGATCTTTGGGGGTGTGAAATACCAGTATGGTGGTAACCAAGAAGGGGAGATGGGCTT TGAACCCTGCTATGCGGAAGTGCTGAGGGTAGTACAGGGGAAACTTCACCAGCCAGAAGAAGTCCGAGGA AGCGCCTTCTACGCTTTCTCTTACTACTACGATCGAGCCGCTGACACACACTTGATCGATTATGAAAAGG GCGGGGTTTTAAAAGTTGAAGATTTTGAAAGAAAAGCCAGAGAAGTGTGTGACAACTTGGGGAGCTTCTC CTCGGGCAGTCCTTTCCTCTGCATGGACCTCACTTACATCACAGCCCTGTTGAAAGATGGTTTTGGCTTT GCCGACGGCACCCTCTTACAGCTCACAAAGAAAGTGAACAACATAGAGACTGGTTGGGCCTTGGGGGCCA CCTTTCACCTGCTCCAGTCTCTGGGCATCACCAGCTGAGGCCAAGCTCCACCTCTGAAGCCTGCATTTCT GAACAGTTTTCTCACAGGAAGGCGTGGACTCAGAGACATTTTCTGACCTCTCTGGAAAAGCCTGGCCCGA AACCCGTTAACTGGTTTTATAAGGAGGGAGGGGTTTTTAGATGAGTCTTGCTCTTGAGCCTAGTGATTTG GGCTTCAATGATTTGCACATCTAATGTGAATAGCTCCTAACCACTTGGTGGGTGCATGGCTGGCACCAGA CTGTAAATCTTTTGGGATTCTTTGTTACAGAGTCCTGCAAAGGAAAAAAGAGAAAAGGTTTGGAACTCCA TGCTAGATTGCGAGTTCAGAGACAGGTCCCTGGGGACCAAAGAACAATCTCGTTTCAACCCTTGGATGCC TCATTGCTTTGAATGGATTCATTTTTGCTTATAAGCTGATTTACTGAAATCCCATAACCCATCAATGCTG TTAATTTTTTTCTTCCTACCCTTATTACATTCCCTACCCTAAAAGCCTGGGGGAAATACCTGGTTTTGCT TCCCATCTATAATTGAGAAAGAGGGGGGAAAAGATACTGTATTAGAATTTGTGTGATCCTGTGGCACAAT AGATCAACCAACCCATTTAAAGCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC015247
Insert Size	1284 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC015247, AAH15247
RefSeq Size	2099 bp
RefSeq ORF	1284 bp
Locus ID	12499
Gene Summary	Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. The nucleotide hydrolyzing preference is GDP > IDP > UDP, but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.[UniProtKB/Swiss-Prot Function]

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