Ube2e3 (BC011477) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Ubce4; ubcM2
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC011477 CTTGCTGACTGCGCGACCGGGAGGAGCCGAGCCGAGCCGAGCCGAGCGCAGCGGCGGGAGGCTGAGCGCG CGGGGGGCTCCCGCGTCCCTGCGCCTTCGCCTTCGGCCGGCAGAGCGCCCACCCCACCGTGGCTCCCGGA GCGCGGGGCGGCGCCACCCTCCGGCCGCAGCGCGGCACTGCACGGCCGCCTGCGACTTTCGATGTTCCAC ACTCCCGGGCCGGAGCCTCGGCGGCGGCTTCTTTTCCCGCCCCCCCGGTGCCCCCCCCAGGCTGCCTCCA TTTCCTCAAGGAAGGTTCCTCGGCGCTCTCCCTCCCCCACAGCGTAGCGGCGCACGAGCGGGCCGGGCGG GCGGCCGAGAAGTTTTCCCAGAGATAAGTTCACCAAGATGTCCAGTGACAGGCAAAGGTCGGATGATGAG AGCCCTAGCACCAGCAGTGGCAGTTCAGATGCAGATCAGCGAGACCCTGCCGCTCCAGAGCCCGAGGAAC AGGAAGAAAGAAAACCTTCTGCCACCCAGCAGAAGAAAAACACCAAACTCTCTAGCAAAACGACTGCTAA GTTATCCACTAGTGCTAAAAGAATTCAGAAGGAGCTAGCAGAGATAACCCTTGATCCTCCTCCTAACTGC AGTGCTGGGCCTAAAGGAGATAACATTTATGAATGGAGGTCAACTATACTTGGTCCACCAGGTTCTGTAT ATGAAGGTGGTGTTTTTTTTCTGGATATCACATTTTCTTCAGATTATCCATTTAAGCCACCAAAGGTTAC TTTCCGTACCAGAATCTATCACTGCAACATCAACAGTCAGGGAGTCATCTGCCTGGATATTCTGAAAGAC AACTGGAGTCCTGCTTTGACTATTTCAAAGGTTTTGCTCTCTATTTGTTCCCTTTTGACAGATTGCAACC CTGCGGATCCTCTGGTCGGAAGCATAGCCACTCAGTATTTGACCAACAGAGCAGAACATGACAGGATAGC CAGACAGTGGACCAAGAGATACGCAACATAATTCACATCATTTGTATGCAGTGTGAAGGAGCAGAAGGCC TCTTCCCACTGTGCTGCGGATCTTTATAGCCTTTACAATACGGACTTCTGTGTATATGTTATACTGATTC TACTCTTGTCTTTATCCTTTGGAGACTGGGAGACTCCCCCAAAAGGTAAAAGCTACCAAGAGTAGAACTT TGTAGCTGTAGATTAGTTATGTTTAAAATGCCTACTTGCAAGTCTTGCTTCTTTGGGATATCAAAATGTA TTTTGTGATGTACTAAGGATACTGGTCCTGAAGTCTACCAAATATTATAGTGCATTTTAGCCTAATTCAT TATCTGTATGAAGTTATAAAAGTAGCTGTAGATGACTAGGAATTATGTCATTTGTATTAAGCCCAGATCT ATTTCTGAGTATGTGGTTCATGCTGTTGTGAAATGTTTTACCTTTGTCAGTTTGTAATGAGAGGATTTCC TTCTACCCTTTGTAGCTCAGAGAGCACTGATGTATCATCTCAAACACAATAAACATGCTCCTGAAGGCAA AAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC011477
Insert Size	624 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC011477, AAH11477
RefSeq Size	1562 bp
RefSeq ORF	624 bp
Locus ID	22193
Gene Summary	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination (By similarity). Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest.[UniProtKB/Swiss-Prot Function]

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