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Aco1 (BC005454) Mouse Untagged Clone
CAT＃: MC206784
Aco1 (untagged) - Mouse aconitase 1 (cDNA clone MGC:6247 IMAGE:3494686), (10ug)
CNY 6,520.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Aco-1; Irebp; Irp1 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC005454
CACCCGCCTGTGTGCTCCGGGAACTGCACTGTGAGCGTTTTTCAGAGACACGTGACCATCAATAAGCATG AAGAACCCATTTGCACACCTTGCTGAGCCCTTGGACGCTGCCCAACCAGGAAAGAGGTTCTTCAACTTGA ATAAATTGGAGGACTCAAGATACGGACGCTTACCATTTTCTATCAGAGTTCTCCTGGAGGCAGCCGTTCG GAACTGTGATGAGTTTCTGGTGAAGAAAAATGACATCGAGAATATCCTGAATTGGAATGTCATGCAACAT AAGAACATAGAAGTGCCGTTTAAGCCAGCCCGAGTCATACTGCAAGACTTTACGGGTGTGCCTGCTGTGG TGGATTTTGCAGCAATGCGCGATGCTGTGAAGAAGTTGGGAGGGAATCCGGAGAAAATTAACCCTGTCTG CCCCGCTGACCTTGTAATTGATCATTCCATCCAGGTTGATTTCAACAGAAGGGCAGACAGTTTACAGAAG AATCAAGACCTGGAGTTTGAAAGAAATAAAGAACGATTTGAATTTCTAAAGTGGGGTTCCCAGGCCTTTT GCAACATGCGGATAATCCCTCCTGGCTCGGGAATCATTCACCAAGTGAACCTGGAGTATTTGGCAAGAGT AGTGTTTGATCAGGATGGATGCTACTACCCAGACAGCCTCGTGGGCACAGATTCACACACGACCATGATT GACGGCCTGGGAGTTCTTGGTTGGGGTGTGGGTGGTATTGAAGCAGAAGCTGTCATGCTGGGTCAGCCCA TCAGCATGGTGCTTCCCCAGGTGATTGGCTACAAGCTGATGGGGAAGCCTCACCCTCTGGTAACATCCAC GGACATCGTGCTCACCATTACCAAGCACCTCCGACAAGTGGGAGTTGTGGGCAAATTTGTGGAGTTTTTC GGGCCAGGAGTGGCTCAGCTGTCCATTGCTGACCGAGCTACGATTGCCAATATGTGCCCAGAGTATGGTG CGACAGCAGCCTTCTTCCCGGTTGATGAAGTTAGCATCGCGTACCTGCTGCAGACAGGCCGCGAGGAAGA CAAAGTCAAGCACATTCAGAAGTATCTTCAGGCTGTAGGCATGTTTCGAGATTTCAACGACACCTCTCAA GACCCAGACTTCACTCAGGTTGTGGAGTTAGATCTGAAAACAGTTGTGCCTTGCTGCAGTGGACCCAAAA GACCTCAGGACAAAGTCGCAGTGTCTGAGATGAAAAAGGACTTTGAAAGCTGCCTTGGAGCCAAGCAAGG ATTTAAAGGTTTCCAAGTTGCTTCAGACCGTCACAATGACCGCAAGACGTTCCTCTATAATAACAGTGAA TTCACTCTCGCTCACGGCTCTGTGGTAATCGCTGCCATCACTAGCTGCACAAACACCAGCAATCCATCCG TGATGTTAGGAGCAGGATTGTTAGCAAAGAAAGCTGTGGAGGCGGGGCTGAGTGTGAAGCCTTACATCAA AACCAGCCTGTCTCCTGGAAGTGGAGTGGTCACCTACTACCTTCGAGAGAGTGGAGTCATGCCTTACCTG TCCCAGTTAGGGTTTGATGTGGTGGGCTACGGCTGCATGACCTGCATCGGCAACAGTGGACCCCTTCCTG AACCTGTGGTCGAGGCGATCACCCAGGGAGACCTCGTAGCTGTTGGCGTTCTGTCTGGGAATAGGAATTT TGAAGGCCGAGTCCATCCTAACACACGAGCCAACTACTTAGCATCCCCCCCTCTGGTAATAGCATATGCG ATTGCAGGCACCGTCAGGATTGACTTCGAGAAAGAGCCTTTGGGAGTGAACGCACAGGGCCGGCAAGTGT TTCTGAAGGATATCTGGCCCACGCGAGATGAGATCCAGGCGGTGGAAAGGCAGTATGTCATCCCCGGGAT GTTTAAGGAGGTCTATCAGAAAATAGAGACTGTAAATAAAAGCTGGAATGCCTTAGCAGCCCCGTCAGAG AAGCTGTACGCGTGGAATCCCAAATCTACTTACATTAAGTCGCCGCCATTCTTTGAAAGCTTGACTTTAG