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Prkag2 (BC015283) Mouse Untagged Clone
CAT＃: MC206815
Prkag2 (untagged) - Mouse protein kinase, AMP-activated, gamma 2 non-catalytic subunit (cDNA clone MGC:18882 IMAGE:4238045), (10ug)
CNY 4,224.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AAKG, WPWS, AAKG2, H91620p |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC015283
CCCAGCACAAGACTTCTGCCACGCGTAACCAAAAGTAGCACGGGCCGAGACTCTGCACCCAATGATTCCG TAGGAGAAAGAGGACGCTCCAACCCGGCATTCCGCGGACTGCCAGCCCCTCGGGTGGAGCCCCGCGTGTT ACCTGCGCCACCCGCCCTCCCCGCGGAAGTCCCCACGAGGTGTTTTCAGGGTGGGAGGACAGTCATAAAA AGCGCGTGGCTCCAGTGTAACGGCAGCAGTCCTGGGAGGCAGCGAGGGGACCGAACCCCGGGGACGAGGC ACCGGAATTAACTTCTAGCTAAAGTTATGGGAAGCGCAGCCATGGACACCAAGAAGAAGAAAGAGGTTTC CAGCCCGGGCGGGAGCAGTGGCAAAAAGAACCCTAGCCTGAAGAGGCGGTCACTGCGAGTGCACATTCCG GACCTGAGTTCCTTTGCCATGCCGCTCCTGGATGGAGATGTGGAGAATTCAGAAAAGCATTCATCTCGGA AGGTGGACAGTCCCTTCAGCTCAGGCAGCCCCTCCAGAGGACTCTTCTCCAGAGGCCCCCAGCCCCGGCC CTCCAGCCCTGTGTCTGCTCCCGTGAGGCCCAAGACGAGCCCTGGCTCTCCCAAAACTGTGTTCCCGTTC TCCTACCAGGAGTCCCCTCCACGCTCTCCACGCCGGATGAGCTTCAGTGGGATCTTCCGCTCCTCATCCA AAGAGTCTTCGCCCAACTCCAACCCGTCTACCTCTCCTGGGGGCATCCGGTTCTTCTCACGGTCCAGAAA AACCTCCAGCGTCTCCTCTTCTCCGTCAACACCCACCCAAGTGACCAAGCAGCACCCATTTCCCCTGGAG TCCTACAAGCAGGAGCCTGAACGCCCAGAGAGCCGCATCTATGCTTCCTCGTCCCCTCCAGACACTGGGC AGCGCTTCTGCCTGGCCTTTCAGAGCCCAGCGCGACCTCCGCTGGCATCCCCGACATACCATGCTCCTTT GAGAACTGCGGTGCTGGCGGCGGCCCCGGGACCCGCGGAAGCCGGCATGCTGGAGAAGCTGGAGTTCCAG GAGGAAGAAGACTCAGAAAGTGGCTTTTATATGCGATTCATGAGGTCACACAAGTGTTATGACATCGTTC CAACCAGTTCAAAGCTTGTTGTCTTCGACACTACGTTGCAAGTCAAAAAGGCCTTCTTTGCCTTGGTAGC CAACGGAGTCCGTGCAGCGCCGCTGTGGGAAAGTAAAAAGCAGAGCTTTGTAGGAATGCTCACGATTACA GATTTCATAAATATCCTACACAGATACTATAAATCACCCATGGTACAGATTTATGAATTGGAAGAACATA AGATTGAAACGTGGAGGGAGTTGTACTTACAAGAAACCTTCAAGCCTTTGGTGAACATCTCTCCAGATGC GAGCCTCTTCGATGCTGTATACTCGTTGATCAAAAATAAAATCCACAGATTGCCAGTTATTGACCCTATC AGTGGGAACGCACTTTATATACTTACCCACAAAAGAATCCTCAAGTTCCTCCAGCTTTTTATGTCTGACA TGCCAAAGCCTGCCTTCATGAAGCAGAACCTGGATGAGCTTGGAATCGGAACGTACCACAATATTGCCTT CATTCACCCAGACACTCCCATCATCAAAGCCTTGAACATCTTCGTGGAGAGACGGATATCCGCATTGCCT GTGGTGGATGAGTCAGGAAAAGTTGTAGATATTTATTCCAAGTTTGATGTAATTAATCTTGCTGCTGAGA AAACGTACAATAACTTGGACATCACAGTGACCCAGGCCCTGCAGCACCGCTCGCAGTATTTTGAGGGTGT GGTGAAGTGCAGTAAGCTGGAAACACTGGAGACCATCGTGGACAGGATAGTGAGAGCCGAGGTCCATCGG CTGGTTGTAGTGAATGAAGCAGATAGCATTGTGGGTATTATCTCCCTGTCAGACATCCTGCAAGCCCTGA TCCTCACACCAGCAGGTGCCAAACAGAAGGAGACAGAGACTGAATGACCGCCGTGAATGTAGACGCCCGC GCAGGAGAACTTGAACAAAGTCTCGGGGTCACGGTTTGCCTCATGAACACTGGCTGCAATAGAAGAGAGT AAAATGGCTAAGAATGTATACCAGGGTTTAGCGATGGGTATTTTTCCAGTGATTTGTAAGTATAGGAAAA GATTTTATGTGCTTGAAGATTCAGGCTTGCGTTCAAAGACTGCCCTCAGACCTGTCTGAAGGATTTTCAA TGCTGTATGTCATTAAAGTGCACTGTGTCCTGAAATAGTACTACTTTTCATTTCAAAGAAGTCACTGGGA TGGAACAGGTGACAGAAAGTGAGTGAACGGCACGTCCAGATCACGGTGCCTTATGTCCAAACGCAATATG TCACCGCCGCTGCCGCACGCCCCAAAGTGCTGCTGAAGAGACACCCAGCCCAAGTGTGAGCATCTTCAGA CCTTTTACCCAGTCTCTTCCTTTTCACTTAATTGGTTGAAAAGTTTTAATTTGAGTACAGGCAACTGAAA ATGTGATGTGATGCCTCTAAGCGCTCTGCATCCGTCCTGTTCTGGCTGCCGTGTTAGCGAGGCCGGCTTG GCAACAGCGCTTTATTTATTTTTACTCTCATGCAGGTTTCAACCTACATCCTTTGGTGAGTAGACGTCAC CATACCTACGAATAAAACCTTGATTGTTCTCTCAAGTGGAAAATCTCTGGAGATTTTAGTTTGGATCTGG AAAGGCTATGCCTTCCAAACAGCCACTGTAGGGTCTTCTAAACGTTTATGAAAACAGGCTGTGGTCGCAG GGAAGAAGTAACGGAAGACGTCTTTTTTTTTATTGTGTGTTGAAATGTAAACTCATGATGTTTGTGTGAC CGCTGACAAGACGCTTGTAACTTTTATTGTGACACATAAAACAGTATTGTCATGCAGTTGAAGGGAAACT CTTTCCCCTCCCACACCCCACAACCCCCACAGCCCCCAGTGTCAGTGCTCTGAAAATGTTGACACTATAT ATCTGTGTGTGTATATATGAGCAGAGTTTGGGGGTTTGTTTTGGGGTTTTGTTTCGTTTTGTTTTTCAGA TTGAAAATTAAAATAAATCCTGTCTAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | BC015283 |
| Insert Size | 1701 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC015283, AAH15283 |
| RefSeq Size | 3132 bp |
| RefSeq ORF | 1701 bp |
| Locus ID | 108099 |
| Gene Summary | AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive (By similarity).[UniProtKB/Swiss-Prot Function] |
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