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Polr2b (BC038472) Mouse Untagged Clone
CAT＃: MC206769
Polr2b (untagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide B (cDNA clone MGC:47046 IMAGE:5026936), (10ug)
CNY 8,136.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | RPB2, POL2RB, RPB140, MGC47046 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC038472
CCACGCGTCCGGGAACGTCGGAGACGGACATTGCTTGGTATCGCACTCTTGAGTTTGCCGCCGCGCTTCC CGTCTCCAGCTTTGCGATTCCGAGTAAGAGCTCCAGAAACCTTTGGCCAAGATGTATGACGCCGACGAGG ACATGCAATATGATGAAGACGACGATGAGATCACTCCGGATCTGTGGCAGGAAGCATGCTGGATCGTGAT CAGTTCTTACTTTGATGAGAAAGGCTTGGTCAGACAACAGCTGGATTCCTTTGATGAGTTTATTCAGATG TCTGTTCAAAGAATTGTGGAAGATGCACCACCGATTGACCTACAGGCCGAAGCTCAGCACGCCAGTGGAG AAGTTGAAGAGCCGCCAAGATATTTACTGAAGTTTGAACAAATTTACCTCTCCAAACCTACTCATTGGGA AAGAGATGGTGCCCCTTCGCCAATGATGCCAAATGAAGCCAGGTTAAGAAATCTCACGTATTCTGCTCCG CTTTACGTTGACATAACAAAGACAGTCATTAAAGAAGGTGAAGAACAGCTGCAGACTCAGCACCAGAAAA CGTTCATAGGGAAAATTCCAATTATGCTGCGCTCCACCTACTGCCTGTTAAATGGCTTAACAGATCGTGA CCTGTGTGAGCTGAATGAGTGTCCTTTGGATCCCGGTGGCTATTTTATTATCAATGGATCAGAAAAGGTT CTGATAGCTCAAGAGAAAATGGCAACAAACACAGTCTATGTGTTTGCCAAAAAGGATTCCAAGTATGCCT ATACAGGAGAATGTCGCTCATGCCTGGAGAATTCTTCCCGACCCACCAGTACTATATGGGTTAGCATGCT GGCCAGAGGAGGACAGGGTGCAAAGAAGAGTGCTATTGGCCAGCGCATCGTGGCAACGTTACCATACATC AAGCAAGAAGTTCCTATCATCATTGTGTTCAGAGCACTAGGCTTTGTGTCTGATAGAGATATTTTAGAAC ACATCATTTATGACTTCGAGGATCCAGAGATGATGGAAATGGTTAAACCATCTCTCGATGAAGCGTTTGT CATCCAAGAGCAGAATGTTGCCTTAAACTTCATCGGTTCAAGAGGGGCAAAGCCTGGTGTTACTAAGGAG AAAAGAATTAAATATGCAAAAGAAGTCTTACAGAAAGAAATGCTCCCTCACGTTGGTGTCAGTGATTTCT GTGAGACCAAAAAGGCCTATTTCTTGGGGTATATGGTTCATAGGTTGCTTCTAGCAGCCCTAGGCAGAAG AGAACTAGACGACAGAGATCACTATGGCAACAAGAGATTGGATCTTGCTGGGCCTCTGCTTGCATTCTTA TTCAGAGGCATGTTTAAGAATTTGCTGAAAGAAGTAAGAATCTATGCGCAGAAGTTTATTGATCGCGGGA AGGATTTTAACTTAGAGCTGGCAATCAAGACAAGGATCATATCTGATGGGCTAAAATACTCTTTAGCCAC TGGGAACTGGGGTGACCAGAAGAAAGCTCATCAAGCCAGAGCTGGAGTATCCCAGGTATTGAACCGCCTG ACGTTTGCATCTACTCTTTCTCACCTGCGTCGTCTAAACTCTCCTATTGGCAGAGATGGCAAGCTAGCGA AGCCCAGACAGTTGCATAATACCTTGTGGGGAATGGTGTGTCCTGCTGAAACTCCAGAGGGCCATGCTGT AGGGCTTGTGAAGAATTTAGCCCTGATGGCTTATATTTCAGTTGGATCTCAACCCTCTCCAATTTTGGAA TTTTTAGAAGAATGGAGCATGGAAAATTTAGAAGAAATTTCTCCTGCAGCTATTGCTGATGCAACCAAGA TTTTTGTTAATGGCTGCTGGGTTGGAATCCATAAAGACCCCGAACAACTCATGAACACGCTAAGGAAGTT