Rbbp7 (BC003785) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA409861; AI173248; AU019541; BB114024; mRbAp46
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC003785 GTTCCAAGTTTGGCGAGAGGGAGAGAGAGGAGAGCGGCTCAGACCTCGCTACCCGCCAGCGGGGAGGAGG CAGAAGAGGAGATCGCGGCGTCTGGGGGGAGAACCCAGACGGCCAGACCGAACTCAGGCTTTTCCGAGCG AGGACTGCGTGACGTGCCTGGGAGAGGCAAGGAGCGCCTGCCGGGCTGCTCTTGACTAGCGAGAGAGAAG TCCGAGGCGGCCAAGGGGGGCGAAACGACCCGACGCAAGATGGCGAGTAAAGAGATGTTTGAAGATACTG TGGAGGAGCGTGTCATCAACGAAGAGTATAAAATCTGGAAGAAGAATACACCGTTTCTGTATGACCTGGT TATGACCCATGCTCTTCAGTGGCCCAGTCTTACCGTTCAGTGGCTTCCTGAAGTGACTAAACCAGAAGGA AAGGATTATGCCCTTCATTGGCTAGTGCTGGGCACTCATACATCTGATGAGCAGAACCATCTGGTGGTTG CTCGAGTTCATATTCCCAATGATGATGCACAGTTTGATGCTTCCCACTGTGACAGTGACAAGGGAGAATT CGGTGGCTTTGGTTCTGTAACAGGGAAAATTGAATGTGAAATTAAAATTAACCATGAAGGAGAAGTGAAT CGTGCTCGTTATATGCCACAGAATCCTCACATCATTGCCACAAAAACACCATCTTCTGATGTTTTGGTTT TTGACTATACAAAACACCCTGCAAAACCAGATCCAAGTGGAGAATGTAATCCTGATCTTAGATTAAGAGG TCACCAAAAGGAAGGCTATGGTCTTTCCTGGAATTCTAATCTGAGTGGGCATCTCCTGAGTGCATCTGAT GACCATACTGTCTGCCTGTGGGATATAAATGCAGGACCAAAGGAAGGCAAAATTGTGGATGCTAAAGCAA TCTTTACTGGCCACTCAGCTGTTGTAGAGGATGTGGCCTGGCATCTGCTGCATGAGTCCTTGTTTGGATC TGTTGCTGATGATCAGAAACTTATGATATGGGACACCAGATCCAATACCACTTCTAAGCCGAGCCATTTG GTGGATGCACACACCGCTGAGGTCAACTGCCTCTCATTCAATCCCTACAGCGAGTTCATTCTGGCAACTG GCTCTGCAGATAAGACTGTAGCTTTATGGGACCTGCGTAATCTGAAACTAAAACTCCACACCTTTGAATC GCATAAGGATGAAATTTTCCAGGTCCACTGGTCTCCACATAATGAAACTATTCTGGCCTCAAGTGGTACT GATCGCCGCCTGAATGTGTGGGATTTAAGTAAAATTGGAGAAGAACAATCAGCAGAAGATGCAGAAGATG GGCCTCCAGAGCTCCTGTTTATTCATGGAGGGCACACTGCCAAGATTTCTGACTTCAGCTGGAATCCCAA TGAACCTTGGGTCATTTGCTCTGTGTCTGAAGATAACATCATGCAGATATGGCAGATGGCTGAAAATATT TACAATGATGAAGAGTCAGATGTCACGGCATCGGAACTGGAGGGGCAAGGATCTTAAACCCAAAGTATGA GTTGTTTTTATTGAATGTATTGCTACATGAATGCTTGATTTGTCAAGCGCCAAAAAGGCATTGTATAGTA GGAAATGTAAGTGGGACGGCTTCTGGCATTCTTTACCCTCTGATTCTAGCACTTTCAAGTGAGCTGTTGC GTACTGTATCATATTGTAGCTATTAGGGGATAAAAAGGAATGTTGCTTAAGAACGGACATCATTGTTTTA AGACACACATTGCTGGGTATTGCCTTTGATTCAACTGTCATTTTCTCAAACTTTTAAAGTGCTTGCTCAA GTTCCCAAATATGCGAGGATAACTTTTACACTTTTTCCTTCCAACACTTCTTGATTGGCTTTGCAGAAAT AAAGTTTTAAAATAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC003785
Insert Size	1278 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC003785, AAH03785
RefSeq Size	1918 bp
RefSeq ORF	1278 bp
Locus ID	245688
Gene Summary	Core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the type B histone acetyltransferase (HAT) complex, which is required for chromatin assembly following DNA replication; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; and the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex (By similarity).[UniProtKB/Swiss-Prot Function]

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