ATCTCCAGCCACCCAAGTCCATAGTGGATGCCTATGTGCTACTGAATTTAGGAGATTCAGTAACAACGGA CCATATCTCTCCCGCCGGCAATATTGCAAGAAACAGCCCTGCGGCTCGCTACTTGACGAACAGAGGCCTA ACACCACGAGAGTTCAACTCCTATGGCTCCCGCCGGGGTAACGACGCCATCATGGCCCGGGGGACATTTG CCAACATTCGCTTGCTGAACAAGTTTCTGAACAAGCAGGCACCTCAGACTGTCCATCTCCCTTCAGGAGA AACCCTTGATGTATTCGACGCTGCTGAGCGGTACCAGCAGGCTGGACTTCCCCTGATTGTTCTGGCTGGG AAAGAATACGGTTCAGGCAGCTCCCGAGACTGGGCAGCCAAAGGCCCTTTCCTGCTGGGAATCAAAGCTG TCCTGGCAGAGAGCTATGAGCGCATTCACCGCAGCAACTTGGTTGGCATGGGGGTGATCCCCCTTGAGTA TCTCCCTGGTGAAACTGCAGACTCTCTGGGACTCACAGGTCGGGAAAGATACACTATCAACATCCCTGAA GACCTCAAGCCTCGCATGACGGTGCAGATCAAGCTGGACACTGGGAAGACCTTCCAGGCCGTGATGAGGT TCGACACTGATGTGGAGCTCACATACTTCCACAATGGAGGCATCCTGAACTACATGATCCGAAAGATGGC CCAGTAGGTGCTGGCCTCTCAGGAGACCCGCGCTTGGTGCTAGACCCAATGAGGAACCAGGCCCCCACTG GTGGAGGCCTGGCAGAGCAGCCACATCTACTTCTGATGAGGGTGCTGGCAGGATGAGCAAGTGGGCACTG CCATTCCTGGAGGCACAGAGCCAGGAGTCTCTAGTTTCGTGATTTGTTCATCTTTTTATCCCTTTCTGTA ATCTGGATTCTAGAATCACGGGAAGGTCCGTAGTACCAAAGAGAACTCGCTTCTCTTTAAAGTCACGGGC ATTGATTTTTTTCCCTCTGACTAATCTTGAGCTCAGCACTAGCCAGTATTCTCAGAAGTGGCTCCTACCC TTTCTGTTGTTCTGTGTTTGTCTCTGCTCGGTGACACCCTTCCCTGGAGAGCCCAGTCCTCCGTGTATTA CACCAGTGTTAACTGACATAGCATAAGACCTTCACACTTCAGATTCATAGCAGTGATGGGATCCCTTCCT TTGAAGTGAACAAGTCCTTGTAGATAGGTCACCCGTCAAAAGTGTTTGATTATCTACCTTTCAATCAACA TGAGATTGCCTCTTACCATTTTCAACGATTGTTGACAGGGGTCCTTTGTTTGAAAATAACTGGGGAGAGA TACGGGATTTCTAATTTGAATAAGATTAAATATATTTTCATGAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | BC005454 |
| Insert Size | 2670 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC005454, AAH05454 |
| RefSeq Size | 3420 bp |
| RefSeq ORF | 2670 bp |
| Locus ID | 11428 |
| Gene Summary | This gene encodes a member of the aconitase/IPM isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Depending on iron levels in the cytosol, the encoded protein can function as either an aconitase enzyme or as an mRNA binding protein. When cellular iron levels are high, the encoded protein functions as an aconitase, an essential enzyme in the TCA cycle that catalyzes the conversion of citrate to isocitrate. When cellular iron levels are low, the encoded protein regulates iron uptake and utilization by binding to iron-responsive elements in the untranslated regions of mRNAs for genes involved in iron metabolism. Disruption of this gene is associated with pulmonary hypertension and polycythemia. [provided by RefSeq, Jan 2014] |
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