ACGGCGGCAGATGGACATCATTGTATCCGAAGTTTCTATGATCAGAGATATTCGAGAGCGGGAGATTCGG ATCTATACAGATGCAGGCCGTATTTGTAGACCCCTTCTGATTGTGGAAAAGCAGAAGCTGCTTTTGAAGA AGAGACACATTGACCAGTTGAAAGAGAGAGAATATAACAACTACAGTTGGCAGGATCTGGTAGCCAGTGG GGTAGTAGAGTATATTGATACCCTGGAAGAGGAGACCGTGATGCTTGCAATGACTCCAGATGATTTGCAG GAGAAGGAAGTAGCTTATTGTTCTACCTACACCCACTGTGAGATTCACCCATCAATGATTCTTGGAGTCT GTGCATCTATTATTCCTTTTCCTGATCATAACCAGTCCCCTAGAAACACATACCAGTCTGCTATGGGCAA GCAGGCTATGGGAGTTTATATCACCAACTTCCATGTGCGGATGGACACCTTGGCCCATGTTCTCTACTAT CCTCAGAAACCCCTTGTGACTACACGGTCTATGGAATATCTACGATTCAGAGAGCTGCCAGCAGGAATCA ACTCAATTGTGGCCATTGCATCCTACACGGGCTATAATCAAGAAGACTCTGTTATCATGAATCGTTCAGC TGTGGATCGAGGCTTTTTCAGGTCTGTTTTCTACCGATCATACAAAGAACAAGAGTCTAAAAAGGGATTT GATCAAGAAGAAGTGTTTGAGAAGCCTACACGTGAGACATGCCAGGGAATGAGGCATGCCATCTATGAAA AGTTGGACGATGACGGTTTGATAGCTCCGGGGGTCCGAGTCTCCGGAGATGACGTTATTATCGGCAAGAC AGTCACCTTACCCGAGAACGAGGATGAGCTGGAGAGCACCAACAGGCGCTACACCAAGAGGGACTGCAGC ACTTTCCTCAGAACTAGTGAGACGGGCATCGTGGACCAGGTCATGGTCACTCTCAACCAGGAAGGATATA AATTTTGTAAAATAAGGGTGCGCTCTGTTAGAATTCCGCAGATTGGAGACAAATTTGCTAGCCGACATGG TCAGAAGGGAACTTGTGGTATTCAGTATAGACAAGAGGACATGCCGTTCACTTGCGAAGGCATAACTCCT GACATCATCATAAACCCCCACGCCATCCCCTCCCGGATGACAATTGGTCACTTGATCGAATGTCTCCAAG GGAAGGTCTCTGCCAACAAGGGTGAAATTGGTGACGCTACTCCATTTAATGATGCTGTCAATGTGCAGAA GATTTCTAATCTTTTGTCTGACTATGGCTACCATCTCAGAGGGAACGAGGTGCTCTACAATGGATTCACT GGACGAAAAATCACATCACAGATTTTTATTGGCCCCACTTACTACCAGCGTTTGAAGCATATGGTGGATG ACAAAATTCATTCTCGTGCTAGGGGACCCATTCAGATTCTTAACAGACAGCCCATGGAGGGCAGATCTCG TGATGGTGGCCTGCGCTTCGGAGAAATGGAACGAGACTGTCAAATTGCCCACGGTGCAGCACAGTTTCTA AGAGAAAGACTGTTTGAAGCATCAGATCCGTATCAGGTTCATGTCTGTAATCTTTGTGGAATAATGGCAA TTGCTAACACGAGGACTCATACATATGAGTGCAGGGGATGCCGCAATAAAACCCAGATTTCCTTGGTGCG AATGCCGTACGCGTGCAAGCTGTTGTTCCAGGAGCTCATGTCCATGAGCATCGCACCACGAATGATGAGT GTTTAGCTGCCAGAAGTTCATGGTCAGATACTGTGACTATCACTATCTTGTTTCTGTGATTGTCATTAAA AAAAAATTTACTGTTGTGATAAGAATTTTATTTGTTTTGTTTAAAGCTACATGTGCTTTTTTCTGTAAAT AATAAATTTTTGTAGATAGTCAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | BC038472 |
| Insert Size | 3504 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC038472, AAH38472 |
| RefSeq Size | 3818 bp |
| RefSeq ORF | 3504 bp |
| Locus ID | 231329 |
| Gene Summary | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).[UniProtKB/Swiss-Prot Function] |
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