Wasf1 (BC016896) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Scar, WAVE-1, WAVE
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC016896 CAGGGGGAAGCGCCGTCCTCTTGCCCACGGACGGGTGAGCTGGTTCAGTGGCTGAGCTCAAGATGCCGTT GGTGAAAAGAAACATCGACCCTCGGCACTTGTGCCACACAGCACTGCCCAGGGGCATTAAGAACGAACTG GAATGTGTAACCAACATTTCCCTGGCAAATATAATTAGACAACTGAGTAGCCTAAGTAAGTATGCTGAAG ACATATTTGGAGAATTATTCAATGAAGCACACAGTTTTTCCTTCAGAGTTAACTCGTTGCAAGAGCGCGT GGACCGATTGTCTGTTAGTGTCACACAGCTTGATCCTAAGGAGGAAGAACTGTCTTTGCAAGATATAACG ATGAGAAAGGCTTTCCGGAGCTCCACAATTCAAGATCAGCAGCTTTTCGACCGCAAGACGTTGCCTATTC CATTGCAAGAAACATACGATGTGTGTGAGCAACCTCCGCCTCTCAATATCCTCACCCCTTACAGGGATGA TGGTAAGGAAGGGCTGAAGTTTTATACCAATCCTTCATATTTCTTTGATCTGTGGAAAGAAAAGATGTTG CAAGATACAGAGGACAAGAGGAAGGAAAAGAGAAAGCAGAAGCAGAAAAATCTAGACCGTCCTCATGAAC CAGAGAAAGTGCCAAGAGCACCTCATGACAGGCGGCGGGAATGGCAGAAGCTGGCCCAAGGTCCAGAGCT GGCTGAGGATGACGCTGACCTCCTACACAAGCATATTGAAGTTGCCAATGGCCCAGCCTCTCATTATGAG ACAAGGCCACAGACATACGTGGATCATATGGACGGATCGTACTCACTCTCTGCCTTGCCATTCAGTCAGA TGAGTGAACTTCTGACCAGAGCTGAGGAGAGGGTCTTAGTCAGACCCCATGAACCTCCTCCGCCTCCACC AATGCATGGAGCAGGAGATGCAAAGCCCACACCCACCTGTATCAGTTCTGCTACAGGTTTGATAGAGAAT CGCCCCCAGTCCCCAGCTGCAGGCAGAACACCTGTGTTTGTGAGCCCCACTTCCCCACCTCCTCCCCCAC CTCTTCCGTCTGCCTTGTCCACTTCTTCACTAAGAGCTTCAATGACCTCAACTCCTCCCCCACCAGTCCC TCCTCCGCCTCCTCCCCCAGCCACTGCTTTGCAGGCCCCAGCAGTGCCCCCACCTCCGGCTCCTCTTCAG ATTGCCCCTGGTGTTCTTCACCCAGCTCCTCCTCCAATTGCTCCTCCTCTAGTGCAGCCCTCTCCCCCAG TAGCTCGAGCTGCCCCAGTATGCGAGACTGTGCCAGTTCACCCACTCCCGCAAGGTGAAGTGCAGGGGCT GCCTCCACCCCCACCACCGCCTCCTCTACCTCCGCCTGGCATCCGGCCATCATCTCCTGTCGCAGTTGCA GCTCTTGCTCATCCTCCCTCTGGGCTCCATCCAGCACCATCTACTGCCCCGGGTCCCCATGCTCCATTAA TGCCTCCATCTCCTCCATCACAAGTTCTACCTGCCTCTGAGCCAAAGCGCCATCCATCCACCCTGCCTGT AATCAGTGACGCAAGGAGTGTGCTGCTGGAAGCCATCCGAAAAGGTATTCAGCTTCGCAAAGTGGAAGAG CAGCGTGAACAGGAAGCAAAACACGAGCGGATTGAAAACGACGTGGCCACCATCCTGTCTCGCCGCATTG CTGTGGAGTATAGCGACTCGGAAGATGACTCGGAGTTCGATGAAGTAGACTGGCTGGAGTAAGAAAGCCA CAGTGACTTGGAAGATGACTCGGAGTTCGATGAAGTAGACTGGATGGAGTAAGACAGCCGCCTTGCTCTG TATTGCAAGGCTGAATGCACATGTCCCTCGTGGCGCTTGTTCTTTGAAAATGTTCGGTCATTCTAGTGTT TTGCTTCCTATTCCTCATAATGAATAACCCTTTTCCTCCATTATTTTTGATTTCTATGAAAAGTATTAGC ATATATTTCAAACTAAATATTTTGTGGCTTACCTTAATTTTTCCCCCTGGAAAAATATAATTTGGTCCAA TAAACTACTAAGTATTAAGCATGGAAAGCCGTTATTAGAGTAGCAGATTCAGTTTTTGATATTCCTTAAT TGTGCACTTTGTGATTTTTTTTTTTTTTTTTAAATTTGAAGAACGCAACTGAGACTGGAATCTTGAAGGC AGCTGTGTCTGGGAACGAGCCTTATTCCGTTTCTTGATGTTTTAAATGAAGAAACACTGCCTCGATTATA TGAATACACTCAGAAGGAACATGTAGCTTGTAGTGTTGAATTCTCTTAAAGGAATGGTTAGCTTTGGTTA TCATTTTGTTGTTTTTGAATACTTGCCTTATTTGAACGTTTAGCAGAACCCCTTCCCACTTATATATTGT GTGGTACAGTTTTGCTTGCCTATAGAAGTTTAAATTTTTCCATGTGAAATACTCAGAATAAGCATATGTG TGACTTACATAATGTGAGAATTACAATCTGATGTAATAAACGGTTCATTTTAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC016896
Insert Size	1680 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC016896, AAH16896
RefSeq Size	2520 bp
RefSeq ORF	1680 bp
Locus ID	83767
Gene Summary	Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex (By similarity). As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (PubMed:27605705).[UniProtKB/Swiss-Prot Function]

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